Spr 67 nr

Invasive hemodynamic measurements were performed eight weeks after TAC or after sham operation as described [47 (link)]. The mice were anesthetized by the intraperitoneal administration of 1.4 g/kg urethane (Sigma, Steinheim, Germany). Body temperature was maintained with a heating pad and monitored with a rectal probe. An incision in the right carotid artery was made with a 26-gauge needle between a distal and proximal non-occlusive ligation of the artery. A 1.0 French Millar pressure catheter (SPR-67/NR; Millar instruments, Houston, TX, USA) was inserted and advanced to the left ventricle (LV). After the stabilization of the catheter, the heart rate, maximal systolic LV pressure, minimal diastolic LV pressure, peak rate of isovolumetric LV contraction (dP/dt_max), and peak rate of isovolumetric LV relaxation (dP/dt_min) were measured. The end-diastolic LV pressure was calculated manually from the pressure in the function of time curves. The time constant of isovolumetric LV pressure fall (tau) was calculated using the method of Weiss et al. [48 (link)]. Arterial blood pressure measurements were obtained after the withdrawal of the catheter from the LV to the ascending aorta. Data were registered with Powerlab Bridge Amplifier and Chart software (sampling rate of 2000 Hz; ADInstruments Ltd., Oxford, UK).

Invasive hemodynamic measurements were performed 8 weeks after TAC or after sham operation as described [12 (link), 43 (link), 44 (link)]. Mice were anesthetized by intraperitoneal administration of 1.4 g/kg urethane (Sigma, Steinheim, Germany). Body temperature was maintained with a heating pad and monitored with a rectal probe. An incision in the right carotid artery was made with a 26-gauge needle between a distal and proximal non-occlusive ligation of the artery. A 1.0 French Millar pressure catheter (SPR-67/NR; Millar instruments, Houston, Texas, USA) was inserted and advanced to the left ventricle (LV). After stabilisation of the catheter, heart rate, maximal systolic LV pressure, minimal diastolic LV pressure, the peak rate of isovolumetric LV contraction (dP/dt_max), and the peak rate of isovolumetric LV relaxation (dP/dt_min) were measured. The end-diastolic LV pressure was calculated manually from the pressure in function of time curves. The time constant of isovolumetric LV pressure fall (tau) was calculated using the method of Weiss et al. [45 (link)]. Arterial blood pressure measurements were obtained after withdrawal of the catheter from the LV to the ascending aorta. Data were registered with Powerlab Bridge Amplifier and Chart Software (sampling rate 2000 Hz; ADInstruments Ltd, Oxford, United Kingdom).

Invasive hemodynamic measurements were performed eight weeks after TAC or after sham operation. Mice were anesthetized by intraperitoneal administration of 1.4 g/kg urethane (Sigma, Steinheim, Germany). Body temperature was maintained with a heating pad and monitored with a rectal probe. An incision in the right carotid artery was made with a 26-gauge needle between a distal and proximal non-occlusive ligation of the artery. A 1.0 French Millar pressure catheter (SPR-67/NR; Millar instruments, Houston, TX, USA) was inserted and advanced to the left ventricle (LV). After stabilisation of the catheter, heart rate, maximal systolic LV pressure, minimal diastolic LV pressure, the peak rate of isovolumetric LV contraction (dP/dt_max), and the peak rate of isovolumetric LV relaxation (dP/dt_min) were measured. The end-diastolic LV pressure was calculated manually from the pressure in function of time curves. The time constant of isovolumetric LV pressure fall (tau) was calculated using the method of Weiss et al. [30 (link)]. Arterial blood pressure measurements were obtained after withdrawal of the catheter from the LV to the ascending aorta. Data were registered with Powerlab Bridge Amplifier and Chart Software (sampling rate 2000 Hz; ADInstruments Ltd., Oxford, UK).