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7500 fast dx instrument

Manufactured by Thermo Fisher Scientific
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The 7500 Fast Dx instrument is a real-time PCR system designed for diagnostic applications. It provides fast and accurate nucleic acid detection and quantification. The instrument features a 96-well block, high-speed thermal cycling, and five-color detection capabilities.

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Lab products found in correlation

7500 sds software, by Thermo Fisher Scientific (1 mentions) Kingfisher flex purification system, by Thermo Fisher Scientific (1 mentions) Nuclisens easymag, by bioMérieux (1 mentions) Amfirivert cdna synthesis platinum master mix, by GenDEPOT (1 mentions) Power sybr green pcr master mix, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

7500 Fast (121 citations) 7500 Fast Dx Real-Time PCR Instrument (61 citations) 7500 instrument (38 citations) 7500 Fast instrument (32 citations) Applied Biosystems™ 7500 Fast Dx Real-Time PCR Instrument (18 citations) 7500 Fast (Dx) (7 citations) 7500 Fast-Dx RT-PCR Instrument (3 citations) ABI 7500 Fast DX instrument (2 citations)

4 protocols using 7500 fast dx instrument

1

COVID-19 Molecular Diagnostic Assay

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Symptomatic patients with a high degree of clinical suspicion for COVID-19 disease were tested in the Molecular Diagnostics Laboratory at Houston Methodist Hospital using a validated assay applied for under Emergency Use Authorization from the US Food and Drug Administration. The assay follows the protocol published by the World Health Organization22 and uses a 7500 Fast Dx instrument (Applied Biosystems, Foster City, CA) and 7500 SDS software version 1.4.1 (Applied Biosystems). Testing was performed on nasopharyngeal or oropharyngeal swabs immersed in universal transport media, bronchoalveolar lavage fluid, or sputum treated with dithiothreitol.
Salazar E., Perez K.K., Ashraf M., Chen J., Castillo B., Christensen P.A., Eubank T., Bernard D.W., Eagar T.N., Long S.W., Subedi S., Olsen R.J., Leveque C., Schwartz M.R., Dey M., Chavez-East C., Rogers J., Shehabeldin A., Joseph D., Williams G., Thomas K., Masud F., Talley C., Dlouhy K.G., Lopez B.V., Hampton C., Lavinder J., Gollihar J.D., Maranhao A.C., Ippolito G.C., Saavedra M.O., Cantu C.C., Yerramilli P., Pruitt L, & Musser J.M. (2020). Treatment of Coronavirus Disease 2019 (COVID-19) Patients with Convalescent Plasma. The American Journal of Pathology, 190(8), 1680-1690.
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2

COVID-19 Detection via WHO-Approved Molecular Assay

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Symptomatic patients with a high degree of suspicion for COVID-19 disease were tested in the Molecular Diagnostics Laboratory at Houston Methodist Hospital using an assay filed for under Emergency Use Authorization (EUA) from the U.S. Food and Drug Administration (27 ). The assay follows the protocol published by the World Health Organization (29 ) and uses a 7500 Fast Dx instrument (Applied Biosystems) and 7500 SDS software (Applied Biosystems). Testing was performed on nasopharyngeal or oropharyngeal swabs immersed in universal transport media (UTM), bronchoalveolar lavage fluid, or sputum treated with dithiothreitol (DTT).
Salazar E., Kuchipudi S.V., Christensen P.A., Eagar T.N., Yi X., Zhao P., Jin Z., Long S.W., Olsen R.J., Chen J., Castillo B., Leveque C., Towers D.M., Lavinder J., Gollihar J.D., Cardona J., Ippolito G.C., Nissly R.H., Bird I.M., Greenawalt D., Rossi R.M., Gontu A., Srinivasan S., Poojary I.B., Cattadori I.M., Hudson P.J., Joselyn N., Prugar L., Huie K., Herbert A., Bernard D.W., Dye J., Kapur V, & Musser J.M. (2020). Relationship between Anti-Spike Protein Antibody Titers and SARS-CoV-2 In Vitro Virus Neutralization in Convalescent Plasma. bioRxiv.
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3

Comparative Evaluation of COVID-19 and Influenza Assays

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All samples were tested using the TaqPath™ COVID-19, FluA, FluB Combo Kit (Thermo Fisher Scientific, Waltham, MA, USA), the Lyra® Influenza A+B Assay (Quidel, San Diego, CA, USA), and the Lyra® SARS-CoV-2 Assay (Quidel, San Diego, CA, USA) according to the manufacturer’s instructions for use. Sample extraction was performed using both the KingFisher™ Flex Purification System (Thermo Fisher Scientific) and the NUCLISENS® EASYMAG® (BioMérieux, Durham, NC, USA). After extraction, all real-time PCR was performed using an Applied Biosystems™ 7500 Fast Dx instrument for both assays. After initial testing, remnant specimens were stored at −80 °C.
Upadhyay P., Reddy J., Proctor T., Sorel O., Veereshlingam H., Gandhi M., Wang X, & Singh V. (2023). Expanded PCR Panel Testing for Identification of Respiratory Pathogens and Coinfections in Influenza-like Illness. Diagnostics, 13(12), 2014.
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4

RNA extraction and qRT-PCR analysis

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The commercial RNA extraction kit (Ambion, Life Technologies, Van Allen Way Carlsbad, CA, USA) was used and cDNA synthesized using the amfiRivert cDNA synthesis platinum master mix (GenDEPOT, Katy, TX, USA, Cat number R5600-200). Real-time PCR (qRT-PCR) was conducted using a 7500 FastDX instrument (Applied Biosystems, MA, USA) and the power SYBR green PCR master mix (Applied Biosystems, Warrington, WA1 4SR, UK, Cat number 4367659). Primer IDs and sequences are shown in Supplementary Table 2.
Chakraborty A., Aziz F., Roh E., Le L.T., Dey R., Zhang T., Rathore M.G., Biswas A.S., Bode A.M, & Dong Z. (2020). Knock-down of the TIM/TIPIN complex promotes apoptosis in melanoma cells. Oncotarget, 11(20), 1846-1861.
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