The Hras Fn14 MEF cancer cachexia model was established as previously reported [7 (link)]. Hras MEF (non-cachectic) or Hras Fn14 MEF (cachectic) cells were subcutaneously injected into C57BL/6 mice with 5 million cells per mouse. Mouse body weights were measured daily. Mice bearing Hras Fn14 MEF tumors were treated with 002 mAb at 10 mg/kg by intraperitoneal injection at day 7. Control mice were injected with the same volume of PBS. For the PC3* cancer cachexia model, 5 million PC3 or PC3* cells were subcutaneously injected into NOD scid gamma (NSG) mice. Mice received mAb 002 therapy twice per week via intraperitoneal injections (10 mg/kg) or PBS control from day 21 to the endpoint. At the endpoint, tumors were removed for transcriptomic analyses. All procedures in this study were performed under the institutes’ AEC approval. Plasma samples were collected by cardiac puncture into Microvette CB 300 K2E tubes (Sarstedt) followed by centrifugation at 2000× g for 5 min.
Microvette cb 300 k2e tubes
Manufactured by Sarstedt
Sourced in Germany
The Microvette CB 300 K2E tubes are primary blood collection tubes designed for the collection of capillary blood samples. These tubes contain the anticoagulant K2-EDTA, which prevents the blood from clotting during the collection and transportation process.
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Lab products found in correlation
3 protocols using microvette cb 300 k2e tubes
1
Cancer Cachexia Mouse Models
2
Tail Vein Blood Collection and Cell Counting
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Peripheral blood was collected from the tail vein using Microvette CB 300 K2E tubes (Sarstedt) and counted using a HEMAVET HV950 cell counter (Drew Scientific).
3
Oral Glucose Tolerance Test in Rats
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After an overnight fast (12 hours), rats received an oral load of glucose (2 g/kg BW) via oral gavage. Blood samples were collected from the rats' tail vein before (t = 0 minute) and 15, 30, 60, 120, and 180 minutes after glucose administration using Microvette CB 300 K2E tubes (Sarstedt, Nuernbrecht, Germany) containing dipeptidyl peptidase IV inhibitor (Millipore, MA, USA). Blood glucose levels were determined using an automated blood glucose reader (YSI2700 SELECT Biochemistry Analyzer, Biocompare, San Francisco, CA, USA). The tubes were immediately placed on ice, and within 1 hour, the plasma was prepared by centrifugation (2,795 ×g at 4℃) using an Eppendorf bench top centrifuge and stored at −80℃ until assay. GT was calculated as area under the curve (AUC) with t0 starting and t180 ending points for each experiment. Results are expressed as glucose excursion (GE) curves and delta AUC of OGTT. OGTT was performed preoperatively and repeated at 2, 4, 8, and 16 weeks postoperatively.
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