Bacto soytone

Manufactured by BD

Sourced in United States

Bacto Soytone is a nutrient medium component used in microbiological applications. It is a hydrolyzed soy protein that provides a source of amino acids, vitamins, and other nutrients to support the growth of microorganisms.

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Lab products found in correlation

Oligonucleotides, by Eurogentec (2 mentions) Bacto yeast extract, by BD (1 mentions) Bacto tryptone, by BD (1 mentions) Bacto peptone, by BD (1 mentions) N 3 oxododecanoyl l hsl 3 oxo c12 hsl, by Merck Group (1 mentions)

Close spelling variants of this product

Soytone

10 protocols using bacto soytone

Enhanced Protein Secretion in Streptomyces lividans

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Chemicals (Sigma), Bacto Soytone (DIFCO Laboratories), DNA enzymes (New England Biolabs) and oligonucleotides (Eurogentec) were used.
The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository [60 (link)] with the dataset identifiers PXD040146.
Submission details:

Project Name: Enhanced protein secretion in reduced genome strains of Streptomyces lividans.

Project accession: PXD040146.

Reviewer account details:

Username: reviewer_pxd040146@ebi.ac.uk.

Password: 1uBKUTp6.

Hamed M.B., Busche T., Simoens K., Carpentier S., Kormanec J., Van Mellaert L., Anné J., Kalinowski J., Bernaerts K., Karamanou S, & Economou A. (2024). Enhanced protein secretion in reduced genome strains of Streptomyces lividans. Microbial Cell Factories, 23, 13.

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Quantitative Protein Analysis by Western Blot

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Chemicals were from Sigma. Bacto Soytone from DIFCO Laboratories. DNA enzymes were from New England Biolabs and oligonucleotides from Eurogentec. Images were scanned in an ImageQuant^TM 300 or a LAS4000 system and analyzed using the ImageQuant TL software to compare the intensity of the protein bands to those of the BSA calibration bands or other control proteins. SDS–PAGE and western blotting is described in the Supplemental Materials.

Hamed M.B., Vrancken K., Bilyk B., Koepff J., Novakova R., van Mellaert L., Oldiges M., Luzhetskyy A., Kormanec J., Anné J., Karamanou S, & Economou A. (2018). Monitoring Protein Secretion in Streptomyces Using Fluorescent Proteins. Frontiers in Microbiology, 9, 3019.

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Enzyme-Containing Extract from Bacillus subtilis

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Example 2

Production of Enzyme-Containing Extract from Bacillus subtilis 6A-1

An 8 hour aerobic culture of Bacillus subtilis 6A-1 was prepared using Minimal Bacillus Media (MBM) (composition: Sucrose 10.0 g/L, K₂HPO₄2.5 g/L, KH₂PO₄2.5 g/L, (NH₄) 2HPO₄1.0 g/L, MgSO₄7H₂O, FeSO₄7H₂O 0.01 g/L, MnSO₄7H₂O 0.007 g/L Reference: Bacillus and related endospore-forming bacteria. Bioscience Portal “Biology-Life Science-Edu-Lecture Notes.” Nov. 9, 2013. Table 2. https://bioscienceportal.wordpress. com/2013/11/09/bacillus-and-related-endospore-forming-bacteria/) which was amended by replacing Sucrose with Dextrose (10 g/L), and adding CaCl₂(0.12 g/L), MnCl₂(0.05 g/L), and 1% (w/v) Bacto Soytone (BD 243620). The bacterium was incubated at 30° C. for 8 hours. At the end of the incubation period, the bacterial cells were separated from the broth using centrifugation followed by filtration using a 0.45 micron filter. Cellulolytic activity was assayed using the method detailed below. The liquid extracts were then subjected to tests for enzyme activity. Results are detailed in FIG. 1.

US11008534B2. Bacteria and enzymes produced therefrom and methods of using same (2021-05-18). AGRI-KING, INC. [US]. Inventors: Gbenga Ayangbile [US], Mary Grzemski [US], James F. Tobey, Jr. [US], David Spangler [US], Lucas Krueger [US].

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Comparative Media Preparation for Microbial Growth

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Three different types of media: soy extract only, casein extract only, and TSB, were prepared. Media containing soy extract only consists of peptic digest of soybean meal (Bacto Soytone, BD; 20 g/L of water), dextrose (2.5 g/L), and NaCl (5 g/L). Media containing only casein extract consists of pancreatic digest of Casein (Bacto Tryptone; 20 g/L of water), dextrose (2.5 g/L) and NaCl (5 g/L). TSB contains pancreatic digest of casein (Bacto Tryptone 17 g/L), Bacto Soytone (3 g/L), dextrose (2.5 g/L), and NaCl (5 g/L).

