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Goat anti pecam1

Manufactured by Santa Cruz Biotechnology
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Goat anti-PECAM1 is a specific antibody that binds to the PECAM1 (Platelet and Endothelial Cell Adhesion Molecule 1) protein. PECAM1 is a cell adhesion molecule expressed on the surface of endothelial cells and platelets, and is involved in cell-cell interactions and signal transduction. This antibody can be used for the detection and analysis of PECAM1 in various applications.

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Lab products found in correlation

Bovine serum albumin (bsa), by Merck Group (1 mentions) Alexa fluor 594 phalloidin, by Thermo Fisher Scientific (1 mentions) Rabbit anti flk1, by Santa Cruz Biotechnology (1 mentions) To alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Nucblue live readyprobes reagent, by Thermo Fisher Scientific (1 mentions) Paraformaldehyde (pfa), by Merck Group (1 mentions) Triton x 100, by Merck Group (1 mentions) Rabbit anti nf κb p65, by Santa Cruz Biotechnology (1 mentions) Mouse anti phospho tyrosine 4g10, by Merck Group (1 mentions) Mouse anti pecam 1, by Cell Signaling Technology (1 mentions) Alexa conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions)

2 protocols using goat anti pecam1

1

Endothelial Cell Immunofluorescence Assay

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Samples (n = 3) were fixed with 4% paraformaldehyde (Sigma-Aldrich), permeabilized with 0.3% Triton X-100 (Sigma-Aldrich) in PBS, and blocked with 5% bovine serum albumin (Millipore). Samples were incubated with primary goat anti-PECAM1 (1:100; Santa Cruz Biotechnology) and rabbit anti-Flk1 (1:100; Santa Cruz Biotechnology) antibodies, followed by washing and incubating with secondary anti-goat conjugated to Alexa Fluor 488 (1:200; Invitrogen) and anti-rabbit conjugated to Alexa Fluor 488 (1:200; Invitrogen) antibodies with or without Alexa Fluor 594 Phalloidin (1:500; Invitrogen) for cytoskeleton staining. Nuclei were counterstained with NucBlue Live ReadyProbes Reagent (Invitrogen). Images were obtained using an LSM780 confocal laser scanning microscopy.
Kang M.L., Kim H.S., You J., Choi Y.S., Kwon B.J., Park C.H., Baek W., Kim M.S., Lee Y.J., Im G.I., Yoon J.K., Lee J.B, & Sung H.J. (2020). Hydrogel cross-linking–programmed release of nitric oxide regulates source-dependent angiogenic behaviors of human mesenchymal stem cell. Science Advances, 6(9), eaay5413.
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2

Immunofluorescence Analysis of NF-κB Signaling Pathway

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Cells were fixed in PBS containing 4% formaldehyde, permeabilized in 0.1% Triton X-100, and blocked in 1% BSA containing 10% goat serum. Primary antibodies (incubated overnight) included rabbit anti- NF-κB p65 (Santa Cruz), mouse anti-phospho-tyrosine (4G10, Millipore), rabbit anti-Nck1/2 (Millipore), mouse anti-Nck (Abcam), goat anti-PECAM-1 (Santa Cruz), mouse anti-PECAM-1 (Cell Signaling Technology). Staining was visualized with Alexa-conjugated secondary antibodies (Invitrogen) and viewed on a Nikon Eclipse Ti inverted fluorescent microscope. Images were taken by using the Photometrics Coolsnap120 ES2 camera and analyzed by the NIS Elements BR 3.00, SP5 imaging software. Greater than 100 cells were counted for nuclear NF-κB for each condition of individual experiments. Proximity ligation assays were performed using manufacturer instructions, counterstained with DAPI and phalloidin, and quantified using NIS Elements software.
Chen J., Leskov I.L., Yurdagul A J.r., Thiel B., Kevil C.G., Stokes K.Y, & Orr A.W. (2015). Recruitment of the adaptor protein Nck to PECAM-1 couples oxidant stress to canonical NF-κB signaling and inflammation. Science signaling, 8(365), ra20.
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