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Prdm1

Manufactured by Thermo Fisher Scientific
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PRDM1 is a laboratory equipment product offered by Thermo Fisher Scientific. It is a specialized instrument designed for performing specific laboratory tasks. The core function of PRDM1 is to [core function description]. No further details or interpretation on the intended use of this product can be provided in a concise and unbiased manner.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions) Gapdh, by Thermo Fisher Scientific (2 mentions) Nanos3, by Thermo Fisher Scientific (2 mentions) Tfap2c, by Thermo Fisher Scientific (2 mentions) Pou5f1, by Thermo Fisher Scientific (2 mentions) Prdm14, by Thermo Fisher Scientific (2 mentions) Taqman universal pcr master mix, by Thermo Fisher Scientific (2 mentions) Superscript 2 reverse transcriptase, by Thermo Fisher Scientific (2 mentions) Rlt buffer, by Qiagen (2 mentions) Rneasy micro kit, by Qiagen (2 mentions) Superscript 2, by Thermo Fisher Scientific (1 mentions) 7500 real time pcr system, by Thermo Fisher Scientific (1 mentions) 18s rrna, by Thermo Fisher Scientific (1 mentions) Mmp 9, by Thermo Fisher Scientific (1 mentions) C fos, by Thermo Fisher Scientific (1 mentions) Taqman universal master mix 2, by Thermo Fisher Scientific (1 mentions) Fast red violet lb salt, by Merck Group (1 mentions) Hplc grade solvents, by Thermo Fisher Scientific (1 mentions) Formic acid, by Samchun (1 mentions)

Close spelling variants of this product

TaqMan Gene Expression assays for PRDM1 Prdm1; Mm00476128_m1

4 protocols using prdm1

1

Profiling Pluripotency Genes in Germ Cell-Like Cells

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PGCLCs sorted or harvested from membranes were placed in 350 μL of RLT buffer (QIAGEN) and RNA was extracted using RNeasy micro kit (QIAGEN, 74004). cDNA was synthesized using SuperScript® II Reverse Transcriptase (Invitrogen, 18064-014). Real time quantitative PCR was performed using TaqMan® Universal PCR Master Mix (Applied Biosystems, 4304437) and the expression level of genes-of-interest were normalized to the expression of housekeeping gene GAPDH. The TaqMan probes used in this study include: GAPDH (Applied Biosystems, Hs99999905_m1), NANOS3 (Applied Biosystems, Hs00928455_s1), PRDM1 (Applied Biosystems, hs01068508_m1), TFAP2C (Applied Biosystems, Hs00231476_m1), POU5F1 (Applied Biosystems, Hs03005111_g1), SOX2 (Applied Biosystems, Hs01053049_s1), PRDM14 (Applied Biosystems, Hs01119056_m1).
Gell J.J., Zhao J., Chen D., Hunt T.J, & Clark A.T. (2018). PRDM14 is expressed in germ cell tumors with constitutive overexpression altering human germline differentiation and proliferation. Stem cell research, 27, 46-56.
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2

Profiling Pluripotency Genes in Germ Cell-Like Cells

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PGCLCs sorted or harvested from membranes were placed in 350 μL of RLT buffer (QIAGEN) and RNA was extracted using RNeasy micro kit (QIAGEN, 74004). cDNA was synthesized using SuperScript® II Reverse Transcriptase (Invitrogen, 18064-014). Real time quantitative PCR was performed using TaqMan® Universal PCR Master Mix (Applied Biosystems, 4304437) and the expression level of genes-of-interest were normalized to the expression of housekeeping gene GAPDH. The TaqMan probes used in this study include: GAPDH (Applied Biosystems, Hs99999905_m1), NANOS3 (Applied Biosystems, Hs00928455_s1), PRDM1 (Applied Biosystems, hs01068508_m1), TFAP2C (Applied Biosystems, Hs00231476_m1), POU5F1 (Applied Biosystems, Hs03005111_g1), SOX2 (Applied Biosystems, Hs01053049_s1), PRDM14 (Applied Biosystems, Hs01119056_m1).
Gell J.J., Zhao J., Chen D., Hunt T.J, & Clark A.T. (2018). PRDM14 is expressed in germ cell tumors with constitutive overexpression altering human germline differentiation and proliferation. Stem cell research, 27, 46-56.
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3

Gene Expression Profiling of Immune Cells

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Total RNA was isolated and cDNA was reversely transcribed using Superscript II (Gibco BRL) according to manufacturer's instructions. Real‐time quantitative polymerase chain reaction was performed for selected genes. We tested genes TBX21 (Hs00203436_m1), GATA3 (Hs00231122_m1), RORC (Hs01076122_m1), FOXP3 (Hs01085834_m1), HSP60 (Hs01102298_g1), HSP90 (Hs00743767_sH), (Hs00356629_g1), and PRDM1 (Hs00153357_m1; Applied Biosystems, CA). The expression level of the housekeeping gene GAPDH (Hs02758991_g1) was used to normalize for differences in input cDNA. Real‐time quantitative polymerase chain reaction was carried out using TaqMan gene expression presynthesized reagents and master mix (Applied Biosystems, CA) in Applied Biosystems 7500 Real‐Time PCR System. The expression ratio was calculated using the ΔΔCT method.
Frostegård J., Zhang Y., Sun J., Yan K, & Liu A. (2016). Oxidized Low‐Density Lipoprotein (OxLDL)–Treated Dendritic Cells Promote Activation of T Cells in Human Atherosclerotic Plaque and Blood, Which Is Repressed by Statins: microRNA let‐7c Is Integral to the Effect. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 5(9), e003976.
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4

Osteoclast Differentiation Assay

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Formic acid was acquired from Samchun (Pyeongtaek, Republic of Korea). All HPLC-grade solvents and fetal bovine serum (FBS) were obtained from Thermo Fisher Scientific (Waltham, MA, USA). Minimum Essential Medium, Alpha Modification (α-MEM), was purchased from Hyclone (Logan, UT, USA). AS-MX phosphate, fast red violet LB salt, and naphthol were procured from Sigma-Aldrich (St. Louis, MO, USA). Antibodies targeting c-Fos and NFATc1 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies targeting TRAF6, c-Src, CAII and HRP-conjugated secondary antibodies (anti-rabbit IgG and anti-mouse IgG) were purchased from Cell Signaling Technology (Danvers, MA, USA). TaqMan Universal Master Mix II and TaqMan probes for the target genes, including c-Fos, NFATc1, Prdm1, Irf8, MafB, Ctsk, Itgβ3, Mmp9, and 18S rRNA, were obtained from Applied Biosystems (Foster City, CA, USA). TSN standard was purchased from ChemFace (Wuhan, China).
Kim S.C., Gu D.R., Yang H., Lee S.J., Ryuk J.A, & Ha H. (2023). Isolation and Characterization of an Anti-Osteoporotic Compound from Melia toosendan Fructus. Pharmaceutics, 15(10), 2454.
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