Tracefinder 2

Manufactured by Thermo Fisher Scientific

Tracefinder 2.1 is a software platform developed by Thermo Fisher Scientific for the analysis and identification of trace-level compounds in complex samples. The software provides advanced data processing and reporting capabilities to support analytical workflows.

Automatically generated - may contain errors

Lab products found in correlation

Xcalibur 4, by Thermo Fisher Scientific (2 mentions) Luna nh2 column, by Phenomenex (1 mentions) Tsq quantiva triple quadrupole ms, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

TraceFinder (72 citations)

3 protocols using tracefinder 2

Metabolite Profiling of Cell Culture Media

Check if the same lab product or an alternative is used in the 5 most similar protocols

10 μL of extracted cultured media were separated using a
150 × 2.0 mm Luna NH2 column (Phenomenex); mobile phase A: 20mM
ammonium acetate, 20mM ammonium hydroxide in water; mobile phase B: l0mM
ammonium hydroxide in 10% water/22.5% methanol/67.5% acetonitrile. The
column was eluted isocratically with a linear gradient from 100% to 0%
mobile phase B over 10 minutes, then 2 minutes at 0% mobile phase B. MS
data were acquired with multiple reaction monitoring in the negative ion
mode on a TSQ Quantiva triple quadrupole MS (Thermo Fisher Scientific).
Raw data were integrated and visually inspected using TraceFinder 2.1
software (Thermo Fisher Scientific). MRM transitions were as follows:
pyruvate (87/43), lactate (89.1/43.1), lactate-d3 (92.1/45.1).
Metabolite identifications were confirmed using synthetic mixtures of
reference compounds.

Kim W., Deik A., Gonzalez C., Gonzalez M.E., Fu F., Ferrari M., Churchhouse C.L., Florez J.C., Jacobs S.B., Clish C.B, & Rhee E.P. (2019). Polyunsaturated Fatty Acid Desaturation Is a Mechanism for Glycolytic NAD+ Recycling. Cell metabolism, 29(4), 856-870.e7.

+ Open protocol

+ Expand

Methanol Extraction and HPLC-MS/MS Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were collected and pellets were flash frozen in liquid nitrogen. Frozen pellets were stored at −80°C until extraction. Samples were thawed and resuspended in 1 mL of ice cold 80% methanol (HPLC grade) and subjected to three freeze–thaw cycles alternating between liquid nitrogen and a 37°C water bath. Next, samples were centrifuged at 20,000 g for 15 minutes at 4°C. The resulting supernatants were transferred to fresh tubes and dried. Samples were resuspended in 10 μL per 200,000 cells. Samples were analyzed by high-performance liquid chromatography and high-resolution mass spectrometry and tandem mass spectrometry (HPLC–MS/MS). The sample volumes of 10 μL, which contained 200,000 cells, were injected. Data acquisition and analysis were carried out by Xcalibur 4.0 software and Tracefinder 2.1 software, respectively (both from ThermoFisher, MA).

Gopal P., Yard B.D., Petty A., Lal J.C., Bera T.K., Hoang T.Q., Buhimschi A.D, & Abazeed M.E. (2022). The Mutational Landscape of Cancer's Vulnerability to Ionizing Radiation. Clinical Cancer Research, 28(24), 5343-5358.

+ Open protocol

+ Expand

Metabolomic Analysis of Frozen Cell Pellets

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were collected and pellets were flash frozen in liquid nitrogen. Frozen pellets were stored at −80 °C until extraction. Samples were thawed and resuspended in 1 mL of ice cold 80% methanol (HPLC grade) and subjected to three freeze–thaw cycles alternating between liquid nitrogen and a 37 °C water bath. Next, samples were centrifuged at 20,000g for 15 min at 4 °C. The resulting supernatants were transferred to fresh tubes and dried. Samples were resuspended in 10 μl per 200,000 cells. Samples were analyzed by high-performance liquid chromatography and high-resolution mass spectrometry and tandem mass spectrometry (HPLC–MS/MS). The sample volumes of 10 μl, which contained 200,000 cells, were injected. Data acquisition and analysis were carried out by Xcalibur 4.0 software and Tracefinder 2.1 software, respectively (both from ThermoFisher, MA).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!