Dl dithiothreitol

The urea was purchased from Amresco (Solon, OH, USA). Thiourea was purchased from Pharmabiology. DL-dithiothreitol (DTT), ethylene diamine tetraacetic acid (EDTA), sodium dodecyl sulfate (SDS), and Tris base were purchased from Solarbio (Shanghai, China). Phenylmethanesulfonyl fluoride (PMSF) and protein marker was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Iodoacetamide (IAM) was purchased from Aladdin (Shanghai, China). HPLC-grade acetone, acetonitrile, and formic acid were purchased from TEDIA Company Inc (Fairfield, OH, USA). Cellulose, pectolyase, and macerozyme were purchased from Sigma-Aldrich (St. Louis, MO, USA). Mannitol and other common chemicals and reagents were obtained from the Shanghai Sangon Biotech Company (Shanghai, China). Protein Assay Dye Reagent Concentrate was purchased from Bio-Rad (Hercules, CA, USA). An SDS-PAGE gel preparation kit was purchased from Beyotime (Shanghai, China). Ultrafilter (10 kD) was purchased from Merck-Millipore (Darmstadt, Germany). Milli-Q water was used in all experiments.

Albumin from bovine serum was procured from Wuhan Chucheng Zhengmao Science and Technology Engineering Co., Ltd. DL-Dithiothreitol were procured from Solarbio, while EDTA, Xylene brilliant cyaninG-250, Sodium dodecyl sulfate, ThioUrea, and Acetone were procured from Sinopharm. Iodoacetamide was procured from Aladdin, and Phenylmethanesulfonyl fluoride was procured from Xiya Reagent. Tetraethylammonium bromide and Urea were procured from Sigma. Trypsin was procured from Promega, and the protein marker was procured from Fementas. Lastly, the bicinchoninic acid (BCA) protein quantification kit was procured from Biyuntian. ELISA kits for myosin heavy chain 9 (MYH9) and fetuin B (FETUB) were procured from Wuhan Fearn and Eliot Biotechnology Co.

CA (C110084) was purchased from Aladdin (Shanghai, China). 3-Phenylpropanal (BD17390) was purchased from Bidepharm (Shanghai, China). Cinnamic acid (C804991) was obtained from Macklin (Shanghai, China). FTM (S87283) and TAX (B21695) were purchased from Yuanye (Shanghai, China). DITC (F21540) was purchased from MERYER (Shanghai, China). Pt (HY-17394) was purchased from MedChemExpress (MCE, Monmouth, NJ, USA). TMZ (AK-72945) was purchased from Ark Pharm (Chicago, IL, USA). D,L-dithiothreitol was purchased from Solarbio (DTT, A285, Beijing, China). AP-III-a4 (HY-15858A) was purchased from MCE. Antibodies against Bax (50599-2-lg), Bcl₂ (60178-1-lg), and Bid (10988-1-AP) were obtained from Proteintech (Chicago, IL, USA); ENO1 (bs-3978R), anti-GADPH (bsm-0978M), and β-actin (bs-0061R) antibodies were purchased from Bioss (Beijing, China). Alexa-Fluor-594-conjugated goat anti-rabbit IgG (ab150084) was purchased from Abcam (Cambridge, MA, UK), and a goat anti-rabbit IgG (#7074) secondary antibody was obtained from CST (Beverly, MA, USA).