Whole cell lysates from human G3361 melanoma cells were run in duplicate on SDS polyacrylamide gel and transferred to a polyvinylidene difluoride membrane (GE Healthcare). The membranes were incubated with primary antibodies, i.e. anti-Hexokinase I (clone C35C4), anti-Hexokinase II (clone C64G5), anti-PKM1/2 (clone C103A3), anti-PFKP (clone D4B2), anti-Pyruvate Dehydrogenase (Clone C54G1), anti-GAPDH (clone D16H11), anti-HIF-1α (clone D2U3T), or anti-β-Actin (clone 8H10D10) (Cell Signaling Technology), and subsequently incubated with peroxidase-linked secondary antibody. The reactive bands were detected by using chemiluminescent substrate (Thermo Scientific). Quantitative Western Blot signal intensity analysis was performed using Image StudioTM Lite Software (LI-COR Biosciences).
Anti β actin clone 8h10d10
Manufactured by Cell Signaling Technology
Sourced in United States
Anti-β-Actin (clone 8H10D10) is a monoclonal antibody that specifically recognizes the β-actin protein. It is commonly used as a loading control in Western blotting experiments to normalize protein expression levels.
Automatically generated - may contain errors
Lab products found in correlation
Chemiluminescent substrate, by Thermo Fisher Scientific (1 mentions)
Polyvinylidene difluoride membrane, by GE Healthcare (1 mentions)
Image studio lite software, by LI COR (1 mentions)
Alexa fluor 488 phalloidin, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 488 anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
Hrp conjugated anti mouse igg, by Jackson ImmunoResearch (1 mentions)
Horseradish peroxidase hrp conjugated anti rabbit igg, by Jackson ImmunoResearch (1 mentions)
Anti mouse igg hrp linked secondary antibody, by Cell Signaling Technology (1 mentions)
Alexa fluor 488 donkey anti mouse, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 568 goat anti mouse, by Thermo Fisher Scientific (1 mentions)
Control mouse igg, by Merck Group (1 mentions)
Anti ki67 sc 23900, by Santa Cruz Biotechnology (1 mentions)
3 protocols using anti β actin clone 8h10d10
1
Quantitative Profiling of Glycolytic Enzymes
2
Antibody Reagents for Angiogenesis Analysis
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An anti-chick anosmin-1 rabbit pAb was provided by Dr. Kenneth M. Yamada. The following primary Abs were purchased from commercial companies: an anti-β-actin (clone 8H10D10) mouse mAb, an anti-VEGF Receptor 2 (clone 55B11) rabbit mAb, an anti-phospho-VEGFR2 (Y1175) (clone 19A10) rabbit mAb, an anti-FGFR1 (clone D8E4) rabbit mAb, an anti-phospho-FGF Receptor (Tyr653/654) rabbit pAb, an anti-phospho-PKC substrate rabbit pAb, and an anti-phospho-PLCγ1 (Y783) rabbit pAb from Cell Signaling Technology (Danvers, MA, USA); an anti-PLCγ1 (clone B-4) mouse mAb and an anti-Flk-1 rabbit pAb from Santa Cruz Biotechnology (Dallas, TX, USA); an anti-CD31 (PECAM-1) (clone MEC 13.3) rat mAb from BD Pharmingen (Franklin Lakes, NJ, USA); an anti-phospho-VEGFR2 (Y1214) rabbit pAb from St John’s Laboratory (London, UK); and an anti-GAPDH (clone 3H12) mouse mAb from Medical & Biological Laboratories (Nagoya, Japan).
The following secondary Abs were used: horseradish peroxidase (HRP)-conjugated anti-rabbit IgG and HRP-conjugated anti-mouse IgG Abs from Jackson ImmunoResearch (West Grove, PA, USA). Alexa Fluor 594 anti-rat IgG, Alexa Fluor 488 anti-rabbit IgG, and Alexa Fluor 488 Phalloidin were purchased from Thermo Fisher Scientific.
The following secondary Abs were used: horseradish peroxidase (HRP)-conjugated anti-rabbit IgG and HRP-conjugated anti-mouse IgG Abs from Jackson ImmunoResearch (West Grove, PA, USA). Alexa Fluor 594 anti-rat IgG, Alexa Fluor 488 anti-rabbit IgG, and Alexa Fluor 488 Phalloidin were purchased from Thermo Fisher Scientific.
3
Characterization of CSPG4-expressing Cells
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The mouse anti-CSPG4 monoclonal antibody clone 9.2.27 (#554275) was purchased from BD Biosciences. The donkey anti-mouse Alexa Fluor 488® (#A-21202) and goat anti-mouse Alexa Fluor 568® (#A-11004) secondary IgG antibodies were obtained from Life Technologies Corporation. Anti-CD271 (LNGFR) APC-conjugated human monoclonal antibody (#130-091-884) was purchased from MACS Miltenyi Biotec. The APC mouse IgG1, κ isotype control (FC) antibody (#400122) was obtained from BioLegend, Inc. The mouse monoclonal antibodies anti-Ki67 (sc-23900), as well as mAbs against CSPG4: Anti-NG2 clone G-9 (sc-166251) and anti-NG2 clone LHM 2 (sc-53389), were purchased from Santa Cruz Biotechnology, Inc. The primary anti-human CSPG4 antibody clone 132.38 (#ab50009) was purchased from Abcam. Control mouse IgG (#I8765) was obtained from Sigma-Aldrich (Merck KGaA). The mouse monoclonal antibodies anti-p44/42 MAPK (Erk1/2) clone L34F12 (#4696), anti-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) clone E10 (#9106), anti-Akt (pan) clone 40D4 (#2920), anti-phospho-Akt (Ser473) clone 587F11 (#4051) and anti-β-actin clone 8H10D10 (#3700) were purchased from Cell Signaling Technology, Inc. Anti-mouse IgG, HRP-linked secondary antibody (#7076) was obtained from Cell Signaling Technology, Inc.
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