Saponin was from Merck (Darmstadt, Germany), Zymozan A, albumin, DMSO and glycerol from Sigma (St. Louis, MO, USA), sterile endotoxin-free Hank’s balanced salt solution (HBSS) from Thermo Scientific (Waltham, MA, USA) and Ficoll from Phadia (Uppsala, Sweden). All other reagents were of research grade. HBSS contained 135 mM NaCl, 4 mM KCl, 1 mM MgCl2, 2 mM CaCl2, 10 mM glucose, 1 mM Na2HPO4, 20 mM HEPES and pH=7.3. Green fluorescent protein (GFP)-expressing Staphylococcus aureus was a kind gift from Professor William Nauseef (University of Iowa, IA, USA).
Zymozan a
Manufactured by Merck Group
Sourced in United States
Zymozan A is a laboratory enzyme product manufactured by Merck Group. It is a glycoside hydrolase enzyme that catalyzes the hydrolysis of beta-1,3-glucosidic linkages in various polysaccharides.
Automatically generated - may contain errors
Lab products found in correlation
Caffeine, by Thermo Fisher Scientific (2 mentions)
Ketoprofen, by Merck Group (2 mentions)
Evans blue, by Merck Group (2 mentions)
Albumin, by Merck Group (1 mentions)
Hanks balanced salt solution (hbss), by Thermo Fisher Scientific (1 mentions)
Glycerol, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Saponin, by Merck Group (1 mentions)
3 protocols using zymozan a
1
Phagocytosis of GFP-expressing Staphylococcus aureus
2
Anti-inflammatory Compound Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Ketoprofen was used as standard anti-inflammatory compound and was purchased from Sigma-Aldrich (Poland). Carrageen was purchased from FCM Corporation (USA), Zymozan A and Evans blue from Sigma-Aldrich (Poland), Caffeine (used as standard in obese model) was purchased from Alfa-Aesar (Poland). KD-64—(1,3-dimethyl-9-((1r,4r)-4-methylcyclohexyl-6,7,8,9-tetrahydropyrimido[2,1-f]purine-2,3(1H,3H)-dione) (Fig 1B ) [43 (link)] was synthesized in the Department of Technology and Biotechnology of Drugs, Faculty of Pharmacy, Jagiellonian University Medical College, Cracow, Poland. NMR and LC-MS techniques assessed identity and purity of final product. In all experiments Ketoprofen, Caffeine or KD-64 were administered as suspensions in 1% Tween 80 in sterile water for injection.
3
Anti-inflammatory Compound Evaluation Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Ketoprofen was used as standard anti-inflammatory compound and was purchased from Sigma-Aldrich (Poland). Carrageen was purchased from FCM Corporation (USA), Zymozan A and Evans blue from Sigma-Aldrich (Poland), Caffeine (used as standard in obese model) was purchased from Alfa-Aesar (Poland). KD-64 (Figure 1) [42]
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!