Total cell lysates were separated by 8% or 10% SDS-PAGE and then electroblotted and transferred onto PVDF membranes.31 (link) After incubation with primary antibodies specific for COL1A1 (1:1000; sc-293182, Santa Cruz, TX, USA), CD44 (1:2000; #37259, CST, Boston, USA), E-cadherin (1:2000; #14472, CST, Boston, USA), vimentin (1:2000; #5741, CST, Boston, USA), TGFBI(1:1000; 10188–1-AP, proteintech, IL, USA), MMP9 (1:2000; #15561, CST, Boston, USA) and β-actin (1:4000,sc-81178, Santa Cruz, TX, USA) at 4 °C overnight, the blots were incubated with the corresponding secondary antibodies at room temperature, and the proteins were detected with an ECL kit.
Tgfbi
Manufactured by Proteintech
Sourced in United States
TGFBI is a product offered by Proteintech that is used for research purposes. It is a protein that plays a role in the transforming growth factor-beta (TGF-β) signaling pathway. The core function of TGFBI is to facilitate the study of TGF-β signaling and its associated cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Sc 81178, by Santa Cruz Biotechnology (1 mentions)
Sc 293182, by Santa Cruz Biotechnology (1 mentions)
β actin, by Santa Cruz Biotechnology (1 mentions)
Actin 60008 1 ig, by Proteintech (1 mentions)
Hmgb1 10829 1 ap, by Proteintech (1 mentions)
Enhanced chemiluminescence ecl kit, by Merck Group (1 mentions)
Gapdh 10494 1 ap, by Proteintech (1 mentions)
3 protocols using tgfbi
1
Western Blot Analysis of Extracellular Matrix Proteins
2
Western Blot Analysis of Key Proteins
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The whole cell lysates were harvested and subjected to SDS-PAGE (10% separation gel) and transferred to a polyvinylidene difluoride (PVDF) membrane. Primary antibodies against the following proteins were used: HMGB1 (10829-1-AP; Proteintech), TGFBI (10188-1-AP; Proteintech), GAPDH (10494-1-AP; Proteintech), and actin (60008-1-Ig; Proteintech). Horseradish peroxidase–conjugated secondary antibodies were used (ZSGB-BIO). Signals were detected using an ECL (enhanced chemiluminescence) kit (Millipore).
3
Immunohistochemical Analysis of HGSOC Biomarkers
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Immunohistochemistry (IHC) analysis was conducted on samples collected from 41 HGSOC patients. It was done according to standard protocols using antibodies against IGFBP4 (ProteinTech), TGFBI (ProteinTech), and serpin family A member 10 (SERPINA10) (ProteinTech); and cytoplasmic staining was considered as positive. Staining was assessed by three independent pathologists (WG, XH, and YW). The immunoreactive score (IRS) gave a range of 0–12 as a product of multiplication between positive cell proportion score (0, no positive cells; 1, <10% of positive cells; 2, 10–50% positive cells; 3, 51%–80% positive cells; and 4, >80% positive cells) and staining intensity score (0, no color; 1, mild staining; 2, moderate staining; 3, intense staining) (13 (link)).
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