E. coli C41 (DE3) pLysS was obtained from Novagen. pET28a expression vector was purchased from Invitrogen. Fetal bovine serum (FBS) and Dulbecco’s Modified Eagle’s Medium F12 (DMEM F12) were purchased from Gibco, plasmid extraction kit and Ni-NTA-Sepharose column from Qiagen. IPTG obtained from Vivantis. Luciferin was purchased from Resem (the Netherlands). Adenosine triphosphate (ATP) and dialysis bag (2.5 KDa) were purchased from Sigma. MTT 3-(4,5-s-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide was obtained from Sigma–Aldrich. Graphite powder (mesh~200 µm), KMnO4, NaNO3, H2O2, Dimethylformamide (DMF) were purchased from Chemlab. Sulfuric acid (98%) was obtained from Merck.
Dulbecco s modified eagle s medium f12 dmem f12
Manufactured by Thermo Fisher Scientific
Sourced in United States, United Kingdom
Dulbecco's modified Eagle's medium/F12 (DMEM/F12) is a cell culture medium used to support the growth and maintenance of various cell types. It is a nutrient-rich formulation that provides essential components for cell proliferation and survival. DMEM/F12 is a widely used medium for a variety of applications in cell biology and tissue engineering.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (17 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (4 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (4 mentions)
Fetal bovine serum (fbs), by Merck Group (3 mentions)
B27 supplement, by Thermo Fisher Scientific (3 mentions)
Recombinant rat il 1β, by R&D Systems (2 mentions)
Beclin 1, by Cell Signaling Technology (2 mentions)
Foetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
High capacity cdna reverse transcription kit, by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin, by Merck Group (2 mentions)
Trizol, by Thermo Fisher Scientific (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Dulbecco phosphate buffered saline (dpbs), by Thermo Fisher Scientific (2 mentions)
L glutamine, by Thermo Fisher Scientific (2 mentions)
Trypsin, by Thermo Fisher Scientific (2 mentions)
Epidermal growth factor (egf), by Merck Group (2 mentions)
Insulin, by Merck Group (2 mentions)
Amphotericin b, by Merck Group (2 mentions)
Gentamicin, by Merck Group (2 mentions)
Pet28a expression vector, by Thermo Fisher Scientific (1 mentions)
38 protocols using dulbecco s modified eagle s medium f12 dmem f12
1
Recombinant Protein Expression in E. coli
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E. coli C41 (DE3) pLysS was obtained from Novagen. pET28a expression vector was purchased from Invitrogen. Fetal bovine serum (FBS) and Dulbecco’s Modified Eagle’s Medium F12 (DMEM F12) were purchased from Gibco, plasmid extraction kit and Ni-NTA-Sepharose column from Qiagen. IPTG obtained from Vivantis. Luciferin was purchased from Resem (the Netherlands). Adenosine triphosphate (ATP) and dialysis bag (2.5 KDa) were purchased from Sigma. MTT 3-(4,5-s-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide was obtained from Sigma–Aldrich. Graphite powder (mesh~200 µm), KMnO4, NaNO3, H2O2, Dimethylformamide (DMF) were purchased from Chemlab. Sulfuric acid (98%) was obtained from Merck.
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E. coli C41 (DE3) pLysS was obtained from Novagen. pET28a expression vector was purchased from Invitrogen. Fetal bovine serum (FBS) and Dulbecco’s Modified Eagle’s Medium F12 (DMEM F12) were purchased from Gibco, plasmid extraction kit and Ni-NTA-Sepharose column from Qiagen. IPTG obtained from Vivantis. Luciferin was purchased from Resem (the Netherlands). Adenosine triphosphate (ATP) and dialysis bag (2.5 KDa) were purchased from Sigma. MTT 3-(4,5-s-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide was obtained from Sigma–Aldrich. Graphite powder (mesh~200 µm), KMnO4, NaNO3, H2O2, Dimethylformamide (DMF) were purchased from Chemlab. Sulfuric acid (98%) was obtained from Merck.
