Elite abc peroxidase kit

Manufactured by Vector Laboratories

Sourced in United States

The Elite ABC Peroxidase Kit is a laboratory product designed for immunohistochemistry and other applications. It provides a reliable and consistent method for the detection of target antigens in tissue samples.

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Lab products found in correlation

Dab substrate, by Vector Laboratories (2 mentions) Avidin biotin blocking kit, by Vector Laboratories (1 mentions) Anti fasn, by Cell Signaling Technology (1 mentions) Biocytin, by Merck Group (1 mentions) Biocytin, by Vector Laboratories (1 mentions) Sirt1, by Merck Group (1 mentions) Goat anti mouse igg hrp, by Cell Signaling Technology (1 mentions) Hrp linked goat anti rabbit igg, by Cell Signaling Technology (1 mentions) Rabbit anti tyrosine hydroxylase th, by Abcam (1 mentions) Mouse anti neuronal nuclei neun, by Abcam (1 mentions) Rabbit anti caspase 3, by Cell Signaling Technology (1 mentions) Fabp4, by Cell Signaling Technology (1 mentions) Alexa 488, by Thermo Fisher Scientific (1 mentions) Alexa 588, by Thermo Fisher Scientific (1 mentions) Lyve 1, by Abcam (1 mentions) Pan cytokeratin, by Agilent Technologies (1 mentions) Triton x 100, by Merck Group (1 mentions) Biotinylated secondary antibody, by Vector Laboratories (1 mentions) Citrisolv, by Thermo Fisher Scientific (1 mentions) Immpact dab substrate, by Vector Laboratories (1 mentions)

8 protocols using elite abc peroxidase kit

Immunohistochemical Analysis of Liver Lipogenesis

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Upon euthanasia, mouse liver tissues were collected and fixed in 4% paraformaldehyde (PFA) overnight at 4°C and embedded in paraffin. For antigen retrieval, slides were put into 10 mM sodium citrate buffer (pH 6.0), and placed in a microwave on high for 10 min. Slides were blocked with 5% goat serum and the Avidin-Biotin blocking kit (Vector Laboratories, Burlingame, CA). Primary antibodies were added to the slides and incubated overnight at 4°C. Slides were washed and incubated with a biotin conjugated secondary antibody. Detection was performed with the ABC-Elite peroxidase kit (Vector Laboratories) using the DAB as the substrate. Primary antibodies used in the study include: Anti-FASN and anti-acetyl-CoA carboxylase (anti-ACC) antibodies; both were obtained from Cell Signaling Technology Inc.

Li L., Che L., Wang C., Blecha J.E., Li X., VanBrocklin H.F., Calvisi D.F., Puchowicz M., Chen X, & Seo Y. (2016). [11C]acetate PET Imaging is not Always Associated with Increased Lipogenesis in Hepatocellular Carcinoma in Mice. Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging, 18(3), 360-367.

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Intracellular Biocytin Labeling of MSNs

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In a subset of experiments, intracellular glass micropipettes were filled with 1.5% biocytin (Sigma), dissolved in 2 M potassium acetate. MSNs were passively filled during at least 80 min of recordings. At the end of the electrophysiological experiment, rats were deeply anaesthetised (sodium pentobarbital, 150 mg⁄kg i.p.) and transcardially perfused with saline followed by 4% ice-cold paraformaldehyde in 0.1 M phosphate buffer. Brains were extracted and, following 2 h in the fixative solution, were transferred to 30% sucrose. Brains were then cut in frontal 50-µm sections using a freezing microtome and slices were maintained in 0.1 M potassium-PBS (pH = 7.4). To recover the recorded cell and visualize the biocytin-filled neurons, avidin-biotin-horseradish peroxidase was used (ABC Elite peroxidase kit; Vector Laboratories, Burlingame, CA, USA) according to manufacturer’s instructions.

Morera-Herreras T., Gioanni Y., Perez S., Vignoud G, & Venance L. (2019). Environmental enrichment shapes striatal spike-timing-dependent plasticity in vivo. Scientific Reports, 9, 19451.

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SIRT1 Immunohistochemical Analysis

Cited 1 time

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IHC was performed on formalin-fixed, paraffin-embedded tissue sections with sodium citrate antigen retrieval, followed by visualization with the ABC Elite peroxidase kit and DAB substrate (Vector Labs) for detection of SIRT1 (Millipore 04-1557, 1:100). IHC results were semiquantitatively analysed using the Allred Score17 (link).

