Twist

Manufactured by Qiagen

TWIST is a high-throughput sample preparation system designed for nucleic acid extraction and purification. It automates the entire process from sample lysis to final elution, enabling efficient and consistent sample processing.

Automatically generated - may contain errors

Lab products found in correlation

Gapdh, by Qiagen (2 mentions) Bcl 2, by Qiagen (2 mentions) Bcl xl, by Qiagen (2 mentions) Survivin, by Qiagen (2 mentions) Mmp 9, by Qiagen (2 mentions) Quantifast sybr green pcr master mix, by Qiagen (1 mentions) Ccnd1, by Qiagen (1 mentions) Nestin, by Qiagen (1 mentions) Nanog, by Qiagen (1 mentions) Cd133, by Qiagen (1 mentions) Revertra ace qpcr rt kit, by Toyobo (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) Vimentin, by Qiagen (1 mentions) Quantifast sybr green pcr master mix, by Thermo Fisher Scientific (1 mentions) 7300 thermocycler, by Thermo Fisher Scientific (1 mentions)

2 protocols using twist

Quantitative Gene Expression Analysis

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Total RNA was isolated using a TRIzol Reagent (Invitrogen) and cDNA was synthesized using a ReverTra Ace^® qPCR RT Kit (Toyobo, Osaka Japan). Quantitative real-time PCR (qPCR) was performed using a QuantiFast SYBR Green PCR master mix (Qiagen, Valencia, CA, USA) with an Applied Biosystems 7300 (Life Technologies, Carlsbad, CA, USA). The data were analyzed by comparative C_t quantification and the value for each sample was normalized to the value for the housekeeping GAPDH gene. The primer pairs used in this experiment were BCL-2 (QT00025011), BCL-XL (QT00236712), SURVIVIN (QT01679664), MMP-2 (QT00088396), MMP-9 (QT00040040), FN (QT00038024), ITGAV (QT00051891), VIMENTIN (QT00095795), TWIST (QT00011956), CCND1 (QT00495285), MYC1 (QT00035406), NESTIN (QT01015301), SOX2 (QT00237601), NANOG (QT01844808), OCT4 (QT00210840), CD133 (QT00075586), and GAPDH (QT0007924) were obtained from Qiagen.

Kim B.H., Lee H., Park C.G., Jeong A.J., Lee S.H., Noh K.H., Park J.B., Lee C.G., Paek S.H., Kim H, & Ye S.K. (2020). STAT3 Inhibitor ODZ10117 Suppresses Glioblastoma Malignancy and Prolongs Survival in a Glioblastoma Xenograft Model. Cells, 9(3), 722.

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Quantitative Analysis of Cancer Biomarkers

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Total RNA was extracted using TRIzol reagent and cDNA was synthesized using a ReverTra Ace^® qPCR RT Kit. Quantitative real-time PCR (qPCR) was performed using a QuantiFast SYBR Green PCR master mix with an Applied Biosystems 7300 thermocycler. Data were analyzed using comparative Ct quantification and the value for each sample was normalized to the value for the GAPDH gene. The primers used in this experiment were BCL-2 (QT00025011), BCL-XL (QT00236712), MCL-1 (QT00094122), SURVIVIN (QT01679664), MMP-2 (QT00088396), MMP-9 (QT00040040), TWIST (QT00011956), and GAPDH (QT0007924) were all obtained from Qiagen.

Kim B.H., Lee H., Song Y., Park J.S., Gadhe C.G., Choi J., Lee C.G., Pae A.N., Kim S, & Ye S.K. (2019). Development of Oxadiazole-Based ODZ10117 as a Small-Molecule Inhibitor of STAT3 for Targeted Cancer Therapy. Journal of Clinical Medicine, 8(11), 1847.

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