Mueller hinton broth (mhb)

All oligonucleotides were obtained from Sangon Biotech (China). Magnesium chloride hexahydrate was purchased from Tian Li (China). Trizma base was obtained from Sigma-Aldrich (USA). Agarose was purchased from Lonza (USA). DNase I was obtained from Solarbio (China). Lipofectamine 2000 (LP2000) was purchased from Invitrogen (USA). FM4-64 was purchased from Molecular Probes (USA). Phosphate-buffered saline (PBS) and Mueller–Hinton broth (MHB) were purchased from Boster (China) and Land Bridge (China), respectively. The PrimeScript RT Reagent Kit with DNA Eraser and Premix Taq RT-PCR System were from Takara Bio Inc (Japan). Thse water used in all experiments was prepared via a Millipore Milli-Q purification system with a resistivity greater than 18 MΩ cm⁻¹.

All the oligonucleotides were obtained from Sangon Biotech (Shanghai, China). The 2000 bp DNA marker was bought from Takara (Tokyo, Japan). Magnesium chloride hexahydrate was purchased from Tian Li (Tianjin, China). Trizma base was obtained from Sigma-Aldrich (St. Louis, MO, USA). Agarose was bought from Lonza (Rockland, ME, USA). PBS, Mueller–Hinton broth (MHB) and DMEM were purchased from Boster (Wuhan, China), Land Bridge (Beijing, China) and Life technologies (Waltham, MA, USA), respectively. The water used in all experiments was prepared via a Millipore Milli-Q purification system with a resistivity higher than 18 MΩ cm-1.