4 dimethylaminopyridine dmap

Docetaxel injection (Duopafei^®) was purchased from Qi Lu Pharmaceutical Co., Ltd. (Jinan, China). Acetonitrile and methanol were supplied by Fisher (USA, chromatographic grade). Docetaxel was provided by Shanghai Boyle chemical Co., Ltd (Shanghai, China). Bletilla striata polysaccharides were purchased from Shanxi Pioneer Biotech Co., Ltd (Shanxi, China). 4-Dimethylaminopyridine (DMAP) and 1-ethyl-3-[3-(dimethyl amino) propyl] carbodiimide (EDC) were supplied by Energy Chemical Co., Ltd. (Shanghai, China). Stearic acid (SA) was purchased from Sino pharm Chemical Regent Co., Ltd (Beijing, China). All the other reagents used were of analytical purity grade and obtained commercially.

2,2′-Azobis(2-methylpropionitrile) (AIBN; Aladdin) was recrystallized from 95% ethanol. Buf (99%) was purchased from Chengdu Aifa Biotech Co., Ltd. (Chengdu, China) and used as received. Et₃N, 2-Bromoethanol, 4-dimethylaminopyridine (DMAP), CuSO₄·5H₂O, Vitamine C sodium salt and Pent-4-ynoic acid were purchased from Energy Chemical Co., Ltd. (Shanghai, China) and used as received. D-(+)-Mannose was purchased from Aladdin Co., Ltd. (Shanghai, China) and used as received. KN₃ and acryloyl chloride were purchased from Macklin Biochemical Co., Ltd. (Shanghai, China) and used as received. N,N′-dicyclohexylcarbodiimide (DCC) and 2,2′-azobis [2-(2-imidazolin-2-yl)propane]dihydrochloride (VA-044) were purchased from Bidepharm Co., Ltd. (Shanghai, China) and used as received. Propargylamine was purchased from Tokyo Chemical Industry Co., Ltd. (Shanghai, China) and used as received. LysoTracker^®Red DND-99, Fetal bovine serum (FBS), penicillin, streptomycin, and Dulbecco’s Modified Eagle’s Medium (DMEM) were purchased from Thermo Fisher Scientific (Waltham, MA, USA) and used as received. Hoechst 33342 was purchased from Maokang Biotechnology Co., Ltd. (Shanghai, China) and used as received. The human liver cancer cell line HepG2 was purchased from the Cell Bank of Type Culture Collection of Chinese Academy of Sciences (Shanghai, China).

Dextran (Mn = 70,000 g mol⁻¹) was purchased from Aladdin. GO suspension (20 mg/mL) was obtained from XF NANO Materials Tech Co. (Nanjing China). 4-Formylbenzoic acid and cyanoacetic acid were bought from Alfa Aesar. Histidine was bought from Aladdin. 4-Dimethylaminopyridine (DMAP) was bought from Energy Chemical. 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC·HCl) was bought from GL Biochem Ltd. Dimethyl sulfoxide (DMSO) was obtained from Aladdin. Cell Counting Kit-8, calcein-AM, and propidium iodide were obtained from Sigma-Aldrich. Bovine albumin (BSA) and cell culture media were bought from Gibco. NIH 3T3 cells were purchased from the American Type Culture Collection (Rockville, MD) and were grown in Dulbecco's modified Eagle's medium (DMEM).