Ish iview blue plus detection kit

Manufactured by Roche

Sourced in United States

The ISH iVIEW Blue Plus Detection Kit is a laboratory equipment product used for in-situ hybridization techniques. It provides a visual detection system for the identification and localization of specific nucleic acid sequences within cells or tissue samples.

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Lab products found in correlation

Eber 1 dnp probe, by Roche (2 mentions) Bx61vs microscope, by Olympus (1 mentions) Benchmark ultra automated ihc ish slide staining system, by Roche (1 mentions) Ventana benchmark ultra, by Roche (1 mentions) Red counterstain 2, by Roche (1 mentions) Alu positive control probe 2, by Roche (1 mentions) Ventana benchmark xt system, by Roche (1 mentions) Bond polymer refine detection system, by Leica (1 mentions) Inform hpv 3 family 16 probe, by Roche (1 mentions) Inform hpv 2 family 6 probe, by Roche (1 mentions)

Spelling variants of this product

ISH iVIEW Blue detection kit (8 citations) ISH iView kit (4 citations) IVIEW Plus Detection Kit (2 citations) Ventana ISH iView Blue Plus detection kit (2 citations)

9 protocols using ish iview blue plus detection kit

In situ Alu Sequence Detection

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In situ hybridization to detect human Alu repeat sequences was performed on paraffin‐embedded sections (N =2–3) using an ISH iVIEW Blue Plus Detection Kit (Ventana) and a BenchMark ULTRA automated IHC/ISH slide staining system (Ventana). According to the manufacturer's instructions, the Alu Positive Control Probe II was loaded with reagents from the detection kit and accessory reagents including Red Counterstain II onto the reagent tray and placed on the BenchMark ULTRA staining system. The slides were then loaded and the staining run was initiated. Human and rat tissues were used as positive and negative controls, respectively. After staining completed, the slides were dehydrated following the manufacturer's Dehydration Protocol. The slides were mounted with Surgipath MM 24 Mounting Medium (Leica Biosystems). All slides were imaged using an Olympus BX61VS microscope (Olympus Corporation, Tokyo, Japan, www.olympusamerica.com) with a Pike F‐505 camera (Allied Vision Technologies, Exton, PA, www.alliedvision.com).

Dang P.N., Herberg S., Varghai D., Riazi H., Varghai D., McMillan A., Awadallah A., Phillips L.M., Jeon O., Nguyen M.K., Dwivedi N., Yu X., Murphy W.L, & Alsberg E. (2017). Endochondral Ossification in Critical‐Sized Bone Defects via Readily Implantable Scaffold‐Free Stem Cell Constructs. Stem Cells Translational Medicine, 6(7), 1644-1659.

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Detection of EBV-infected Tumors by EBER CISH

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For detection of Epstein-Barr virus (EBV)-infected tumours, the presence of EBV-encoded RNA (EBER) transcripts was analysed by chromogenic in situ hybridization on 4-μm-thick TMA sections with appropriate controls, performed on the Ventana Benchmark ULTRA automated platform (Ventana Medical Systems, Tucson, AZ, USA) following the manufacturer's protocol. The EBER 1 DNP probe (760-1209, Ventana Medical Systems) was used for detection, ISH iVIEW Blue Plus Detection kit (760-097, Ventana Medical Systems) was used to produce the chromogenic reaction, and slides were counterstained with Red Counterstain II (780-2218, Ventana Medical Systems).

Svensson M.C., Borg D., Zhang C., Hedner C., Nodin B., Uhlén M., Mardinoglu A., Leandersson K, & Jirström K. (2019). Expression of PD-L1 and PD-1 in Chemoradiotherapy-Naïve Esophageal and Gastric Adenocarcinoma: Relationship With Mismatch Repair Status and Survival. Frontiers in Oncology, 9, 136.

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BOEC Incorporation and Biodistribution

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Adductor and gastrocnemius muscles, as well as lung, spleen, liver, kidney and heart on a separate set of animals not perfused with gelatin/barium‐sulfate were collected and paraffin‐embedded to study BOEC incorporation and biodistribution. BOEC‐incorporation into the vasculature of adductor and gastrocnemius muscles, was studied by combined chromogenic in situ hybridization for Arthrobacter luteus (ALU)‐repeats (ALU Positive Control Probe‐II and ISHiVIEW Blue Plus Detection Kit; Ventana Medical Systems, Inc., Oro Valley, AZ), as the primate‐specific sequence, and dual immunofluorescence staining for human (h) CD31 (anti‐hCD31 IgG_1,κ, m0823; Dako, Carpinteria, CA) and BS‐I Lectin (L3759; Sigma‐Aldrich) on adjacent sections. An initial acute retention and biodistribution study was performed, 24 hours after intramuscular injections (6 days after ligation) of 250 000 BOECs labeled by lentiviral overexpression of Cherry fluorescent protein. Moreover, chronic engraftment was quantified at 21 days.

