Mice were subcutaneously injected with 1.5 µg of filgrastim or the same volume of PBS, shortly after the injection of C. perfringens. To inhibit G-CSFR, a neutralizing antibody against G-CSFR (R&D systems, Minneapolis, MN, USA) was used as described in a previous report [33 (link)]. The neutralizing antibody or Rat IgG2B isotype control (R&D systems, Minneapolis, MN, USA) was intraperitoneally administered to C. perfringens-infected mice at 5 µg per mouse shortly after the injection of C. perfringens. This dose was 10 times higher than that in the previous report [33 (link)].
Rat igg2b isotype control
Manufactured by R&D Systems
Sourced in United States, United Kingdom
The Rat IgG2B Isotype Control is a laboratory reagent used to establish the background level of immunostaining in immunological assays involving rat samples. It serves as a control to differentiate specific antibody binding from non-specific binding.
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Lab products found in correlation
2 protocols using rat igg2b isotype control
1
Antibody Inhibition of G-CSFR in C. perfringens Infection
2
Antibody Labeling with Chelating Agent
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Rat anti-mouse anti-F4/80-A3-1 antibody (AbD Serotec, Kidlington, UK; monoclonal; clone Cl:A3-1) and rat IgG2b isotype control (R&D Systems, Abingdon, UK) were dialysed against 1:1 phosphate-buffered saline (PBS):water to remove sodium azide. Antibodies were conjugated under strict metal-free conditions with isothiocyanatobenzyl-diethylenetriaminepentaacetic acid (ITC-DTPA, Macrocyclics, Dallas, TX, USA) in 0.1 M NaHCO3, pH 9.5, using a 10-fold molar excess of ITC-DTPA for 1 h at room temperature (RT). Unbound ITC-DTPA was removed by dialysis against 0.1 M 2-(N-morpholino)ethanesulfonic acid (MES), pH 5.5.
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