Anti β actin antibody 20536 1 ap

Ibandronate sodium (IBAN, s3148), 3BDO and 3-methyladenine (3-MA) were procured from Selleck Chemicals (Shanghai, China). Ang II and phorbol 12-myristate 13-acetate (PMA) was purchased from Sigma-Aldrich (Shanghai, China). Anti-mTOR (cat. no. 2983S), anti-phospho-mTOR (cat. no. 5536S), anti-Rac1-2-3(cat.no. 2465), anti-LC3 (cat. no. 3868S), anti-beclin-1 (cat. no. 3495S), and anti-p62 (cat. no. 8025S) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). The anti-FDPS antibody (ab18987) was procured from Abcam (Cambridge, MA, USA). The anti-β-actin antibody (20536-1-AP) was purchased from Proteintech (Rosemont, MA, USA).

The compounds 1,25-D3, VDR antagonist pyridoxal-5-phosphate (P5P), and 2,3,5-triphenyltetrazolium chloride (TTC) were purchased from Sigma-Aldrich (St. Louis., MO, USA). The primary antibodies anti-VDR antibody (14526-1-AP) and anti-β-actin antibody (20536-1-AP) were purchased from Proteintech (Rosemont, IL, USA). Anti-VEGF antibody (ab1316) and anti-CytC antibody (ab133504) were purchased from Abcam (Cambridge, MA, USA). The primary antibodies anti-COXIV(4850), anti-AMPK (5832), anti-p-AMPK (2535), anti-AKT (4685), anti-p-AKT (4060), anti-GSK-3β (9315), anti-p-GSK-3β (9336), anti-TGF-β (3711S), and anti-P53 (2524) were purchased from Cell Signaling Technology (Danvers, MA, USA).
Secondary antibodies HRP-conjugated AffiniPure Goat Anti-Rabbit (SA00001-2) and HRP-conjugated AffiniPure Goat Anti-Mouse (SA00001-1) were purchased from Proteintech. MCAO surgical nylon monofilaments were 0.38–0.40 mm MSRC40B200PK50 (Shen Zhen RWD Life Science Co., Ltd.).