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3 protocols using penicillin

1

Cytotoxicity Assay Protocol for Chrysanthemin

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Chrysanthemin (≥ 95% purity as determined by HPLC), MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), dimethyl sulfoxide, 5% heat-inactivated fetal calf serum, penicillin, streptomycin were purchased from Thomas Scientific, High Hill Road, Swedesboro, U.S.A. Acridine orange (AO)/ethidium bromide (ETBR), propidium iodide and rhodamine-123 were purchased from Wuhan Boster Biological Technology Ltd. (Wuhan, China).
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2

Cytotoxicity Evaluation of Methylwogonin

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Methylwogonin (≥ 95%), MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), and dimethyl sulfoxide were obtained from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Acridine orange, propidium iodide and Hoechst 33342 were procured from Wuhan Boster Biological Technology Ltd. (Wuhan, China). Dulbecco’s modified Eagle’s medium and RPMI-1640 medium were obtained from Gibco Life Technologies, Grand Island, NY, USA). Heat-inactivated fetal calf serum, penicillin, and streptomycin were obtained from Thomas Scientific, High Hill Road, Swedesboro, U.S.A.
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3

Cytotoxicity Assay Protocol

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5-Methoxypsoralen (≥ 95% by HPLC), MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), and dimethyl sulfoxide were obtained from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Acridine orange and propidium iodide were procured from Wuhan Boster Biological Technology Ltd. (Wuhan, China). Dulbecco’s modified Eagle’s medium and RPMI-1640 medium were obtained from Gibco Life Technologies (Grand Island, NY, USA). Heat-inactivated fetal calf serum, penicillin, and streptomycin were obtained from Thomas Scientific, High Hill Road, Swedesboro, U.S.A.
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