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Regm bulletkit

Manufactured by Cambrex
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Sourced in United States

The REGM™ BulletKit is a laboratory equipment product designed for research and development applications. It serves as a versatile tool for the efficient preparation and manipulation of samples. The core function of the REGM™ BulletKit is to facilitate the handling and processing of materials in a laboratory setting, without making claims about its intended use.

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Lab products found in correlation

Caki 2, by American Type Culture Collection (1 mentions) Atcc crl 1573, by American Type Culture Collection (1 mentions) Pcs 400 030, by American Type Culture Collection (1 mentions)

3 protocols using regm bulletkit

1

Cultured PTECs, HK-2, and MCs from Diabetic and Normal Cells

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PTECs of a type 2 diabetic (T2D) patient and a normal individual (Lonza Walkersville Inc., MD, USA) were cultured in Clonetics™ REGM™ BulletKit™. Human PTECs (ATCC PCS-400-010) were cultured in renal epithelial cell basal medium (ATCC PCS400030TM) plus 0.5% fetal bovine serum (FBS). Human kidney-2 (HK-2) cells (ATCC®CRL-2190) were culture in keratinocyte serum free medium plus 2% FBS. Cells were treated with normal glucose (NG, 5.5 mM) and HG (25 mM) for the indicated times. Mouse MCs (MMCs, ATCC®CRL-1927) and human embryonic kidney (HEK) 293 cells (ATCC® CRL-1573™) were cultured in Dulbecco's Modified Eagle Medium (DMEM) with NG supplemented with 5% and 10% FBS respectively (Additional file 1: Fig. S1A). For the co-culture of HK-2 cells and MMCs, HK-2 cells and MMCs were cultured in upper inserts (pore size: 3 μm) and low 6 well plate respectively for a 48 h culture.
Tsai Y.C., Kuo M.C., Hung W.W., Wu P.H., Chang W.A., Wu L.Y., Lee S.C, & Hsu Y.L. (2023). Proximal tubule-derived exosomes contribute to mesangial cell injury in diabetic nephropathy via miR-92a-1-5p transfer. Cell Communication and Signaling : CCS, 21, 10.
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2

Cell Line Culturing and Authentication

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A498, 786-0, Achn and Caki-2 cells were obtained from ATCC (Manassas, VA). Cells were cultured with medium supplemented with 10% FBS at 37°C with 5% CO2 as previously described (21 (link)). Human normal renal proximal tubule epithelial cells (RPTEC) were purchased from Clonetics and cultured in REGM media (REGM BulletKit, Clonetics). Cell lines were authenticated by the source banks using short tandem repeat (STR) DNA profiling techniques.
Carew J.S., Espitia C.M., Zhao W., Han Y., Visconte V., Phillips J, & Nawrocki S.T. (2016). Disruption of autophagic degradation with ROC-325 antagonizes renal cell carcinoma pathogenesis. Clinical cancer research : an official journal of the American Association for Cancer Research, 23(11), 2869-2879.
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3

Primary Tubular Epithelial Cell Culture

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Primary proximal tubular epithelial cells (PTECs) of a DM patient and a normal individual (Lonza Walkersville Inc., Walkersville, MD, USA) were cultured in Clonetics™ REGM™ BulletKit™ (CC-3190). Human PTECs (ATCC PCS-400-010) were cultured in Renal Epithelial Cell Basal Medium (ATCC PCS400030™) plus 0.5% Fetal Bovine Serum (FBS), according to the manufacturer’s suggestion. Cells were treated with normal glucose (NG, 6.2 mM) and HG (30 mM) for the indicated times. Human embryonic kidney (HEK) 293 cells (ATCC® CRL-1573™) were purchased from the American Type Culture Collection (Manassas, VA, USA) and were cultured in DMEM containing 10% FBS.
Tsai Y.C., Kuo P.L., Kuo M.C., Hung W.W., Wu L.Y., Chang W.A., Wu P.H., Lee S.C., Chen H.C, & Hsu Y.L. (2018). The Interaction of miR-378i-Skp2 Regulates Cell Senescence in Diabetic Nephropathy. Journal of Clinical Medicine, 7(12), 468.
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