All common chemicals, including methylthiazol-2-yl-3 (4,5-methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), acridine orange (AO), propidium iodine (PI), 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA), and JC-1, were purchased from Sigma (München, Germany). Antibodies against caspase 3, poly(ADP-ribose) polymerase (PARP), PDX-1, MST1, catalase, Nrf2, and PGC-1α were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA); β-actin was from Novus Biotechnology (Littleton, CO, USA); RANK was from Cell Signaling Technology (Danvers, MA, USA); pSer536-p65 was from Abcam (Cambridge, MA, USA); and SOD1, SOD2, and Sirt1 antibodies were from GeneTex (Irvine, CA, USA). Denosumab was purchased from GlaxoSmithKline (London, UK). Lentiviruses carrying MST1, RANK, and Sirt1 specific short hairpin (sh)RNAs were purchased from the Taiwan National RNAi Core Facility. All chemicals were dissolved in phosphate-buffered salt (PBS) solution and stored at 20 °C until use in experiments.
Antibodies against caspase 3
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Antibodies against caspase-3 are laboratory reagents used to detect and quantify the presence of the caspase-3 protein. Caspase-3 is a key enzyme involved in the apoptosis (programmed cell death) pathway. These antibodies can be used in various research techniques, such as Western blotting, immunohistochemistry, and flow cytometry, to study the expression and activity of caspase-3 in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Nupage bis tris precast gel, by Thermo Fisher Scientific (2 mentions)
Iblot dry blotting system, by Thermo Fisher Scientific (2 mentions)
N cadherin, by Cell Signaling Technology (2 mentions)
Pgc 1α, by Santa Cruz Biotechnology (1 mentions)
Catalase, by Santa Cruz Biotechnology (1 mentions)
Pdx 1, by Santa Cruz Biotechnology (1 mentions)
Hrp conjugated secondary antibody, by Cytiva (1 mentions)
Ecl prime kit, by Cytiva (1 mentions)
Integrin β1, by Cell Signaling Technology (1 mentions)
Antibody to β actin, by Merck Group (1 mentions)
Mmp 2, by Cell Signaling Technology (1 mentions)
P erk, by Cell Signaling Technology (1 mentions)
Annexin 5 fitc, by BD (1 mentions)
P akt, by Cell Signaling Technology (1 mentions)
Caspase 8, by Cell Signaling Technology (1 mentions)
Antibodies against β actin, by Merck Group (1 mentions)
Mcl 1, by Abcam (1 mentions)
Caspase 9, by Cell Signaling Technology (1 mentions)
E cadherin, by Cell Signaling Technology (1 mentions)
Livin, by Cell Signaling Technology (1 mentions)
6 protocols using antibodies against caspase 3
1
Molecular Mechanisms in Cell Biology
2
Protein Expression Analysis in Cells
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Antibodies against caspase‐3, CHOP, ubiquitinated proteins, and actin were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Antibodies against PERK, VCP, IRE1α, ATF4, ATF6, XBP1, and XBP1s antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Cell lysates were separated on NuPAGE Bis‐Tris precast gels (Invitrogen) and transferred to PVDF membranes using an iBlot Dry Blotting system (Invitrogen). The membranes were blocked with 5% non‐fat dry milk and incubated with the primary antibodies at 4°C overnight. Then the membranes were incubated with a HRP‐conjugated secondary antibody (Amersham Biosciences, Little Chalfont, UK). The antibody‐bound proteins were visualized using an ECL Prime kit (Amersham Biosciences).
3
Western Blot Analysis of Protein Markers
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Western blotting was performed as described previously [25 (link)]. Briefly, for Western blotting, proteins were extracted using RIPA buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1% sodium deoxycholate, 0.1% SDS, 1mM Na2EDTA, 1% Triton X-100, 1 μg ml-1 leupeptin) supplemented with protease inhibitors and phosphatase inhibitors (Biotool). The lysate was centrifuged at 13000 rpm at 4°C for 15 min. The supernatants were collected and protein concentrations were measured using the BCA method. Proteins were then immunoblotted on the PVDF membrane by standard procedures. Antibodies against RIF1 were obtained from Bethyl Laboratories. Antibodies to Bax, Bcl2, N-Cadherin, MMP2, and Integrin-β1 were purchased from Cell Signaling Technology. Antibodies against caspase3 were obtained from Santa Cruz. Antibody to β-actin was purchased from Sigma-Aldrich and used as a loading control.