Sedic M., Skibinski A., Brown N., Gallardo M., Mulligan P., Martinez P., Keller P.J., Glover E., Richardson A.L., Cowan J., Toland A.E., Ravichandran K., Riethman H., Naber S.P., Näär A.M., Blasco M.A., Hinds P.W, & Kuperwasser C. (2015). Haploinsufficiency for BRCA1 leads to cell-type-specific genomic instability and premature senescence. Nature Communications, 6, 7505.

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Immunohistochemical and ELISA Analysis in MPTP-induced Parkinson's Model

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MPTP was purchased from Sigma-Aldrich, Canada (Oakville, ON, Canada). Rabbit anti-tyrosine hydroxylase (TH; Cat# ab6211, RRID: AB_2240393) and mouse anti-neuronal nuclei (NeuN; Cat# MAB377, RRID: AB_2298772) were obtained from Abcam (Toronto, ON, Canada), and MilliporeSigma (Oakville, ON, Canada), respectively; rabbit anti-ionized calcium-binding adapter molecule 1 (IBA1; Cat# MA5-36257, RRID: AB_2890455) and mouse anti-glial fibrillary acidic protein (GFAP; Cat# MA5-12023, RRID: AB_10984338) were purchased from Invitrogen (Burlington, ON, Canada); rabbit anti-caspase-3 (Cat# 9662S, RRID: AB_331439), goat anti-mouse IgG (HRP linked; Cat# 7076S, RRID: AB_330924), and goat anti-rabbit IgG (HRP linked; Cat# 7074S, RRID: AB_2099233) were purchased from Cell Signaling (Beverly, MA, USA); goat biotinylated anti-mouse IgG (H&L; Cat# BA-9200, RRID: AB_2336171), goat biotinylated anti-rabbit IgG (H&L; Cat# BA-1000, RRID: AB_2313606), and the elite ABC-peroxidase kit (Cat# PK-6100, RRID: AB_2336819) were purchased from Vector Laboratories (Newark, CA, USA); mouse Anti-ß actin (C4) HRP (Cat# sc-47778 HRP, RRID: AB_2714189) was purchased from Santa Cruz Biotechnology (Dallas, TX, USA); and the dopamine (DA) ELISA kit (Cat# RD-DA-Ge) was obtained from Reddot Biotech Inc. (Kelowna, BC, Canada). All other chemicals and reagents used were of analytical grade.

Sekar S., Zhang Y., Miranzadeh Mahabadi H., Buettner B, & Taghibiglou C. (2023). Low-Field Magnetic Stimulation Alleviates MPTP-Induced Alterations in Motor Function and Dopaminergic Neurons in Male Mice. International Journal of Molecular Sciences, 24(12), 10328.

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Immunohistochemistry and Immunofluorescence Analysis

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LYVE-1 (1:100; Abcam, Cambridge, MA, USA) immunohistochemistry was performed on formalin-fixed, paraffin-embedded tissue sections with sodium citrate antigen retrieval, followed by detection with biotinylated secondary antibody (1:200 anti-rabbit; Vector Laboratories) and visualization with the Elite ABC Peroxidase Kit and DAB substrate (Vector Laboratories, Burlingame, CA, USA). Nuclei were counterstained for hematoxylin. Immunofluorescence on frozen tissue or cultured cells was performed on samples that were permeabilized with 0.1% Triton X-100 (Sigma) in PBS, washed and blocked in 1% BSA in PBS at ambient temperature. Samples were incubated with cellular retinoic acid binding protein-1 (crabp1) (1:250, Abcam), CD31 (1:100, Invitrogen, Carlsbad, CA, USA), pan-cytokeratin (1:100, DAKO, Carpinteria, CA, USA)) or FABP4 (1:200, Cell Signaling Technology, Danvers, MA, USA) overnight at 4°C. The fluorescence signal was detected using secondary antibodies (1:500 conjugated Alexa488 and Alexa588, Invitrogen). Nuclei were stained with 4′,6- diamidino-2-phynylindole (DAPI) and images were captured with the Spot imaging software system (Diagnostic Instruments, Inc.). Quantification was performed using ImageJ software. Immunofluorescence on frozen tissue sections was performed as previously described for crabp1 [32 (link)].

McCready J., Arendt L.M., Glover E., Iyer V., Briendel J.L., Lyle S.R., Naber S.P., Jay D.G, & Kuperwasser C. (2014). Pregnancy-associated breast cancers are driven by differences in adipose stromal cells present during lactation. Breast Cancer Research : BCR, 16(1), R2.

