Amg 176

Manufactured by Chemietek

The AMG-176 is a high-precision laboratory instrument designed for analytical applications. It features advanced temperature control, accurate measurements, and reliable performance. The core function of the AMG-176 is to provide precise and consistent results for a variety of laboratory tasks.

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Lab products found in correlation

Dimethyl sulfoxide (dmso), by Merck Group (3 mentions) Trametinib, by Selleck Chemicals (2 mentions) Venetoclax, by Chemietek (2 mentions) Azd5991, by Chemietek (1 mentions) Degrasyn wp1130, by Selleck Chemicals (1 mentions) Maritoclax, by Selleck Chemicals (1 mentions) Quinoline val asp ome ch2 oph q vd oph, by Apexbio (1 mentions) Mycoalert mycoplasma detection kit, by Lonza (1 mentions) S63845, by Chemietek (1 mentions) Azd2014, by Selleck Chemicals (1 mentions) Mg132, by Merck Group (1 mentions) Mk 2206, by Selleck Chemicals (1 mentions) Cycloheximide (chx), by Merck Group (1 mentions) Trametinib, by LC Laboratories (1 mentions)

4 protocols using amg 176

Evaluation of Novel Anticancer Agents

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AMG-176 and AZD5991 were purchased from Chemietek; MG-132, Degrasyn (WP1130), Maritoclax, and trametinib were purchased from Selleckchem, Houston, TX. Agents were dissolved in DMSO (Sigma-Aldrich, Burlington, MA).

Tantawy S.I., Sarkar A., Hubner S., Tan Z., Wierda W.G., Eldeib A., Zhang S., Kornblau S, & Gandhi V. (2023). Mechanisms of MCL-1 protein stability induced by MCL-1 antagonists in B-cell malignancies. Clinical cancer research : an official journal of the American Association for Cancer Research, 29(2), 446-457.

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Synergistic Evaluation of AMG-176 and Venetoclax

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AMG-176 and venetoclax were purchased from Chemietek (Indianapolis, IN) and Xcessbio Company (San Diego, CA), respectively. For both drugs, stock solutions were made in dimethyl sulfoxide (DMSO; Sigma-Aldrich). The pan caspase inhibitor, quinoline-Val-Asp (Ome)-CH₂-O-Ph (Q-VD-Oph) was obtained from APExBIO (Houston, TX). Typically, incubations were performed for 24 hours and for all treatments, time-matched, DMSO-treated (i.e., vehicle only) cells were used as controls. MEC1 cell line was purchased from Deutsche Sammlung von Mikroorganismen und Zellkulturen (DMSZ, Braunschweig, Germany). It was authenticated using short-tandem repeat profiling and regularly tested for Mycoplasma contamination using Mycoalert Mycoplasma detection kit (Lonza Group Ltd, Basel, Switzerland). We use cells between 5^th and 20^th passage. The cells were used as a reference protein concentration for the Bcl-2 family proteins.

Yi X., Sarkar A., Kismali G., Aslan B., Ayres M., Iles L.R., Keating M.J., Wierda W.G., Long J.P., Sabrina Bertilaccio M.T, & Gandhi V. (2020). AMG-176, an Mcl-1 antagonist, shows preclinical efficacy in chronic lymphocytic leukemia. Clinical cancer research : an official journal of the American Association for Cancer Research, 26(14), 3856-3867.

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Bioactive compound screening protocol

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Trametinib (in vitro), MK2206, AZD2014 and EOAI3402143 were purchased from Selleck Chemicals. Trametinib used for in vivo experiments was purchased from LC Laboratories. S63845 and AMG-176 were purchased from Chemietek. MG132 was used at a final concentration of 10 μM where indicated and was purchased from Millipore (#474790). Cycloheximide was used at a final concentration of 25 μg/ml where indicated and was purchased from Sigma (#C7698). THZ1 was purchased from MedChemExpress. THZ531 was provided by Dr. Nathanael Gray. Drug concentrations are specified in each figure.

Perurena N., Lock R., Davis R.A., Raghavan S., Pilla N.F., Ng R., Loi P., Guild C.J., Miller A.L., Sicinska E., Cleary J.M., Rubinson D.A., Wolpin B.M., Gray N.S., Santagata S., Hahn W.C., Morton J.P., Sansom O.J., Aguirre A.J, & Cichowski K. (2023). USP9X mediates an acute adaptive response to MAPK suppression in pancreatic cancer but creates multiple actionable therapeutic vulnerabilities. Cell Reports Medicine, 4(4), 101007.

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Combination Treatment of AMG-176 and Venetoclax

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AMG-176 and venetoclax were purchased from Chemietek (Indianapolis, IN) and Xcessbio Company (San Diego, CA), respectively. For both drugs, stock solutions were made in dimethyl sulfoxide (DMSO; Sigma-Aldrich). All incubations were for 24 h at the indicated drug concentration alone or in combination. DMSO only was used as a vehicle control.

Yi X., Jain N., Iles L.R., Ayres M.L., Wierda W.G, & Gandhi V. (2022). Targeting Mcl-1 by AMG-176 During Ibrutinib and Venetoclax Therapy in Chronic Lymphocytic Leukemia. Frontiers in Oncology, 12, 833714.

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