Viable cell numbers were counted based the Trypan blue exclusion (Corning Inc., Corning, NY, USA). Cell apoptosis was detected by staining with Annexin V-Fluorescein isothiocyanate (FITC) and 7-AAD (BioLegend). Briefly, 2 × 105 primary mSMG cells were incubated with recombinant murine IL-22 (20 ng/mL) for 3 days. The cells were subsequently stained with Trypan blue solution for viable cell enumeration, or incubated with Annexin V-FITC plus 7-AAD (BioLegend) for apoptosis detection. The cells were then analyzed using a BD FACSAria III cell sorter/flow cytometer and the Flowjo software to determine the percentage of cells stained positive for Annexin V and/or 7-AAD.
Trypan blue exclusion
Manufactured by Corning
Sourced in United States
Trypan blue exclusion is a laboratory technique used to assess cell viability. It is a dye-based method that exploits the ability of viable cells to exclude the dye, while non-viable cells take up the dye and appear blue under a microscope. This simple and cost-effective method provides a quantitative measure of cell health and is commonly used in various cell culture applications.
Automatically generated - may contain errors
2 protocols using trypan blue exclusion
1
Apoptosis Quantification of Primary mSMG Cells
2
Immune Cell Profiling from Tumor Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Spleens, tumor-draining lymph nodes (TDLN), and tumors were harvested, and single-cell suspensions were prepared as previously described [6 (link),40 (link)]. Cell counts and viability were determined via trypan blue exclusion (Corning; Tewksbury, MA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!