Nci h460

Manufactured by RIKEN BioResource Center

NCI-H460 is a type of cell line derived from human non-small cell lung cancer. It is a commonly used cell line in cancer research and drug development studies.

Automatically generated - may contain errors

Lab products found in correlation

Nci h520, by RIKEN BioResource Center (2 mentions) Mrc 5, by RIKEN BioResource Center (2 mentions) Mcf 7, by RIKEN BioResource Center (1 mentions) Helas3, by RIKEN BioResource Center (1 mentions) Hepg2, by RIKEN BioResource Center (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Wi 38, by RIKEN BioResource Center (1 mentions)

4 protocols using nci h460

Cancer Cell Line Cultivation and Maintenance

Check if the same lab product or an alternative is used in the 5 most similar protocols

The human cancer cell lines HeLaS3, KBvin, MCF-7, HepG2, NCI-H460 and NCI-H460/MX20 were grown in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (FBS) and 1/100 (v/v) penicillin/streptomycin at 37 °C under 5% carbon dioxide. The medium was routinely changed every two days. As cell confluence reached 80% in the medium, the cells were treated with 0.25% of trypsin-EDTA for the next passage. Human cervical carcinoma cell line HeLaS3, human non-small-cell lung carcinoma cell line NCI-H460, human liver carcinoma cell line HepG2 and human breast cancer cell line MCF7 were purchased from the Bioresource Collection and Research Center (Hsinchu, Taiwan). The multidrug-resistant human cervical cancer cell line KB-vin was kindly provided by Dr. Kuo-Hsiung Lee (University of North Carolina, Chapel Hill, NC, USA) and maintained with 100 nM of vincristine each week. MDR human non-small-cell lung carcinoma cell line NCI-H460/MX20 was the resistant cell line selected from NCI-H460. It was treated with gradually increasing concentrations of mitoxantrone and maintained in 20 nM of mitoxantrone.
Note: all tested compounds were dissolved in DMSO and the final concentrations of DMSO in all experiments were less than 0.1%.

Chen C.Y., Lien J.C., Chen C.Y., Hung C.C, & Lin H.C. (2021). Design, Synthesis and Evaluation of Novel Derivatives of Curcuminoids with Cytotoxicity. International Journal of Molecular Sciences, 22(22), 12171.

+ Open protocol

+ Expand

Cell Culture Conditions for Lung Cancer

Check if the same lab product or an alternative is used in the 5 most similar protocols

Normal lung cells (WI-38, MRC-5, HEL-299 cells lines), NSCLC (A549, NCI-H209, NCI-H460, NCI-H520 cell lines) and SCLC (NCI-H146 cell line) cells were purchased from the Bioresource Collection and Research Center (BCRC, Taiwan). They were cultured and grown at 37°C, in a humidified atmosphere with 5% CO₂, in a medium containing RPMI 1640 (Gibco, CA, USA), 2 mM L-glutamine, 10 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid), 1 mM sodium pyruvate, 4.5 g/l glucose, 1.5 g/l sodium bicarbonate and 10% fetal bovine serum (FBS, Gibco, CA, USA). Cell growth curves (number of cells versus time) were derived and when the cells were used in our experiments it is when they were at their log phases.

Huang C.Y., Huang H.Y., Forrest M.D., Pan Y.R., Wu W.J, & Chen H.M. (2014). Inhibition Effect of a Custom Peptide on Lung Tumors. PLoS ONE, 9(10), e109174.

+ Open protocol

+ Expand

Cell Line Culture Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols

A549, HOS, U2OS, NCI-H460 and NCI-H450 cell lines were obtained from Bioresource Collection and Research Center (BCRC, Hsinchu, Taiwan). Cells were cultured and maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) at 37 °C in 5% CO₂ humidified air.

Chiou W.C., Huang C., Lin Z.J., Hong L.S., Lai Y.H., Chen J.C, & Huang H.C. (2022). α-Viniferin and ε-Viniferin Inhibited TGF-β1-Induced Epithelial-Mesenchymal Transition, Migration and Invasion in Lung Cancer Cells through Downregulation of Vimentin Expression. Nutrients, 14(11), 2294.

+ Open protocol

+ Expand

Characterization of Lung Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

The human fetal lung fibroblast cell line MRC-5, human large cell lung carcinoma cell line NCI-H460 (H460) and human lung squamous cell carcinoma cell line NCI-H520 (H520) were obtained from Bioresource Collection and Research Center (BCRC) in Taiwan. BCRC has performed short tandem repeat polymerase chain reaction (STR-PCR) profile and confirmed the cells’ identity.
MRC-5 cells were cultured in 90% Eagle’s minimum essential medium supplemented with 10% fetal bovine serum (FBS), 0.1 mM non-essential amino acids and 1.0 mM sodium pyruvate. H460 and H520 cells were cultured in 90% RPMI 1640 medium with 10% fetal bovine serum. All media and reagents for cell culture were purchased from GIBCO (Thermo Fisher Scientific Inc., USA).
The cells were incubated in tissue culture polystyrene (TCPS) flasks (Nunc, Roskilde, Denmark), placed in an incubator filled with 5% CO₂ atmosphere and maintained at 37 °C. Cells were subcultured every 3–4 days. The cells used in the present study were within 10 to 15 passages. The cultured cells were routinely tested for mycoplasma using a commercial PCR kit (e-Myco plus, iNtRON Biotech, Korea). All the cells used in the present study were free of mycoplasma contamination.

Chang H.F., Cheng H.T., Chen H.Y., Yeung W.K, & Cheng J.Y. (2019). Doxycycline inhibits electric field-induced migration of non-small cell lung cancer (NSCLC) cells. Scientific Reports, 9, 8094.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!