In vitro CpsC/D autophosphorylation and in vivo phosphorylated proteins in crude extract of S. pneumoniae were immunodetected using mouse anti-phosphotyrosine monoclonal antibody PY-20 (Sigma-Aldrich) at 1/2000. Detection of GFP fusions was performed using a rabbit anti-GFP polyclonal antibody (AMS Biotechnology) at 1/10000. Detection of Enolase was performed using a rabbit anti-enolase polyclonal antibody at 1/50000 [32 (link)]. Proteins fused to a 6-histidines tag were detected using a mouse anti-6His monoclonal antibody (Sigma-Aldrich) at 1/1500. Detection of capsular polysaccharides was performed using a rabbit anti-serotype 2 CPS polyclonal antibody (Statens serum Institute) at 1/2000. A goat anti-rabbit or anti-mouse secondary polyclonal antibody horseradish peroxidase (HRP) conjugated (Biorad) was used at 1/5000 to reveal immunoblots.
Rabbit anti gfp polyclonal antibody
Manufactured by AMS Biotechnology
Rabbit anti-GFP polyclonal antibody is a laboratory reagent used to detect and quantify the presence of green fluorescent protein (GFP) in biological samples. This antibody is produced by immunizing rabbits with GFP and purifying the resulting polyclonal antibodies. It can be used in various immunodetection techniques, such as Western blotting, immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA).
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Lab products found in correlation
2 protocols using rabbit anti gfp polyclonal antibody
1
Immunodetection of Phosphoproteins in S. pneumoniae
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In vitro CpsC/D autophosphorylation and in vivo phosphorylated proteins in crude extract of S. pneumoniae were immunodetected using mouse anti-phosphotyrosine monoclonal antibody PY-20 (Sigma-Aldrich) at 1/2000. Detection of GFP fusions was performed using a rabbit anti-GFP polyclonal antibody (AMS Biotechnology) at 1/10000. Detection of Enolase was performed using a rabbit anti-enolase polyclonal antibody at 1/50000 [32 (link)]. Proteins fused to a 6-histidines tag were detected using a mouse anti-6His monoclonal antibody (Sigma-Aldrich) at 1/1500. Detection of capsular polysaccharides was performed using a rabbit anti-serotype 2 CPS polyclonal antibody (Statens serum Institute) at 1/2000. A goat anti-rabbit or anti-mouse secondary polyclonal antibody horseradish peroxidase (HRP) conjugated (Biorad) was used at 1/5000 to reveal immunoblots.
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In vitro CpsC/D autophosphorylation and in vivo phosphorylated proteins in crude extract of S. pneumoniae were immunodetected using mouse anti-phosphotyrosine monoclonal antibody PY-20 (Sigma-Aldrich) at 1/2000. Detection of GFP fusions was performed using a rabbit anti-GFP polyclonal antibody (AMS Biotechnology) at 1/10000. Detection of Enolase was performed using a rabbit anti-enolase polyclonal antibody at 1/50000 [32 (link)]. Proteins fused to a 6-histidines tag were detected using a mouse anti-6His monoclonal antibody (Sigma-Aldrich) at 1/1500. Detection of capsular polysaccharides was performed using a rabbit anti-serotype 2 CPS polyclonal antibody (Statens serum Institute) at 1/2000. A goat anti-rabbit or anti-mouse secondary polyclonal antibody horseradish peroxidase (HRP) conjugated (Biorad) was used at 1/5000 to reveal immunoblots.
2
In vivo UbK Autophosphorylation Detection
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In vivo UbK autophosphorylation of GFP-UbK trapped proteins of S. pneumoniae was immunodetected using a mouse anti-phosphotyrosine monoclonal antibody 4G10 (Sigma-Aldrich) at 1/2000. Detection of GFP fusions was performed using a rabbit anti-GFP polyclonal antibody (AMS Biotechnology) at 1/10000. An anti-mouse secondary polyclonal antibody horseradish peroxidase (HRP) conjugated (Biorad) was used at 1/5000 to reveal immunoblots. Detection of enolase was performed using a rabbit anti-enolase polyclonal antibody at 1/250000.
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