Darby A., Lertpiriyapong K., Sarkar U., Seneviratne U., Park D.S., Gamazon E.R., Batchelder C., Cheung C., Buckley E.M., Taylor N.S., Shen Z., Tannenbaum S.R., Wishnok J.S, & Fox J.G. (2014). Cytotoxic and Pathogenic Properties of Klebsiella oxytoca Isolated from Laboratory Animals. PLoS ONE, 9(7), e100542.

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Bacillus subtilis Enzyme Extract Production

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Example 2

Production of Enzyme-Containing Extract from Bacillus subtilis 6A-1

An 8 hour aerobic culture of Bacillus subtilis 6A-1 was prepared using Minimal Bacillus Media (MBM) (composition: Sucrose 10.0 g/L, K₂HPO₄2.5 g/L, KH₂PO₄2.5 g/L, (NH₄) 2HPO₄1.0 g/L, MgSO₄7H₂O, FeSO₄7H₂O 0.01 g/L, MnSO₄7H₂O 0.007 g/L Reference: Bacillus and related endospore-forming bacteria. Bioscience Portal “Biology-Life Science-Edu-Lecture Notes.” Nov. 9, 2013. Table 2. https://bioscienceportal.wordpress.com/2013/11/09/bacillus-and-related-endospore-forming-bacteria/) which was amended by replacing Sucrose with Dextrose (10 g/L), and adding CaCl₂) (0.12 g/L), MnCl₂(0.05 g/L), and 1% (w/v) Bacto Soytone (BD 243620). The bacterium was incubated at 30° C. for 8 hours. At the end of the incubation period, the bacterial cells were separated from the broth using centrifugation followed by filtration using a 0.45 micron filter. Cellulolytic activity was assayed using the method detailed below. The liquid extracts were then subjected to tests for enzyme activity. Results are detailed in FIG. 1.

US10604727B2. Bacteria and enzymes produced therefrom and methods of using same (2020-03-31). Agri-King, Inc. [US]. Inventors: Gbenga Ayangbile [US], Mary Grzemski [US], James F. Tobey, Jr. [US], David Spangler [US], Lucas Krueger [US].

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Bacillus subtilis 6A-1 Enzyme Extract Production

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Example 2

Production of Enzyme-Containing Extract from Bacillus subtilis 6A-1

An 8-hour aerobic culture of Bacillus subtilis 6A-1 was prepared using Minimal Bacillus Media (MBM) (composition: Sucrose 10.0 g/L, K₂HPO₄2.5 g/L, KH₂PO₄2.5 g/L, (NH₄) 2HPO₄1.0 g/L, MgSO₄7H₂O, FeSO₄7H₂O 0.01 g/L, MnSO₄7H₂O 0.007 g/L Reference: Bacillus and related endospore-forming bacteria. Bioscience Portal “Biology-Life Science-Edu-Lecture Notes.” Nov. 9, 2013. Table 2. https://bioscienceportal.wordpress.com/2013/11/09/bacillus-and-related-endospore-forming-bacteria/) which was amended by replacing Sucrose with Dextrose (10 g/L), and adding CaCl₂(0.12 g/L), MnCl₂(0.05 g/L), and 1% (w/v) Bacto Soytone (BD 243620). The bacterium was incubated at 30° C. for 8 hours. At the end of the incubation period, the bacterial cells were separated from the broth using centrifugation followed by filtration using a 0.45 micron filter. Cellulolytic activity was assayed using the method detailed below. The liquid extracts were then subjected to tests for enzyme activity. Results are detailed in FIG. 1.

US10683473B2. Bacteria and enzymes produced therefrom and methods of using same (2020-06-16). AGRI-KING, Inc. [US]. Inventors: Gbenga Ayangbile [US], Mary Grzemski [US], James F. Tobey, Jr. [US], David Spangler [US], Lucas Krueger [US].

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Bacterial Strain Cultivation for Inositol Production

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Bacterial strains and oligonucleotide primers used in this study are listed in Supplementary Tables 1 and 2, respectively. Bacterial strains were maintained in lysogeny broth (LB) medium³⁸, which was supplemented with antibiotics, including 5 μg mL⁻¹ chloramphenicol, 0.5 μg mL⁻¹ erythromycin, 5 μg mL⁻¹ kanamycin, 4 μg mL⁻¹ phleomycin, and 100 μg mL⁻¹ spectinomycin, as required. For the production of inositol, bacterial strains were grown at 37 °C with shaking at 180 rpm in Soytone medium, containing 4% (w/v) Bacto Soytone (Becton, Dickinson and Co., Franklin Lakes, NJ, USA), 0.5% (w/v) Bacto yeast extract (Becton, Dickinson and Co.), and 20 g L⁻¹ (w/v) glucose.