2
Evaluation of Neuroprotective Effects
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AS-IV (Fig. 1 ), with a purity >98%, was obtained from the Nanjing Zelang Medical Technology Co., Ltd. (Nanjing, China). Bovine serum albumin (BSA), 3-(4,5-dimethylthiazol-2-yl)-2,5-dephenyltetrazolium bromide (MTT), dimethyl sulfoxide (DMSO), 2′,7′-dichlorofluorescein diacetate (DCFH-DA), vitamin C (Vit C) and H2O2 were all purchased from Sigma-Aldrich (St. Louis, MO, USA). The Annexin V-fluorescein isothiocyanate (FITC) apoptosis detection kit was obtained from KeyGen Biotech Co., Ltd. (Nanjing, China). Fetal bovine serum (FBS) and Dulbecco's modified Eagle's medium/F12 (DMEM/F12) were both purchased from Gibco (Grand Island, NY, USA). Anti-α-synuclein antibody (#ab138501) was purchased from Epitomics (Burlingame, CA, USA). Anti-β-actin (#3700), anti-Bcl-2 (#15071) and anti-Bax (#5023) monoclonal antibodies were all purchased from Cell Signalling Technology, Inc. (Beverly, MA, USA). Anti-p38 mitogen-activated protein kinase (MAPK; sc-7972), anti-p-p38 (sc-17852-R), anti-p-JNK (sc-293136), anti-ERK1/2 (sc-514302), anti-p-ERK1/2 (sc-16981-R) and anti-TH (sc-7847) mono clonal antibodies were all purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). All of the other reagents were of analytical grade.
3
Autophagy Regulation in Cellular Processes
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Fetal bovine serum (FBS) and Dulbecco's modified Eagle's medium/F12 (DMEM/F12) were purchased from Gibco (Grand Island, NY, USA). AGE-bovine serum albumin (BSA) was purchased from Merck Millipore (Darmstadt, Germany). BSA and the inhibitor of autophagy 3-methyladenine (3-MA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). The inducer of autophagy rapamycin (RA) was purchased from Selleck Chemicals (Houston, TX, USA). Cell Counting Kit-8 (CCK-8) was obtained from Zoman Biotech. Co., Ltd. (Beijing, China). The Annexin V-FITC/PI apoptosis detection kit was purchased from Multi Sciences Biotech. Co., Ltd. (Hangzhou, China). The mCherry-GFP-LC3b-adenovirus was purchased from Beyotime Institute of Biotechnology (Shanghai, China). The GAPDH antibody was obtained from Abcam (Cambridge, MA, USA). Light chain (LC) 3B, Beclin1, MMP-3, and MMP-13 antibodies were purchased from Novus Biologicals (Littleton, CO, USA).
4
Sodium Hydrosulfide Modulates NLRP3 Inflammasome
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Sodium hydrosulfide (NaHS) was purchased from Chuandong Chemical Group Co. Ltd. (Chongqing, China). N-Acetyl-L-cysteine (NAC) and 2,7-dichlorofluorescein diacetate (DCFH-DA) were manufactured by Sigma-Aldrich (St. Louis, MO, USA). Cell Counter Kit-8 (CCK-8) was purchased from Dojindo (Kumamoto, Japan). Dulbecco's modified Eagle's medium F12 (DMEM/F12) and fetal bovine serum (FBS) were purchased from Gibco-BRL (Carlsbad, CA). TRIzol RNA isolation reagent was purchased from Invitrogen Life Technologies (New York, New York, USA). The first strand cDNA synthesis kit and SYBR green reagents were purchased from Takara (Dalian, China). siRNA of NLRP3 was obtained from Hanbio Biotechnology Co. Ltd. (Shanghai, China).
5
NGR1 Neuroprotection and Oxidative Stress
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NGR1 (molecular weight = 933.15; purity > 98%) was purchased from Shanghai Winherb Medical S and T Development (Beijing, China). Dulbecco’s modified Eagle’s medium/F12 (DMEM/F12) and foetal bovine serum (FBS) were obtained from Gibco (Grand Island, NY, USA). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and fluorescent dye JC-1 were acquired from Enzo Life Sciences (New York, NY, USA). ELISA kits for 4-hydroxynonenal (4-HNE), 8-hydroxy-2′-deoxyguanosine (8-OHdG) and protein carbonyl were acquired from Expandbio (Beijing, China). An Annexin V/propidium iodide (PI) kit, MitoTracker® Red CM-H2XRos and a MitoSOX™ assay kit purchased from Invitrogen (Grand Island). A terminal deoxynucleotidyl transferase biotin-dUTP nick end labelling (TUNEL) detection kit was purchased from Roche Diagnostics GmbH, Mannheim, Germany. Cell protein extraction kits, PINK1 siRNA, and control siRNA were provided by Santa Cruz Biotechnology (Dallas, TX, USA). Bicinchoninic acid assay kits were purchased from Pierce Biotechnology (Waltham, MA, USA). The pCMV-G FP-LC3 expression vector was acquired from Cell Biolabs (San Diego, CA, USA).
