Tissue sections were immunostained as previously described [28 (link)]. Primary antibodies included rabbit anti-Ki67 (Thermo Fisher Scientific, Inc., Fremont, CA; Cat. No. RM-9106-S1) (1:200), mouse anti-BrdU (Thermo; Cat. No. MS-1058-PO) (1:250), goat anti-cryptdin related sequence 4C (CRS4C) (gift from Dr. A. J. Ouellette, University of Southern California, Los Angeles, CA) [32 (link)] (1:2000), and rabbit anti-MUC2 (Santa Cruz Biotechnology, Inc, Santa Cruz, CA; Cat. No. sc15334) (1:100). Secondary antibodies included biotinylated donkey anti-rabbit IgG, donkey anti-goat IgG, and donkey anti-mouse IgG (all from Vector Labs, Burlingame, CA). Biotinylated antibodies were linked to avidin-horseradish peroxidase conjugates (Vector Labs), visualized using 3,3′-diamino benzidine (Sigma) for 2 to 5 min, and lightly counterstained with hematoxylin.
Avidin horseradish peroxidase conjugates
Manufactured by Vector Laboratories
Sourced in United States
Avidin-horseradish peroxidase conjugates are a type of lab equipment used in various biochemical applications. They consist of the protein avidin, which has a high affinity for the vitamin biotin, coupled with the enzyme horseradish peroxidase. This conjugate can be used to detect and quantify the presence of biotinylated molecules in samples.
Automatically generated - may contain errors
Lab products found in correlation
Biotinylated donkey anti rabbit igg, by Vector Laboratories (4 mentions)
3 3 diaminobenzidine (dab), by Merck Group (4 mentions)
Rabbit anti ki67, by Thermo Fisher Scientific (3 mentions)
Mouse anti brdu, by Thermo Fisher Scientific (1 mentions)
Rabbit anti muc2, by Santa Cruz Biotechnology (1 mentions)
Donkey anti mouse igg, by Vector Laboratories (1 mentions)
Anti ki67, by Thermo Fisher Scientific (1 mentions)
4 protocols using avidin horseradish peroxidase conjugates
1
Immunohistochemical Analysis of Intestinal Markers
2
Intestinal cell proliferation analysis
3
Intestinal cell proliferation analysis
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The ileal tissues were immunostained using a rabbit anti-Ki67 (Thermo Fisher Scientific, Waltham, MA, Cat. No. RM- 9106-S1) antibody followed by a biotinylated donkey anti-rabbit IgG, (Vector Labs, Burlingame, CA) and then by avidin-horseradish peroxidase conjugates (Vector Labs). Positive cells were visualized after incubation with 3,3′-diaminobenzidine (Sigma-Aldrich) for 2 to 5 min and counterstained with hematoxylin. The total number of Ki67 positive cells was determined for each villous-crypt axis. Only well-oriented crypts with the epithelial layer on at least one side continuous with the villous epithelial layer were counted.
4
Immunohistochemical Analysis of Intestinal Cell Proliferation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Ileal tissues were immunostained using a rabbit anti-Ki67 (Thermo Fisher Scientific, Waltham, MA, USA; RM-9106-S1) antibody followed by a biotinylated donkey anti-rabbit IgG (Vector Laboratories, Burlingame, CA, USA) and then by avidin-horseradish peroxidase conjugates (Vector Laboratories). Positive cells were visualized after incubation with 3,3′-diaminobenzidine (Sigma-Aldrich) for 2-5 min and counterstained with Hematoxylin. The total number of Ki67-positive cells was determined for 100 crypts each slide.
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