Michon C., Kang C.M., Karpenko S., Tanaka K., Ishikawa S, & Yoshida K.I. (2020). A bacterial cell factory converting glucose into scyllo-inositol, a therapeutic agent for Alzheimer’s disease. Communications Biology, 3, 93.

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Fermentation of Algae Strain MI503-AF4

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A slant culture of A. wychmicini MI503-AF4 was inoculated into a 500 ml baffled Erlenmeyer flask containing 110 ml of a seed medium consisting of 2% (w/v) galactose, 2% (w/v) dextrin, 1% (w/v) Bacto Soytone (Becton Dickinson, Franklin Lakes, NJ, USA), 0.5% (w/v) corn steep liquor (Kogo Starch, Chiba, Japan), 1% (w/v) glycerol, 1.8% Daigo’s Artificial Seawater SP (Nihon Pharmaceutical, Tokyo, Japan), 0.2% (w/v) (NH₄)₂SO₄, and 0.2% (w/v) CaCO₃ in deionized water (pH 7.4 before sterilization). The seed culture was incubated on a rotary shaker (200 rpm) at 30 °C for 7 days. The seed culture (2.5 mL) of the strain was then transferred into a 500 ml baffled Erlenmeyer flask containing 110 ml of a producing medium consisting of 2.0% glycerin, 2.0% dextrin, 1.0% Bacto Soytone, 0.3% yeast extract, 0.2 % (NH₄)₂SO₄, and 0.2% CaCO₃ in deionized water (pH 7.4 before sterilization). Fermentation was conducted on a rotary shaker (180 rpm) at 27 °C for 7 days.

Kimura T., Umekita M., Hatano M., Hayashi C., Sawa R, & Igarashi M. (2022). Wychimicins, a new class of spirotetronate polyketides from Actinocrispum wychmicini MI503-A4. The Journal of Antibiotics, 75(10), 535-541.

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Quorum Sensing Signaling Molecules Evaluation

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AHL signals were tested at 1 µM concentrations and included N-butanoyl-l-homoserine lactone (C4-HSL); N-3-oxo-hexanoyl-l-HSL (3-oxo-C6-HSL), N-3-oxo-octanoyl-l-HSL (3-oxo-C8-HSL), N-3-hydroxyoctanoyl-l-HSL (3-hydroxy-C8-HSL), N-3-oxododecanoyl-l-HSL (3-oxo-C12-HSL), and N-(p-coumaroyl)-l-HSL (p-coumaroyl-HSL) (purchased from Sigma-Aldrich, St. Louis, MO, or the University of Nottingham, Nottingham, United Kingdom). The β-naphthylamide and p-nitroanilide amino acid substrates were purchased from Sigma-Aldrich. Bacto-peptone, Bacto-soytone, and Bacto-tryptone were purchased from Becton, Dickinson, and Company (Franklin Lakes, NJ). The HS dipeptide was purchased from both AnaSpec (Fremont, CA) and Sigma-Aldrich. The tripeptides HSS, SHS, and SSH were custom synthesized by Peptide 2.0 (Chantilly, VA).

Schaefer A.L., Oda Y., Coutinho B.G., Pelletier D.A., Weiburg J., Venturi V., Greenberg E.P, & Harwood C.S. (2016). A LuxR Homolog in a Cottonwood Tree Endophyte That Activates Gene Expression in Response to a Plant Signal or Specific Peptides. mBio, 7(4), e01101-16.

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Solid-State Fermentation for Metabolite Production

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A slant culture of MM456M-mF7 was inoculated into a 500 ml baffled Erlenmeyer flask containing 110 ml of seed medium consisting of 2% (w v À 1 ) galactose, 2% (w v À 1 ) dextrin, 1% (w v À 1 ) Bacto-soytone (Becton, Dickinson and Company, Franklin Lakes, NJ, USA), 0.5% (w v À 1 ) corn steep liquor (Kogo Starch, Chiba, Japan), 1% (w v À 1 ) glycerol, 0.2% (w v À 1 ) (NH 4 ) 2 SO 4 , 0.2% (w v À 1 ) CaCO 3 and 1.8% (w v À 1 ) artificial seawater (Nihon Pharmaceutical, Tokyo, Japan) in deionized water (pH 7.4 before sterilization). The seed culture was incubated in a rotary shaker (180 r.p.m.) at 27 °C for 2 days. The producing culture was performed by solid-state fermentation. A total of 7 ml of seed culture was transferred into a 500 ml K-1 flask containing 40 g of a producing medium consisting of pressed barley (15 g) and deionized water (25 g). The fermentation was carried out at 30 °C for 14 days in the dark.

Takehana Y., Umekita M., Hatano M., Kato C., Sawa R, & Igarashi M. (2017). Fradiamine A, a new siderophore from the deep-sea actinomycete Streptomyces fradiae MM456M-mF7. The Journal of antibiotics, 70(5).

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