Dauwe D., Pelacho B., Wibowo A., Walravens A., Verdonck K., Gillijns H., Caluwe E., Pokreisz P., van Gastel N., Carmeliet G., Depypere M., Maes F., Vanden Driessche N., Droogne W., Van Cleemput J., Vanhaecke J., Prosper F., Verfaillie C., Luttun A, & Janssens S. (2016). Neovascularization Potential of Blood Outgrowth Endothelial Cells From Patients With Stable Ischemic Heart Failure Is Preserved. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 5(4), e002288.

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Chromogenic In-Situ HPV DNA Detection

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Chromogenic in-situ hybridization to detect HPV DNA was performed using the high-risk HPV family 16 probe cocktail from Ventana Medical Systems (including HPV genotypes 16, 18, 31, 33, 35, 39, 51, 52, 56, 58, and 66) on six of seven cases of IUP, all cases of INV-LG, and four of eight cases of INV-HG. One case of IUP and four INV-HG did not have sufficient tissue present for evaluation. HPV DNA signals were visualized following hybridization on 4µm thick FFPE sections using ISH I View Blue Plus detection kit (Ventana Medical Systems; Tucson, Arizona), with hematoxylinas counterstain. HPV positivity was defined as strong, dark blue dot-like staining. Cervical tissue with HPV related squamous carcinoma was used as a positive control.

McDaniel A.S., Zhai Y., Cho K., Dhanasekaran S.M., Montgomery J.S., Palapattu G., Siddiqui J., Morgan T., Alva A., Weizer A., Lee C., Chinnaiyan A.M., Quist M.J., Grasso C.S., Tomlins S.A, & Mehra R. (2014). HRAS mutations are frequent in inverted urothelial neoplasms. Human pathology, 45(9), 1957-1965.

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HPV Detection in FFPE Tissues

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DNA ISH was performed on formalin-fixed, paraffin-embedded (FFPE) tissue blocks from each case using the ISH I View Blue Plus Detection Kit (Ventana Medical System, Inc.) in accordance with the manufacturer's instructions. The assay used the Ventana HPV III Family 16, Probe B, a cocktail recognizing the HPV types 16, 18, 31, 33, 35, 45, 51, 52, 56, 59, 68 and 70. Ventana Red Counterstain II (Ventana Medical System, Inc.) was used (Fig. 1).
Controls in each run included a known HPV 16-positive HeLa cell line. A pathologist read the cases, and blue nuclear dots were considered positive staining. Any definitive nuclear staining in the tumor cells was considered positive. Cases were classified in a binary manner as either positive or negative.

Fukahori M., Kato K., Taniguchi H., Ohtomo R., Takahashi N., Shoji H., Iwasa S., Honma Y., Takashima A., Hamaguchi T., Yamada Y., Shimada Y., Ito Y., Itami J., Hokamura N., Igaki H., Tachimori Y., Miwa K., Torimura T, & Boku N. (2020). Relationship between cervical esophageal squamous cell carcinoma and human papilloma virus infection and gene mutations. Molecular and Clinical Oncology, 14(2), 41.

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Detecting HPV DNA in Small Cell Carcinoma

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Three-micron sections containing small cell carcinoma and other types of epithelial tumors were examined for HPV DNA by ISH staining with INFORM® HPV III Family 16 and 6 Probe (Ventana Medical Systems, Inc., Tucson, AZ, USA) for high-risk HPV types (16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, and 66) and low-risk HPV types (6 and 11), respectively. The ISH assay was performed according to the manufacturer’s protocol using the Ventana BenchMark XT system (Ventana Medical Systems, Inc.). Labeling was detected with the ISH iVIEW™ Blue Plus Detection Kit (Ventana Medical Systems, Inc.). The positive and negative controls were carried out using HPV quality control slides provided by Ventana Medical Systems, Inc. The HPV signals were detected in the nuclei of tumor cells, and the signal patterns were categorized as either episomal staining pattern or punctuate staining pattern. The former presents as a homogeneous globular navy-blue to black signal in the entire nuclei of tumor cells, and the latter shows single or multiple sparsely distributed and dot-like navy-blue punctae in the nuclei of the tumor cells.

