Mycological surveillance of ICU patients with severely or critically ill COVID-19 patients who admitted in ICU has been performed only at Seoul St. Mary’s Hospital, since June 2020. To enhance the sensitivity and specificity, serum galactomannan (GM) antigen, (1–3)-β-D-glucan antigen (BDG), and fungal culture from respiratory samples were performed twice a week. Serum GM assay (Platelia Aspergillus EIA, Bio-Rad, Hercules, CA, USA) and BDG test (Gold Mountain River Tech Development, Beijing, China) were performed following the manufacturer’s instructions, using a positivity threshold of ≥ 0.5 optical density index in serum for GM and ≥ 80 pg/mL for BDG. Surveillance for invasive fungal diseases ended after ICU discharge. Diagnosis and treatment of CAPA were determined by an infectious disease physician and an intensive care physician based on consensus criteria.
Platelia aspergillus eia
Manufactured by Bio-Rad
Sourced in France
The Platelia Aspergillus EIA is a diagnostic test used for the detection of Aspergillus antigens in human serum samples. It is an enzyme immunoassay (EIA) that provides a qualitative result to aid in the diagnosis of aspergillosis.
Automatically generated - may contain errors
12 protocols using platelia aspergillus eia
1
Mycological Surveillance of Severe COVID-19 Patients
2
Mycological Diagnosis and Aspergillus Detection
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All clinical samples were analysed after conventional microbiological diagnostic tests had been performed. Routine mycological diagnostic tests consisted of culturing on malt agar (Oxoid, Basingstoke, UK) for seven days at 30 °C. In the case of fungal growth, susceptibility testing was performed by ETEST® for VOR (bioMérieux, Marcy-l′Étoile, France) and itraconazole (Liofilchem, Roseto degli Abruzzi, Italy). Minimal inhibitory concentrations were determined in accordance with EUCAST guidelines version 10.0 [20 ]. Furthermore, GM index was determined via Platelia Aspergillus EIA (BioRad, Marnes-la-Coquette, France) from BAL and serum samples, following the manufacturer’s instructions. Results from BAL samples with a GM index ≥1 and from serum samples with a GM index >0.5 were interpreted as positive.
3
Diagnosis and Management of CAPA
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CAPA was defined according to recently proposed definitions [5 (link)] as well as practice guidelines [20 (link)] using a combination of clinical, radiological, and mycological features of the disease.
Respiratory samples included specimens such us tracheobronchial aspirate (TBA) and/or broncolavage (BAL) (when feasible) and were collected on clinical criteria. Bronchoscopy was not routinely performed and was deemed unfeasible, due to technical difficulties with performing an invasive exam in patients with severe respiratory failure who required CPAP and/or NIV. On respiratory samples, galactomannan (GM) and fungal culture were performed. Fungal cultures were incubated for 7 days at 30 °C on Sabouraud selective media, whereas GM test in serum, BAL and TBA was performed according to manufacturer’s instructions (Platelia Aspergillus EIA, Bio-Rad).
In case of suspected CAPA, the clinical approach was managed together with a dedicated infectious disease specialist (author name, AO). When feasible, chest CT scan was repeated to detect lesions compatible with IPA and was analyzed by dedicated pneumologist and radiologist. In instances of uncertainty, a panel discussion was conducted.
Respiratory samples included specimens such us tracheobronchial aspirate (TBA) and/or broncolavage (BAL) (when feasible) and were collected on clinical criteria. Bronchoscopy was not routinely performed and was deemed unfeasible, due to technical difficulties with performing an invasive exam in patients with severe respiratory failure who required CPAP and/or NIV. On respiratory samples, galactomannan (GM) and fungal culture were performed. Fungal cultures were incubated for 7 days at 30 °C on Sabouraud selective media, whereas GM test in serum, BAL and TBA was performed according to manufacturer’s instructions (Platelia Aspergillus EIA, Bio-Rad).
In case of suspected CAPA, the clinical approach was managed together with a dedicated infectious disease specialist (author name, AO). When feasible, chest CT scan was repeated to detect lesions compatible with IPA and was analyzed by dedicated pneumologist and radiologist. In instances of uncertainty, a panel discussion was conducted.
