Survival endpoint criteria were defined as meeting one of the following conditions: unable to return to the normal, upright position within 10 s following push over, or paralysis of hind limbs. Animals with survival time 2 or more standard deviations outside the mean were excluded from the survival curve. When the mice reached the endpoint, body weight was recorded and mice were humanely euthanized by IP injection of SomnaSol (50 mg/kg, Butler Schein, NDC #11695-4829-1.) Blood was collected by tail snip, and blood glucose and βHB were measured with the Precision Xtra Blood Glucose & Ketone Monitoring System (Abbott Inc.). Organs and tissue samples (Table 4 ) were harvested immediately, and their weights were recorded.
Precision xtra blood glucose ketone monitoring system
Manufactured by Abbott
Sourced in United States
The Precision Xtra Blood Glucose & Ketone Monitoring System is a compact, portable device designed to measure blood glucose and ketone levels. It provides accurate and reliable results for monitoring these important health indicators.
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Lab products found in correlation
7 protocols using precision xtra blood glucose ketone monitoring system
1
Survival Endpoint Criteria and Tissue Analysis
2
Monitoring Metabolic Parameters in VM-M3 Mice
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Blood was collected once weekly from the tail of the VM-M3 cancer mice using approved methods. Blood glucose was measured with the Nova Max® Plus Glucose and β-Ketone Monitoring System (Nova Biomedical, Waltham, MA), and blood βHB was measured with the Precision Xtra™ Blood Glucose & Ketone Monitoring System (Abbott Laboratories). Mice were weighed between 1 and 3 pm, twice weekly, for the duration of the study using the AWS-1KG Portable Digital Scale (AWS, Charleston, SC).
3
Glucose and Ketone Monitoring in Fasted State
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Blood glucose and ketone readings were assessed after 15-h overnight water-only fast (baseline) and hourly after consuming study foods for 4 h using the Precision Xtra Blood Glucose & Ketone Monitoring System (Abbott), a widely used point-of-care kit which had been validated against standard laboratory method in animals and humans [39 (link),40 (link),41 (link),42 (link),43 (link),44 (link)].
4
Metabolite Quantification in Mice
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Blood glucose and ketone (R β‐hydroxybutyrate) concentration were measured using Precision Xtra™ Blood Glucose & Ketone Monitoring System (Abbott Laboratories, Abbott Park, IL, USA). Blood lactate concentration was measured using Lactate Plus Lactate Meter (Nova Biomedical). IGF‐1 and insulin were relabeled for blinded analysis and subsequently analyzed using Luminex 100 system (Luminex, Austin, TX, USA) with R&D Systems Mouse 1‐Plex Luminex Assay (R&D Systems, Minneapolis, MN, USA) and Milliplex Mouse Multiplex Kit (Millipore, St. Charles, MO, USA), respectively, using manufacturer's protocol.
5
Dietary Adherence Measurement and Ketosis Monitoring
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An average of available dietary recalls and records at each time point were used to calculate adherence scores at 6 time points from NDS-R diet data to measure degrees of adherence to the dietary recommendations. A description of score components is provided in Supplemental Tables 1 2
6
Nutritional Ketosis Monitoring in Clinical Study
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While participants were completing the WFKD phase of the study, they were provided with blood ketone monitors and strips (Abbott Precision Xtra Blood Glucose & Ketone Monitoring System) to measure ketones three times/week before breakfast (fasting state). Health educators reviewed these values with participants and used these to adjust carbohydrate intake recommendations to try to achieve a ketone range of 0.5–3.0 mmol/L. Ketone levels of 0.5 to 1.5 mmol/L indicate light nutritional ketosis and levels from 1.5 to 3.0 mmol/L indicate optimal nutritional ketosis [22 ]. Additionally, participants completed a fasting venous blood draw at seven time points throughout the study, prebaseline, baseline, 4 weeks, 12 weeks (end of diet 1), 16 weeks, 24 weeks (end of diet 2), and 36 weeks (follow-up). β-hydroxybutyrate was analyzed by the Core Laboratory for Clinical Studies Washington University School of Medicine (St. Louis, MO, USA) on EDTA plasma samples. Levels were measured by an enzymatic colorimetric method using β-Hydroxybutyrate Liquicolor reagents from Stanbio Laboratory (distributed by Sekisui Diagnostics) and were ran on a Roche cobas c501 analyzer.
7
Weekly Blood Glucose and Ketone Monitoring
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Blood (approximately 10 μl) was collected once weekly from the tail using IACUC-approved methods. Blood glucose and β-hydroxybutyrate (βHB) was measured using the Precision Xtra Blood Glucose & Ketone Monitoring System (Abbott Laboratories). Mice were weighed in the mid-afternoon, twice weekly for the duration of the study using the AWS-1KG Portable Digital Scale (AWS).
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