Li P., Ma J., Zhang X., Guo Y., Liu Y., Li X., Zhao D, & Wang Z. (2018). Cervical small cell carcinoma frequently presented in multiple high risk HPV infection and often associated with other type of epithelial tumors. Diagnostic Pathology, 13, 31.

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Immunohistochemical Analysis of FFPE Tissues

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For morphological analyses, sample tissues were formalin-fixed, paraffin-embedded (FFPE), sliced into 4-μm sections, and subjected to standard hematoxylin and eosin (H&E) staining or immunohistochemistry (IHC). IHC was performed using the Bond Polymer Refine Detection system (Leica Microsystems, Tokyo, Japan) according to the manufacturer’s instructions. Nuclei were counterstained with hematoxylin. Primary antibodies used for IHC were: monoclonal anti-HLA class 1-A, B, C (Hokudo, Sapporo, Japan), rabbit polyclonal anti-c-kit (Nichirei Biosciences, Tokyo, Japan); monoclonal anti-CD34, clone NU-4A1 (Nichirei Biosciences); monoclonal antileukocyte common antigen, clone PD7/26, 2B11 (CD111, Nichirei Biosciences); HER2 (Hercep Test™, Dako, Japan), monoclonal anti-estrogen receptor (ER), clone 1D5 (Nichirei Biosciences); and monoclonal antiprogesterone receptor (PgR), clone A9621A (Nichirei Biosciences). Chromogenic in situ hybridization (ISH) for Epstein-Barr virus (EBV)-encoded RNA (EBER) was performed using the EBER 1 DNP probe (Ventana/Roche, Tuscon, AZ, USA) and the ISH iView blue plus detection kit (Ventana/Roche) according to the provider’s instructions.

CHIJIWA T., KAWAI K., NOGUCHI A., SATO H., HAYASHI A., CHO H., SHIOZAWA M., KISHIDA T., MORINAGA S., YOKOSE T., KATAYAMA M., TAKENAKA N., SUEMIZU H., YAMADA R., NAKAMURA Y., OHTSU T., TAKANO Y., IMAI K., MIYAGI Y, & NAKAMURA M. (2015). Establishment of patient-derived cancer xenografts in immunodeficient NOG mice. International Journal of Oncology, 47(1), 61-70.

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HPV Detection in Head and Neck Tumors

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HPV DNA analysis also was performed on tissue specimens from patients who had undergone resections of their head and neck tumors. Briefly, five micron FFPE tissue sections from resected tumors or biopsies were conditioned with Ventana cell conditioner #2 and ISH-protease 3. Hybridization was performed using the HPV III Family16 probe set that captures the HR-HPV genotypes 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58 and 66. The fluorescein-labeled probes that identify the HR genotypes were detected with the ISH iVIEW Blue Plus Detection Kit (Ventana Medical Systems, Inc., Tucson, AZ). Punctate hybridization signals localized to the tumor cell nuclei defined an HPV-associated tumor.

Smith D.F., Maleki Z., Coughlan D., Gooi Z., Akpeng B., Ogawa T., Bishop J.A., Frick K.D., Agrawal N., Gourin C.G., Ha P.K., Koch W.M., Richmon J.D., Westra W.H, & Pai S.I. (2014). Human papillomavirus status of head and neck cancer as determined in cytologic specimens using the hybrid-capture 2 assay. Oral oncology, 50(6), 600-604.

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In-Situ Hybridization for HPV Subtypes

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ISH was performed with INFORM-HPVII-Family6 probe and INFORM-HPVIII-Family16 probe (Ventana-Medical-System) with an ISH iVIEW Blue Plus Detection Kit (Ventana-Medical-System) on a Ventana Benchmark Ultra in accordance with the manufacturer's instructions. A positive control was included on each slide.

Frouin E., Guillot B., Larrieux M., Tempier A., Boulle N., Foulongne V., Girard C., Costes V, & Solassol J. (2014). Cutaneous Epithelial Tumors Induced by Vemurafenib Involve the MAPK and Pi3KCA Pathways but Not HPV nor HPyV Viral Infection. PLoS ONE, 9(10), e110478.

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