Ultra 2 directional rna 10 ng

Manufactured by New England Biolabs

The Ultra II Directional RNA (10 ng) is a laboratory product designed for use in RNA sequencing applications. It provides a simple and efficient method for generating directional RNA-seq libraries from low input RNA samples. The product includes all necessary reagents and enzymes for the library preparation process.

Automatically generated - may contain errors

Lab products found in correlation

Tapestation 2200, by Agilent Technologies (3 mentions) Nebnext rrna depletion v2 human mouse rat, by New England Biolabs (3 mentions) Dnase 1, by Zymo Research (3 mentions) Qubit fluorometer, by Thermo Fisher Scientific (3 mentions) Unique dual index primer pairs, by New England Biolabs (2 mentions) Dnase, by New England Biolabs (1 mentions)

Close spelling variants of this product

Ultra II (3 citations)

3 protocols using ultra 2 directional rna 10 ng

RNA Isolation and Directional Library Prep

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

RNA isolation and library preparation is fully described in Butler, et al. (Butler et al., 2021 (link)). Briefly, library preparation on all the nasopharyngeal swab samples’ total nucleic acid (TNA) were treated with DNAse 1 (Zymo Research, Catalog # E1010). Post-DNAse digested samples were then put into the NEBNext rRNA depletion v2 (Human/Mouse/Rat), Ultra II Directional RNA (10 ng), and Unique Dual Index Primer Pairs were used following the vendor protocols from New England Biolabs. Kits were supplied from a single manufacturer lot. Completed libraries were quantified by Qubit or equivalent and run on a Bioanalyzer or equivalent for size determination. Libraries were pooled and sent to the WCM Genomics Core or HudsonAlpha for final quantification by Qubit fluorometer (ThermoFisher Scientific), TapeStation 2,200 (Agilent), and qRT-PCR using the Kapa Biosystems Illumina library quantification kit.

Saravia-Butler A.M., Schisler J.C., Taylor D., Beheshti A., Butler D., Meydan C., Foox J., Hernandez K., Mozsary C., Mason C.E, & Meller R. (2022). Host transcriptional responses in nasal swabs identify potential SARS-CoV-2 infection in PCR negative patients. iScience, 25(11), 105310.

+ Open protocol

+ Expand

RNA Isolation and Library Preparation

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

RNA isolation and library preparation is fully described in Butler et al. (2021) (link). Briefly, library preparation on the all nasopharyngeal swab samples’ total nucleic acid (TNA) were treated with DNase 1 (Zymo Research, Catalog # E1010). Post-DNase digested samples were then put into the NEBNext rRNA depletion v2 (Human/Mouse/Rat), Ultra II Directional RNA (10 ng), and Unique Dual Index Primer Pairs were used following the vendor protocols from New England Biolabs. Kits were supplied from a single manufacturer lot. Completed libraries were quantified by Qubit or equivalent and run on a Bioanalyzer or equivalent for size determination. Libraries were pooled and sent to the WCM Genomics Core or HudsonAlpha for final quantification by Qubit fluorometer (ThermoFisher Scientific), TapeStation 2200 (Agilent), and qRT-PCR using the Kapa Biosystems Illumina library quantification kit.

McDonald J.T., Enguita F.J., Taylor D., Griffin R.J., Priebe W., Emmett M.R., Sajadi M.M., Harris A.D., Clement J., Dybas J.M., Aykin-Burns N., Guarnieri J.W., Singh L.N., Grabham P., Baylin S.B., Yousey A., Pearson A.N., Corry P.M., Saravia-Butler A., Aunins T.R., Sharma S., Nagpal P., Meydan C., Foox J., Mozsary C., Cerqueira B., Zaksas V., Singh U., Wurtele E.S., Costes S.V., Davanzo G.G., Galeano D., Paccanaro A., Meinig S.L., Hagan R.S., Bowman N.M., Wolfgang M.C., Altinok S., Sapoval N., Treangen T.J., Moraes-Vieira P.M., Vanderburg C., Wallace D.C., Schisler J.C., Mason C.E., Chatterjee A., Meller R, & Beheshti A. (2021). Role of miR-2392 in driving SARS-CoV-2 infection. Cell Reports, 37(3), 109839.

+ Open protocol

+ Expand

Efficient RNA-Seq Library Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols

For library preparation, all samples’ total nucleic acid (TNA) were treated with DNAse 1(Zymo Research, Catalog # E1010), which cuts both double-stranded and single-stranded DNA. Post-DNAse digested samples were then put into the NEBNext rRNA depletion v2 (Human/Mouse/Rat), Ultra II Directional RNA (10 ng), and Unique Dual Index Primer Pairs were used following the vendor protocols from New England Biolabs (except for the first flowcell, see Supplementary Figures). Kits were supplied from a single manufacturer lot. Completed libraries were quantified by Qubit or equivalent and run on a Bioanalyzer or equivalent for size determination. Libraries were pooled and sent to the WCM Genomics Core or HudsonAlpha for final quantification by Qubit fluorometer (ThermoFisher Scientific), TapeStation 2200 (Agilent), and qRT-PCR using the Kapa Biosystems Illumina library quantification kit.

Butler D., Mozsary C., Meydan C., Foox J., Rosiene J., Shaiber A., Danko D., Afshinnekoo E., MacKay M., Sedlazeck F.J., Ivanov N.A., Sierra M., Pohle D., Zietz M., Gisladottir U., Ramlall V., Sholle E.T., Schenck E.J., Westover C.D., Hassan C., Ryon K., Young B., Bhattacharya C., Ng D.L., Granados A.C., Santos Y.A., Servellita V., Federman S., Ruggiero P., Fungtammasan A., Chin C.S., Pearson N.M., Langhorst B.W., Tanner N.A., Kim Y., Reeves J.W., Hether T.D., Warren S.E., Bailey M., Gawrys J., Meleshko D., Xu D., Couto-Rodriguez M., Nagy-Szakal D., Barrows J., Wells H., O’Hara N.B., Rosenfeld J.A., Chen Y., Steel P.A., Shemesh A.J., Xiang J., Thierry-Mieg J., Thierry-Mieg D., Iftner A., Bezdan D., Sanchez E., Campion TR J.r., Sipley J., Cong L., Craney A., Velu P., Melnick A.M., Shapira S., Hajirasouliha I., Borczuk A., Iftner T., Salvatore M., Loda M., Westblade L.F., Cushing M., Wu S., Levy S., Chiu C., Schwartz R.E., Tatonetti N., Rennert H., Imielinski M, & Mason C.E. (2021). Shotgun transcriptome, spatial omics, and isothermal profiling of SARS-CoV-2 infection reveals unique host responses, viral diversification, and drug interactions. Nature Communications, 12, 1660.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!