The two strains were cultured on LB medium overnight and inoculated in 35-mm confocal dishes (Solarbio, Beijing, China). The plates were incubated at 37 °C for 24 h and stained with a Filmtracer LIVE/DEAD Biofilm Viability Kit (Invitrogen, Carlsbad, CA, USA) in accordance with the manufacturer’s instructions. Three image stacks were obtained from each sample randomly by CLSM using a Leica TCS SP8 microscope with a Lecia TCS SP8 CSU imaging system. All the experiments were carried out in triplicate.
35 mm confocal dishes
Manufactured by Solarbio
Sourced in China
The 35-mm confocal dishes are specialized cell culture dishes designed for use with confocal microscopy. They feature a thin, optically clear glass bottom that allows for high-quality imaging and observation of cells under a confocal microscope.
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2 protocols using 35 mm confocal dishes
1
Live/Dead Biofilm Viability Assay
2
Biofilm Viability Imaging of Bacterial Strains
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The three strains were cultured on MH agar plates overnight and inoculated in 35‐mm confocal dishes (Solarbio, Beijing, China). The plates were incubated at 37°C for 24 hr and stained with a Filmtracer LIVE/DEAD Biofilm Viability Kit (Invitrogen, Carlsbad, CA, USA) in accordance with the manufacturer's instructions. Three image stacks were obtained from each sample randomly by CLSM using a Leica TCS SP2 microscope with a Lecia TCS SP2/AOBS imaging system.
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