BALF supernatant collection was performed based on the protocol of Van Hoecke et al. (2017) (link). BALFs were collected by tracheal infusions of 1 mL phosphate-buffered saline (PBS), and perfused BALFs were centrifuged for 4 min at 700 × g. The supernatants were harvested and stored at −80°C until analysis. The protein levels of IL-4 and IL-5 were measured using the ELISA MAX™ Deluxe Set Mouse IL-4 and IL-5 (BioLegend, Inc., San Diego, CA, United States) according to the manufacturer’s protocol.
Elisa max deluxe set mouse il 4 and il 5
Manufactured by BioLegend
Sourced in United States
ELISA MAX™ Deluxe Set Mouse IL-4 and IL-5 is a sandwich enzyme-linked immunosorbent assay (ELISA) kit for the quantitative measurement of mouse interleukin-4 (IL-4) and interleukin-5 (IL-5) in cell culture supernatants, serum, and plasma.
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Lab products found in correlation
2 protocols using elisa max deluxe set mouse il 4 and il 5
1
BALF Supernatant Collection and Cytokine Analysis
2
Quantification of Immune Mediators
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The protein levels of IL-4 and IL-5 in BALF were measured by using ELISA MAX™ Deluxe Set Mouse IL-4 and IL-5 (BioLegend, USA) according to the manufacturer’s protocol. For serum IgE analysis, the obtained serum was stored at − 80 °C and measured using ELISA MAX™ Deluxe Set Mouse IgE (BioLegend) according to the manufacturer’s instructions.
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