4
MALDI-TOF MS for Microbial Identification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Isolated colonies from biological samples were identified by the Matrix-Assisted Laser Desorption Ionization–Time Of Flight Mass Spectrometry (MALDI-TOF MS) system (Bruker Daltonik GmbH, Bremen, Germany). Antimicrobial susceptibility tests were performed with Vitek 2 automated system (bioMérieux, Marcy l’Etoile, France) or with the MicroScan system (Beckman Coulter, Brea, CA, USA), according to the manufacturer’s instructions.
Fungal cultures were incubated for 7 days at 30 °C on Sabouraud selective media, whereas GM test in serum and TBA was performed according to manufacturer’s instructions (Platelia Aspergillus EIA, Bio-Rad).
Fungal cultures were incubated for 7 days at 30 °C on Sabouraud selective media, whereas GM test in serum and TBA was performed according to manufacturer’s instructions (Platelia Aspergillus EIA, Bio-Rad).
5
Galactomannan Detection in BAL Specimens
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The Platelia Aspergillus EIA (Bio-Rad, Marnes-la-Coquette, France) was used during routine microbiological workup to detect the presence of galactomannan on uncentrifuged BAL specimens, as described (D'Haese et al., 2012 (link)). The enzyme immunoassay data was expressed as galactomannan index (GMI).
6
Serological Assays for Brucella, Bartonella, and Aspergillus
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Serological assays were applied to serum samples to detect agglutinating anti-Brucella antibodies with BRUCELLACAPT (Vircell, Granada, Spain). A diagnostic Brucella infection titer was 1/160 [13 (link)]. An immunofluorescence assay (Vircell, Granada, Spain) was utilized for testing IgG antibodies against Bartonella quintana, Bartonella henselae, and C. burnetii. Bartonella and Coxiella endocarditis were established when the titer was 1/800 for Bartonella IgG and phase I C. burnetii IgG. Aspergillus galactomannan antigen (GM) was detected using the Platelia Aspergillus EIA (Bio-Rad, Marnes-La-Coquette, France). Patients with an Aspergillus antigen index of greater than 0.5 were classified as positive [14 (link)].
7
Bronchoalveolar Lavage for Aspergillus Detection
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Experienced doctors performed all bronchoscopies with bronchoalveolar lavage (20 ml for three times). The site of bronchoscopy was guided by the lung CT scan at first. Based on the infiltration location on the chest radiograph, the sampling area was selected. If there were diffuse lesions in bilateral lungs, the bronchoscope would be wedged into the middle lobe. If there were multiple lesions in bilateral lungs, the bronchoscope would be wedged into the most serious segment of left and right lung, respectively; then the lavage samples were mixed. If there were multiple lesions in unilateral lung, the bronchoscope would be wedged into the most serious segment or the involved segments and then the lavage samples mixed.
The lavage samples were submitted for direct microscopic examination and microbiological culture. Besides, a vortex was done for the remaining BALF samples and the supernatant was used for GM detection by using the Platelia Aspergillus EIA (Bio-Rad). An OD index ≧ 0.8 for BALF GM was considered positive [2 (link), 11 (link), 12 (link)].
The lavage samples were submitted for direct microscopic examination and microbiological culture. Besides, a vortex was done for the remaining BALF samples and the supernatant was used for GM detection by using the Platelia Aspergillus EIA (Bio-Rad). An OD index ≧ 0.8 for BALF GM was considered positive [2 (link), 11 (link), 12 (link)].
8
Serum Galactomannan Assay for Aspergillosis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Serum GM was measured using the Platelia Aspergillus EIA (Bio-Rad Laboratories, Marnes-la-Coquette, France) according to the manufacturer’s instructions. All serum samples were taken from patients in their neutropenic period, and the first sample was collected at the first occurrence of a fever of >38°C for ≥1 hour. The serum GM level was then measured once weekly until neutrophil recovery. Serum samples were considered positive if the GM index was equivalent to a 0.5 GM antigen level.
9
Aspergillus GM-EIA Testing Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
GM-EIA testing was performed using the Platelia Aspergillus EIA (Bio-Rad), according to the manufacturer's instructions, and all runs were required to meet the designated validity criteria. The GM-EIA was considered positive when the optical density was ≥0.5.
10
Detecting Aspergillus by GM Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To aid clinical diagnosis, GM levels were detected by using the Platelia Aspergillus EIA (Bio-Rad, Marnes la Coquette, France). The BALF-GM and serum-GM assays were performed in accordance with the manufacturer's specifications. The GM index was defined as the ratio of sample OD to control OD. The overall assay results were considered positive if the GM index was ≥0.5 for both serum-GM and BALF-GM results.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!