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Quantifying Cell Proliferation in Snakes

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We examined potential differences in cell proliferation among snakes using BrdU immunohistochemistry methods identical to those described by Maine et al. (2014b (link)). Briefly, antigen retrieval was performed by incubating slides in 2 N HCl in 0.1 M phosphate-buffered saline (PBS; pH 7.4) at 37°C for 30 min to denature the DNA. BrdU immunoreactivity was examined using a rat anti-BrdU antiserum (OBT0030, Accurate Chemical, Westbury, NY, USA) at a dilution of 1:5,000 in PBS containing 0.3% Triton X and 10% normal goat serum. Sections were incubated with primary antibody for 48 h at 4°C in a humid chamber. Primary antibody signal was amplified by incubation with biotinylated goat anti-rat IgG secondary antibody (BA-9400, Vector Labs Inc., Burlingame, CA, USA) diluted 1:400 followed by avidin conjugated to horseradish peroxidase (Elite ABC peroxidase kit, Vector Labs, Inc.). Primary antibody binding was visualized using 0.25 mg/ml diaminobenzidine in 0.2% hydrogen peroxide in 0.05 M Tris buffer (pH 7.2). Tissues were counterstained for 1 min in hematoxylin, dehydrated in a graded ethanol series, cleared with Citrisolv (Fisher Scientific, Pittsburgh, PA, USA) and coverslipped.

Lutterschmidt D.I., Lucas A.R., Karam R.A., Nguyen V.T, & Rasmussen M.R. (2018). Sexually Dimorphic Patterns of Cell Proliferation in the Brain Are Linked to Seasonal Life-History Transitions in Red-Sided Garter Snakes. Frontiers in Neuroscience, 12, 364.

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Immunohistochemical Analysis of Pancreatic Tumors

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Pancreatic tumor sections were isolated from both PKT mice and orthotopic
xenografts and fixed in 10% neutral buffered formalin solution. Tissue slides
were then deparaffinized followed by heat induced antigen retrieval in citrate
buffer (pH = 6.0). This was followed by quenching endogenous peroxidase activity
by incubating in 3% H₂O₂ for ten minutes. Tissues were
further immunostained using indicated primary antibodies (Supplementary Table S1). Primary
antibodies were then detected using VECTASTAIN Elite ABC peroxidase kit as per
the manufacturer’s protocol using diaminobenzidine (DAB) as the
chromogen. Finally, the sections were counterstained with Mayer’s
hematoxylin and mounted with D.P.X. Tissue sections and were microscopically
examined. Digital slide images were adjusted to exclude areas containing
histologic artifacts, such as tissue folds or nonorganic material, from the
digital image. Positive staining was quantified by using ImageJ image analysis
software (NIH) and reported as percentage area of staining. Trichrome blue
staining was performed as previously described (14 (link)). PKT tumor tissues from four week endpoint analysis arm were
additionally stained using hematoxylin and eosin. Total tumor area relative to
normal pancreas was estimated in these sections by manual quantification of
random fields.

Dosch A.R., Dai X., Reyzer M.L., Mehra S., Srinivasan S., Willobee B.A., Kwon D., Kashikar N., Caprioli R., Merchant N.B, & Nagathihalli N.S. (2020). Combined Src/EGFR inhibition targets STAT3 signaling and induces stromal remodeling to improve survival in pancreatic cancer. Molecular cancer research : MCR, 18(4), 623-631.

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Immunohistochemical Analysis of γH2AX

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Mouse mammary tissue samples were fixed overnight in 10% neutral-buffered formalin, embedded in paraffin, and cut into sections for H&E or immunohistochemical staining. For immunohistochemical staining, tissue sections were de-paraffinized in xylenes and rehydrated using graded ethanols (100%, 95%, 70%) followed by peroxidase inactivation via incubation in 0.5% H 2 O 2 and antigen retrieval in citrate buffer (10 mM, pH 6) buffer for 30 min at 100 C. Samples were blocked in 1% bovine serum albumin and 1% goat serum in PBS for 1 hr at room temperature and incubated overnight at 4 C with primary anti-gH2AX antibody (1:400, Cell Signaling), followed by detection with biotinylated anti-rabbit secondary antibody (1:500, Vector), and visualization with Elite ABC Peroxidase Kit and ImmPact DAB substrate (Vector Laboratories). Samples were counterstained in hematoxylin followed by Cell Reports 28, 394-407.e1-e6, July 9, 2019 e4 dehydration in graded ethanols (70%, 95%, 100%) & xylenes and mounting using Permount (Fisher). Images were captured using the Eclipse 80i microscope and SPOT image acquisition software.

Gross K.M., Zhou W., Breindel J.L., Ouyang J., Jin D.X., Sokol E.S., Gupta P.B., Huber K., Zou L, & Kuperwasser C. (2019). Loss of Slug Compromises DNA Damage Repair and Accelerates Stem Cell Aging in Mammary Epithelium. Cell reports, 28(2).

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