The spleens of mice in each experimental group were aseptically collected, and splenic cell suspension was prepared. The proliferation of CD4+ T cells and the ratio of Th1/Th2 in spleen cell suspension were detected according to the reported method [20 (link)]. In brief, the Th1/Th2 ratio in the splenic cell suspension was detected by the Th1/Th2 flow cytometry kit (Lianke Biotech, No. 70-KTH201) according to the kit’s instructions. Cell suspension was incubated for one hour at RT in the dark after adding Anti-mouse CD4-FITC antibody (Invitrogen, No. 11-0042-85) and Brdu-PE antibody (Invitrogen, No. 12-5071-41), respectively, and then determined by flow cytometry.
Anti mouse cd4 fitc antibody
Manufactured by Thermo Fisher Scientific
The Anti-mouse CD4-FITC antibody is a fluorescently-labeled monoclonal antibody that specifically binds to the CD4 surface antigen expressed on mouse T helper cells. It is designed for use in flow cytometry applications to identify and quantify CD4-positive cell populations.
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Lab products found in correlation
2 protocols using anti mouse cd4 fitc antibody
1
Murine Splenic T Cell Profiling
2
Quantification of Th1/Th2 Ratio in Murine Splenocytes
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The spleens of mice in each experimental group were aseptically collected, and splenic cell suspension was prepared. The proliferation of CD4 + T cells and the ratio of Th1/Th2 in spleen cell suspension were detected according to the reported method [20] . In brief, the Th1/Th2 ratio in the splenic cell suspension was detected by the Th1/Th2 ow cytometry kit (Lianke Biotech, No. 70-KTH201) according to the kit's instructions. Cell suspension was incubated for one hour at RT in the dark after adding Anti-mouse CD4-FITC antibody (Invitrogen, No. 11-0042-85) and Brdu-PE antibody (Invitrogen, No. 12-5071-41), respectively, and then determined by ow cytometry.
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