Oligosaccharide halo formation during seed germination was observed by ruthenium red staining of the pectins formed around seeds. After 20 min of imbibition seeds were treated with a ruthenium red solution (0.25 mg/mL) and incubated for 15 min at room temperature [100 (link)]. Seeds were observed under an Eclipse E-800 epifluorescence microscope (Nikon) and photographed with an ORCA ER monochromatic camera (Hammamatsu) or were observed under an Olympus SZ61 stereoscope and photographed with a Canon 750D camera.
Sz61 stereoscope
Manufactured by Canon
The SZ61 stereoscope is a compact and versatile optical instrument designed for a variety of applications. It features binocular observation, providing a three-dimensional view of the subject matter. The stereoscope is equipped with a zoom system, allowing for adjustable magnification levels. The device is suitable for tasks that require detailed examination or analysis of specimens or small objects.
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Lab products found in correlation
2 protocols using sz61 stereoscope
1
Ruthenium Red Staining of Seed Pectins
2
Emasculation and Pollen Pollination
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Flowers were emasculated 48 h before pollination with appropriate pollen. After 4 h, the pistils were removed and fixed in Carnoy solution overnight and stained with Alexander stain according to [101 (link)]. Stigmas were observed under an Olympus SZ61 stereoscope and photographed with a Canon 750D camera.
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