Total cholesterol (TC), HDL cholesterol (HDL-C), LDL cholesterol (LDL-C), triglycerides (TG), glucose, and liver enzymes (AST, aspartate aminotransferase; ALT, alanine aminotransferase) were measured via enzymatic colorimetric methods using an automatic analyzer (Siemens®, Erlangen, Germany) in a certified clinical laboratory facility.
Automatic analyzer
Manufactured by Siemens
Sourced in United States
The Automatic Analyzer is a laboratory equipment designed to perform automated analysis of various samples. It is capable of conducting precise and consistent measurements through the use of advanced sensors and software algorithms. The core function of the Automatic Analyzer is to automate the analytical processes, reducing the need for manual intervention and increasing the efficiency and accuracy of the laboratory operations.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using automatic analyzer
1
Metabolic Biomarker Measurement Protocol
2
Comprehensive Metabolic Profiling in Health
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Fasting blood lipids (total cholesterol, HDL-c, non-HDL-c, and TG) and glucose, and urinary creatinine were measured in an automatic analyzer (SIEMENS, Washington, DC, USA). LDL-c concentration was calculated using the Friedewald formula [43 ]. For the determination of insulin, a sandwich immunoassay was employed with direct chemiluminescence technology (SIEMENS, Washington, DC, USA) in an automated analyzer. Atherogenic indexes of plasma were calculated based on the literature [44 ,45 (link)].
3
Serum Lipid and Glucose Profiling
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Serum total cholesterol, HDL cholesterol, LDL cholesterol and triglycerides were measured with an automatic analyzer (Siemens Healthcare, Tarrytown, NY, USA). Plasma insulin was evaluated using a commercial ELISA kit (Merck-Millipore, Burlington, MS, USA). Fasting blood glucose was determined by applying the enzyme electrode method using a Free Style Optimum Neo blood glucose meter from Abbott (Chicago, IL, USA).
Blood pressure was measured in a quiet temperature-controlled room using an automated digital oscillometric device (Omron model M6 Comfort, Omron Corporation, Tokyo, Japan).
Height, body weight and abdominal and hip perimeter were also registered.
Blood pressure was measured in a quiet temperature-controlled room using an automated digital oscillometric device (Omron model M6 Comfort, Omron Corporation, Tokyo, Japan).
Height, body weight and abdominal and hip perimeter were also registered.
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