Blood samples were obtained from each mouse after sacrifice. After centrifugation at 4000 rpm with 15 min, the supernatant was obtained as the serum samples. The Cytometric Bead Array (CBA) with a BD CBA Mouse Th1/Th2/Th17 Cytokine Kit (BD Bioscience, Catalog No. 560485) was applied for detection of TNF, IL-2, IFN-γ, IL-6, and IL-17A. Briefly, the same volumes of mixed beads, serum samples, and PE detection antibodies were mixed and incubated with 2 h at 25°C in darkness. Subsequently, the samples were rinsed and then analyzed with a BD LSRF Fortessa flow cytometer with FACP Array Software (BD Biosciences, San Jose, CA, USA).
Facp array software
Manufactured by BD
Sourced in United States
FACP Array software is a tool designed for the management and configuration of fire alarm control panels (FACPs). It provides a centralized interface for monitoring and controlling multiple FACP devices within a system. The software's core function is to enable users to view real-time status updates, configure settings, and generate reports related to the connected FACP equipment.
Automatically generated - may contain errors
Lab products found in correlation
Cba mouse th1 th2 th17 cytokine kit, by BD (1 mentions)
Lsrf fortessa flow cytometer, by BD (1 mentions)
Facsverse flow cytometer, by BD (1 mentions)
Facscanto 2, by BD (1 mentions)
Cell cycle and apoptosis analysis kit, by Beyotime (1 mentions)
Cytometric bead array kit, by BD (1 mentions)
Facscalibur flow cytometer, by BD (1 mentions)
Facsaria 2 flow cytometer, by BD (1 mentions)
4 protocols using facp array software
1
Serum Cytokine Profiling in Mice
2
Cell Cycle Analysis by Flow Cytometry
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The cell cycle assay was performed using the Cell Cycle and Apoptosis Analysis kit (Beyotime Institute of Biotechnology). Cells were seeded into 6-well plates (4×105 cells/well). Following transfection, the cells were collected and washed with PBS. Subsequently, 100 µl RNase A solution was added, then the cells were incubated at 37°C for 30 min. Finally, 400 µl PI was added and incubated at room temperature for 30 min. The DNA content was detected with a FACSVerse™ flow cytometer with FACSCanto II; FACP Array™ software (version 3.0; BD Biosciences).
3
Flow Cytometry Analysis with Cytometric Bead Array
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A BD FACSCalibur flow cytometer and Cytometric Bead Array kits were used according to the manufacturer’s instructions (BD Bioscience, San Jose, CA, USA). The data were collected and calculated using the FACP Array software (BD Bioscience, San Jose, CA, USA).
4
Cytokine Release Profiling of PBMCs
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Approximately 2×105 PBMCs per well were seeded in 100 µl medium in a 96-well microtiter plate and were then treated as shown in Table 1 . The supernatants were collected by centrifugation at 300×g for 5 min. The expression levels of cytokines released in the supernatant were determined using a BD FACSAria II flow cytometer using a BD CBA (Cytometric Bead Array) kit as described in the manufacturer’s instructions (BD Bioscience, San Jose, CA, USA). Data were calculated using the FACP Array software (BD Bioscience, San Jose, CA, USA).
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