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Diff quick staining solution

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Diff-Quick staining solution is a three-step differential staining method used for the rapid identification of cells and their morphological characteristics in various clinical and research applications. The solution consists of a fixative, a first stain, and a second stain, which together provide a clear and consistent staining pattern for microscopic examination.

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Lab products found in correlation

Type 1 collagen, by BD (2 mentions) 24 well boyden chambers with 8 μm pore polycarbonate membranes, by Corning (2 mentions) Ix81 inverted microscope, by Olympus (2 mentions) 8μm pore polycarbonate membranes, by Corning (2 mentions) Matrigel, by BD (2 mentions) Ix81 microscope, by Olympus (2 mentions)

Close spelling variants of this product

Diff-Quick (84 citations) Diff-Quick staining (9 citations) Diff-Quick solution (6 citations)

6 protocols using diff quick staining solution

1

Transwell Migration Assay for NDL Cells

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Primary NDL cells were subjected to the transwell migration assay as described49 , with some modifications. Cells were plated directly onto the transwell in serum starve media (0.1% FBS) at a density of 2.5 × 104 cells/mL and migration was permitted for 16hrs. Migrated cells were fixed and stained with Diff-Quick staining solution (Dade Behring, Deerfield, IL, USA) prior to imaging with an Olympus IX81.
Rowson-Hodel A.R., Wald J.H., Hatakeyama J., O’Neal W.K., Stonebraker J.R., VanderVorst K., Saldana M.J., Borowsky A.D., Sweeney C, & Carraway KL I.I.I. (2017). Membrane Mucin Muc4 Promotes Blood Cell Association with Tumor Cells and Mediates Efficient Metastasis in a Mouse Model of Breast Cancer. Oncogene, 37(2), 197-207.
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2

Quantifying Cell Migration and Invasion

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Cells transduced with LRIG1 or control pMX vector were plated at 40,000 cells/well in 24-well Boyden chambers with 8 μm-pore polycarbonate membranes (Corning, Lowell, MA, USA) according to the manufacturer’s protocol. Cells were seeded in serum-free media and allowed to migrate for 12 hrs toward the lower chamber containing media with 10% FBS. In some experiments, the c-Met-specific inhibitor 0.5 μM ARQ197 (Active Biochemicals, Selleck, USA) was added in the upper and low compartments. After 12 hrs, the cells were fixed and stained with Diff-Quick staining solution (Dade Behring, Newark, DE, USA). For the cell invasion assay, the chambers were pre-coated with collagen type I (BD Bioscience San Diego, CA). Migrated cells were imaged and counted in 10 microscopic fields on an Olympus IX81 inverted microscope with cellSens Entry software using a 10x objective. The results were averaged among three independent experiments.
Yokdang N., Hatakeyama J., Wald J.H., Simion C., Tellez J.D., Chang D.Z., Swamynathan M.M., Chen M., Murphy W.J., Carraway KL I.I.I, & Sweeney C. (2015). LRIG1 opposes epithelial to mesenchymal transition and inhibits invasion of basal-like breast cancer cells. Oncogene, 35(22), 2932-2947.
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3

Quantifying Cell Migration and Invasion

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Cells transduced with LRIG1 or control pMX vector were plated at 40,000 cells/well in 24-well Boyden chambers with 8 μm-pore polycarbonate membranes (Corning, Lowell, MA, USA) according to the manufacturer’s protocol. Cells were seeded in serum-free media and allowed to migrate for 12 hrs toward the lower chamber containing media with 10% FBS. In some experiments, the c-Met-specific inhibitor 0.5 μM ARQ197 (Active Biochemicals, Selleck, USA) was added in the upper and low compartments. After 12 hrs, the cells were fixed and stained with Diff-Quick staining solution (Dade Behring, Newark, DE, USA). For the cell invasion assay, the chambers were pre-coated with collagen type I (BD Bioscience San Diego, CA). Migrated cells were imaged and counted in 10 microscopic fields on an Olympus IX81 inverted microscope with cellSens Entry software using a 10x objective. The results were averaged among three independent experiments.
Yokdang N., Hatakeyama J., Wald J.H., Simion C., Tellez J.D., Chang D.Z., Swamynathan M.M., Chen M., Murphy W.J., Carraway KL I.I.I, & Sweeney C. (2015). LRIG1 opposes epithelial to mesenchymal transition and inhibits invasion of basal-like breast cancer cells. Oncogene, 35(22), 2932-2947.
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4

Cell Migration and Invasion Assay

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Cells were plated on chambers with 8μm-pore polycarbonate membranes (Corning) and seeded in DMEM with 0.01% FCS, migrating overnight toward the lower chamber containing DMEM with 10% FCS. Migrated cells were fixed and stained with Diff-Quick staining solution (Dade Behring). For cell invasion assays, chambers were pre-coated with Matrigel (BD Bioscience). Cells that migrated or invaded were imaged and counted in five microscopic fields per filter on an Olympus IX81 microscope with cellSens Entry software. Results were normalized to proliferation rates and averaged among at least three independent experiments.
Wald J.H., Hatakeyama J., Printsev I., Cuevas A., Fry W.H., Saldana M.J., Vorst K.V., Rowson-Hodel A., Angelastro J.M., Sweeney C, & Carraway KL I.I.I. (2017). Suppression of planar cell polarity signaling and migration in glioblastoma by Nrdp1-mediated Dvl polyubiquitination. Oncogene, 36(36), 5158-5167.
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5

Cell Migration and Invasion Assay

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Cells were plated on chambers with 8μm-pore polycarbonate membranes (Corning) and seeded in DMEM with 0.01% FCS, migrating overnight toward the lower chamber containing DMEM with 10% FCS. Migrated cells were fixed and stained with Diff-Quick staining solution (Dade Behring). For cell invasion assays, chambers were pre-coated with Matrigel (BD Bioscience). Cells that migrated or invaded were imaged and counted in five microscopic fields per filter on an Olympus IX81 microscope with cellSens Entry software. Results were normalized to proliferation rates and averaged among at least three independent experiments.
Wald J.H., Hatakeyama J., Printsev I., Cuevas A., Fry W.H., Saldana M.J., Vorst K.V., Rowson-Hodel A., Angelastro J.M., Sweeney C, & Carraway KL I.I.I. (2017). Suppression of planar cell polarity signaling and migration in glioblastoma by Nrdp1-mediated Dvl polyubiquitination. Oncogene, 36(36), 5158-5167.
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6

Transwell Migration Assay for NDL Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Primary NDL cells were subjected to the transwell migration assay as described49 , with some modifications. Cells were plated directly onto the transwell in serum starve media (0.1% FBS) at a density of 2.5 × 104 cells/mL and migration was permitted for 16hrs. Migrated cells were fixed and stained with Diff-Quick staining solution (Dade Behring, Deerfield, IL, USA) prior to imaging with an Olympus IX81.
Rowson-Hodel A.R., Wald J.H., Hatakeyama J., O’Neal W.K., Stonebraker J.R., VanderVorst K., Saldana M.J., Borowsky A.D., Sweeney C, & Carraway KL I.I.I. (2017). Membrane Mucin Muc4 Promotes Blood Cell Association with Tumor Cells and Mediates Efficient Metastasis in a Mouse Model of Breast Cancer. Oncogene, 37(2), 197-207.
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