Trusight one library preparation kit

Manufactured by Illumina

The TruSight One Library Preparation Kit is a targeted sequencing solution designed for the enrichment and preparation of DNA libraries for sequencing on Illumina platforms. The kit targets a comprehensive set of clinically relevant genes, providing a streamlined workflow for generating high-quality sequencing libraries.

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Lab products found in correlation

Kapa library quantification kit, by Avantor (1 mentions) Tapestation 2200, by Agilent Technologies (1 mentions) Hiseq 4000 sequencing system, by Illumina (1 mentions) 7900ht sequence detection system, by Thermo Fisher Scientific (1 mentions) Ampure xp bead, by Beckman Coulter (1 mentions) Nextseq 500 sequencer, by Illumina (1 mentions)

2 protocols using trusight one library preparation kit

Illumina Sequencing Library Preparation

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Sequencing was carried out at the Cologne centre for genomics. The purified tagmented DNA was amplified via a 12- or 15-cycle PCR programme using reagents from the Illumina TruSight One Library Preparation Kit. This procedure adds index 1 (i7) and index 2 (i5) information required for multiplexing, as well as common adapters (P5 and P7) for cluster generation and sequencing. To remove unwanted products, the final library was purified using Beckman Coulter Ampure XP Beads. After purification and validation (2200 TapeStation; Agilent Technologies), libraries were quantified by using the KAPA Library Quantification kit (Peqlab) and the 7900HT Sequence Detection System (Applied Biosystems) and subsequently sequenced on an Illumina HiSeq4000 sequencing system with a paired-end 2×75-nt sequencing protocol.

Frerichs A., Engelhorn J., Altmüller J., Gutierrez-Marcos J, & Werr W. (2019). Specific chromatin changes mark lateral organ founder cells in the Arabidopsis inflorescence meristem. Journal of Experimental Botany, 70(15), 3867-3879.

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TruSight One Targeted Sequencing Protocol

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The genomic DNA was prepared for targeted sequencing using the TruSight One library preparation kit (Illumina, San Diego, CA) following the manufacturer’s instructions. Following generation of FASTQ files on a NextSeq 500 sequencer (Illumina), the sequence was aligned to the hg19 reference genome using the Burrows-Wheeler Aligner (BWA) version 0.7.7. The Genome Analysis Toolkit (GATK) version 1.6 was used for variant calling, and Omicia Opal 4.8.0 was used for variant annotation.

Ferreira C.R., Devaney J.M., Hofherr S.E., Pollard L.M, & Cusmano-Ozog K. (2016). Hereditary Fructose Intolerance Mimicking a Biochemical Phenotype of Mucolipidosis: A Review of the Literature of Secondary Causes of Lysosomal Enzyme Activity Elevation in Serum. American journal of medical genetics. Part A, 173(2), 501-509.

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