Chemical inhibitors were obtained from the following suppliers and used at the indicated concentrations: KU55933 (ATMi) (Hickson et al., 2004 (link)) (118500, Millipore, 20 μM), VE-821 (ATRi) (Reaper et al., 2011 (link)) (A11605, Adooq Bioscience, 20 μM), SB218078 (Chk1i) (559402, Millipore, 5 μM), AZD7762 (Chk1/2i) (SML-0350, Sigma, 2 μM), MG-132 (Sigma, 25 μM), CHX (C7698, Sigma, 50 μM), AraC (C1768, Sigma, 50/5 μM), HAMNO (SML1234, Sigma, 20 μM), KU60019 (HY-12061, Insight Biotechnology, 10 μM), VE-822 (10 μM), AZ20 (Foote et al., 2013 (link)) (HY-15557, Insight Biotechnology, 15 μM), T2AA (SML0794, Sigma, 20 μM), KPT-251 (5005050001, Millipore, 20 μM), bortezomib (2 μM), carfilzomib (17554, Cambridge Bioscience, 2 μM), and CldU (C6891, Sigma, 5 μM).
Ve 821
Manufactured by Adooq
Sourced in United States
VE-821 is a lab equipment product. It serves a core function, but a detailed unbiased description cannot be provided without extrapolation.
Automatically generated - may contain errors
Lab products found in correlation
Ku55933, by Merck Group (2 mentions)
Cycloheximide (chx), by Merck Group (1 mentions)
Azd7762, by Merck Group (1 mentions)
Hamno, by Merck Group (1 mentions)
Ara c, by Merck Group (1 mentions)
Mg132, by Merck Group (1 mentions)
Bortezomib, by Cambridge Bioscience (1 mentions)
Ku55933, by Selleck Chemicals (1 mentions)
Pd0325901, by Cayman Chemical (1 mentions)
Sb203580, by Merck Group (1 mentions)
Nu7441, by Selleck Chemicals (1 mentions)
Sp600125, by Selleck Chemicals (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Embryomax nucleosides, by Merck Group (1 mentions)
3 protocols using ve 821
1
Detailed Inhibitor Concentrations for DNA Damage Response
2
Myocyte Differentiation and Oxidative Stress
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Hydrogen peroxide mixed with a stabilizer (H2O2, Sigma) was added at various concentrations by diluting a 1 mM stock immediately before use on day 8 of myocyte differentiation after the depletion of 2ME for 1 day. N-acetylcysteine (NAC) was dissolved in water, and the pH was adjusted to 7.0. NAC was added to the cells 1 h before H2O2 stimulation. KU-55933 (Selleck Chemicals, USA), VE-821 (AdooQ BioScience, USA), NU-7441 (Selleck Chemicals), PD0325901 (Cayman Chemical, USA), SP600125 (Selleck Chemicals) and SB203580 (Sigma) were dissolved in DMSO (Sigma) and added 1 h before H2O2 stimulation. MMC was added at various concentration for 2 or 2 and a half h and then washed once and replaced with medium.
3
Cell Cycle Arrest and DNA Damage Response
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Where indicated, cells were treated with 2 mM hydroxyurea, 2.5 μm aphidicolin, 10 μm etoposide, 10 μm 5-fluorouracil, 1 μM cisplatin, 1 μM gemcitabine, 0.001 % methyl methanesulfonate, 100 nM doxorubicin, 3 μM camptothecin, 300 μM nucleosides, 10 nM afatinib, 30 nM lapatinib, 150 nM rapamycin, 15 μm MEK inhibitor U0126, 150 nM AKT inhibitor MK2206, 7.5 μm PI3K inhibitor LY294002, 1 μm ATR kinase inhibitor VE821 (AdooQ), CCT244747 (a kind gift from Prof. Ian Collins, ICR, London), 10 μm ATM kinase inhibitor KU55933 (Merck, Millipore), 100 nM UCN01 Chk1/PKCβ inhibitor (Merck, Millipore), 12.5–300 μM EmbryoMax Nucleosides (Millipore).
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