4
Apoptosis Pathway Profiling Protocol
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Antibodies against caspase-3 (#56053) and compound ABT-737 were purchased from Santa Cruz Biotechnology, Inc.; antibodies against AKT (#4691), p-AKT (#4060), ERK (#4695), p-ERK (#4370), NF-κB (#8242), p-NF-κB (#3033), E-cadherin (#3195), vimentin (#5741), N-cadherin (#4068), caspase-8 (#4790), caspase-9 (#9504) and IAP family proteinsc-IAP1 (#7065), c-IAP2 (#3130), survivin (#2808), XIAP (#2045) and livin (#5471) were obtained from Cell Signaling Technology, Inc. Antibodies against β-actin (#A2228) and NFE2L3(#HPA055889) were obtained from Merck KGaA. Antibodies against Bcl-2 (#32124), Mcl-1 (#32087), Bad (#32245), and Bak (#32371) were purchased from Abcam. All antibodies were diluted at 1:1000. Annexin V-FITC was purchased from BD Biosciences. JC-1 was obtained from ThermoFisher Scientific, Inc. Doc was purchased from Selleck Chemicals. All other reagents were from Sigma-Aldrich; Merck KGaA. All drugs were dissolved in DMSO and stocked at -80°C.
5
Western Blot Analysis of Apoptosis Markers
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Antibodies against caspase-3, HSP70, HSP90, ubiquitinated proteins and actin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies against caspase-8 and RIP-1 were purchased from Cell Signaling Technology (Beverly, MA, USA). Anti-HSP70 (HSP72) antibody was purchased from Enzo Life Sciences. Cell lysates were prepared using the M-PER mammalian protein extraction reagent (Thermo Scientific Inc., Rockford, IL, USA) after addition of Halt EDTA-free phosphatase inhibitor cocktail and Halt protease inhibitor cocktail (both from Thermo Scientific Inc.). The cell lysates were separated in NuPAGE Bis-Tris precast gels (Invitrogen, Carlsbad, CA, USA) and transferred to PVDF membranes using an iBlot Dry Blotting system (Invitrogen). The membranes were blocked with 5% non-fat dry milk dissolved in Tris-buffered saline (TBS) containing 0.5% Tween-20 (TBS-T) for 1 h at room temperature, followed by incubation with the primary antibodies at 4°C overnight. After washing with TBS-T, the membranes were incubated with a horseradish peroxidase-conjugated secondary antibody (Amersham Biosciences, Oxford, UK) diluted in TBS-T for 2 h at room temperature. The antibody-bound proteins were visualized using an ECL plus kit (Amersham Bioscience).
6
Apoptosis Induction Assay Protocol
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Fluorescein isothiocyanate (FITC)-conjugated PAC-1 was purchased from BD Bioscience (CA, USA). The 5,5',6,6'-tetrachloro-1,1',3,3'tetraethylbenzimidazolcarbocyanine iodide (JC-1) and E64 were purchased from Roche (Indianapolis, IN, USA). FITC-conjugated annexin V was purchased from Jiamay Biotech (Beijing, China). FITC-conjugated anti-mouse CD62P antibody and anti-human CD62P antibody were from Biolegend (San Diego, CA, USA). Antibodies against Caspase-3, Bax, Bak, and Bcl-XL were purchased from Santa Cruz (Santa Cruz, CA, USA). Anti-β-actin was purchased from Sigma-Aldrich (LA, USA). The purified TSST-1 protein was purchased from Toxin Technology Inc (Sarasota, USA) with an over 98% purity.
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