6
Alendronate Effects on Cell Viability
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The following reagents were commercially obtained: Pro-Long Gold Antifade Reagent with DAPI, from Molecular Probes (Eugene, OR, USA); Dulbecco's modified Eagle's medium F12 (DMEM/F12) and fetal bovine serum (FBS), from Gibco (Gaithersburg, MD, USA); Alendronate, Dimethyl sulfoxide (DMSO), Hoechst 33342, RNase A, proteinase K, aprotinin, leupeptin, Triton X-100, PMSF and thiazolyl blue tetrazolium bromide (MTT), from Sigma (St. Louis, MO, USA); SuperSignal West Femto enhanced-chemiluminescence Western blotting detection reagent, from Pierce (Rockford, IL, USA).
7
Cytotoxicity Assessment of Mycotoxins
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T-2 toxin solution (NO. 34071, 100 μg/ml in acetonitrile), DON solution (NO. 34124, 100 μg/ml in acetonitrile) and 3-[4,5-Dimethylthiazol-2-yl]-2,5- diphenyltetrazolium bromide (MTT) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Dulbecco’s modified Eagle’s medium-F12 (DMEM-F12) and fetal bovine serum (FBS) were purchased from Gibco BRL (Grand Island, NY, USA). All other chemicals used were of analytical grade.
8
RSV Detection Protocol
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RSV 200808) was provided from Santa Cruz Biotechnology-USA. Dulbecco’s modified Eagle’s medium F12 (DMEM-F12) was obtained from Gibco-UK. Fetal bovine serum (FBS), Dimethyl sulfoxide (DMSO), penicillin, and streptomycin were purchased from Sigma-USA. RNeasy plus mini kit (74134) was provided from QIAGEN-UK. PrimeScriptTM RT reagent Kit (RR037A) and SYBR Green Real-Time PCR Master Mix (RR820L) were obtained from TaKaRa-Japan.
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RSV 200808) was provided from Santa Cruz Biotechnology-USA. Dulbecco’s modified Eagle’s medium F12 (DMEM-F12) was obtained from Gibco-UK. Fetal bovine serum (FBS), Dimethyl sulfoxide (DMSO), penicillin, and streptomycin were purchased from Sigma-USA. RNeasy plus mini kit (74134) was provided from QIAGEN-UK. PrimeScriptTM RT reagent Kit (RR037A) and SYBR Green Real-Time PCR Master Mix (RR820L) were obtained from TaKaRa-Japan.
9
Corneal Stromal Equivalent Construction from SMILE Lenticules
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Human corneal lenticules were extracted during SMILE procedures using the VisuMax Femtosecond Laser System (Carl Zeiss Meditec AG, Jena, Germany) as previously described17 (link). Only lenticules with a diameter of 6.6 mm and a central thickness of ≥100 μm were selected for the construction of corneal stromal equivalents. A 10-0 nylon suture was applied with the knot tied on the superior aspect of the lenticule to determine the correct side at the time of the subsequent procedure. The lenticule was immediately transferred to a sterile Eppendorf tube that was pre-filled with Dulbecco’s modified Eagle’s medium/F12 (DMEM/F12; Gibco, NY, USA) containing 100 U/ml penicillin G, 100 μg/ml streptomycin sulfate, and 1 μg/ml amphoptericin B (Invitrogen, Carlsbad, USA) and transported at 4 °C to the laboratory. The lenticule was rinsed 3 times with phosphate-buffered saline (PBS). For construction of the corneal stromal equivalent, 2 lenticules derived from the same donor were adhered to each other by fibrin glue (Shanghai Raas Blood Products Co., Ltd, Shanghai, China) with the superior aspect upward (Fig. 1 ).
10
Profiling Serum and Tissue Specimens from Hepatocellular Carcinoma Patients
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Human serum specimens were collected from healthy donors and HCC patients before resection in the Eastern Hepatobiliary Surgery Hospital in Shanghai, China. A tissue microarray composed of HCC samples from 85 patients was also obtained from the Eastern Hepatobiliary Surgery Hospital. The clinicopathological features of 85 patients are displayed in Supplementary Table 1 . The lung metastasis tissues from HCC were obtained from HCC patients in Shanghai Pulmonary Hospital. The human primary CAFs or NFs were isolated from fresh tumor tissues of HCC or normal liver tissues with the tumor dissociation kit (Miltenyi Biotec) and cultured in Dulbecco’s modified Eagle’s medium/F12 (DMEM/F12) (Gibco, Carlsbad, CA, USA), supplemented with 10% fetal bovine serum (FBS) (Gibco). All procedures were conducted with the approval of the Ethical Committee of the Second Military Medical University and Shanghai Pulmonary Hospital. Patient consent was obtained before the start of the study.
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