The following buffers and chemicals were used: erythrocyte lysis buffer (Qiagen, Hilden, Germany), 1 × PBS, made from 10 × PBS (Rockland, Gilbertsville, PA, United States; pH 7.2) using Millipore water, 0.1% cell permeabilization buffer, made from 10 × saponin-based permeabilization buffer (eBioscience), 4% formaldehyde solution in PBS made from 16% paraformaldehyde (EMS, Hatfield, PA, United States), PBS supplemented with 0.5% BSA (PAN Biotech, Aidenbach, Germany), and 0.02% sodium azide (Sigma-Aldrich, St. Louis, MO, United States) (PBS/BSA). Buffers were sterile-filtered through 0.22-μm membranes and stored in Stericup disposable bottles (Merck, Darmstadt, Germany). Blood, urine, and cells were processed in 50-ml, 15-ml, and 5-ml round-bottom polystyrene/polypropylene tubes (Corning, Corning, NY, United States and Sarstedt, Nümbrecht, Germany).
MAXPAR antibody labeling kits, EQ Four element calibration beads, washing and tuning solution, and DNA intercalators were purchased from Fluidigm Corporation (South San Francisco, CA, United States). Pre-conjugated and unlabeled antibodies are summarized in theSupplementary Table S1 .
Cis-Platinum (II)-diamine dichloride (cisplatin) was purchased from Enzo Life Sciences GmbH (Lörrach, Germany). A 25 mM stock solution was prepared in DMSO (Sigma-Aldrich) and aliquots were stored at -20°C.
MAXPAR antibody labeling kits, EQ Four element calibration beads, washing and tuning solution, and DNA intercalators were purchased from Fluidigm Corporation (South San Francisco, CA, United States). Pre-conjugated and unlabeled antibodies are summarized in the
Cis-Platinum (II)-diamine dichloride (cisplatin) was purchased from Enzo Life Sciences GmbH (Lörrach, Germany). A 25 mM stock solution was prepared in DMSO (Sigma-Aldrich) and aliquots were stored at -20°C.