Mouse monoclonal anti lamp1 h4a3

Rabbit polyclonal DPY30, RBBP5, ASH2L and BIG1 antibodies were from Bethyl Laboratories. Rabbit polyclonal anti-GFP (6556) and mouse monoclonal anti-M6PR (ab2733) were from Abcam. Mouse monoclonal anti-Snx1 was obtained from BD Transduction Laboratories. Mouse monoclonal anti-LC3 was obtained from MBL (clone 4E12, catalogue number M152-3). Rabbit polyclonal anti-spastin 86-340 and CD8 antibodies were produced as previously described (Seaman, 2004 (link); Connell et al., 2009 (link)). Mouse monoclonal anti-LAMP1 (H4A3) was obtained from Santa Cruz Biotechnology. Rabbit polyclonal anti-GAPDH (2118) was from Cell Signalling Technology. Alexa Fluor® 488 and 568 labelled secondary antibodies for immunofluorescence were obtained from Molecular Probes. Peroxidase conjugated secondary antibodies for western blotting were obtained from Sigma.

Rabbit polyclonal anti-spastin (86–340) (raised against a glutathione S-transferase fusion protein that incorporated residues 86–340 of M1-spastin) antibodies were produced as previously described (Connell et al., 2009 (link)). Mouse monoclonal α-tubulin (DM1A) and peroxidase-conjugated secondary antibodies for Western blotting were obtained from Sigma-Aldrich; mouse monoclonal anti-myc (4A6) from EMD Millipore; mouse monoclonal anti-SNX1 (611482) from BD transduction laboratories; rabbit polyclonal anti-GFP (ab6556) and mouse monoclonal anti-M6PR (ab2733) from Abcam; mouse monoclonal anti-LAMP1 (H4A3) from Santa Cruz Biotechnology, Inc.; rabbit polyclonal anti-GAPDH (2118) from Cell Signaling Technology; and Alexa Fluor 488– and 568–labeled secondary antibodies for immunofluorescence from Molecular Probes.

Antibodies used were: rabbit polyclonal anti-TMEM16F (Sigma-Aldrich, HPA038958; IB 1:1000); mouse monoclonal anti-SMS2 [7D10] (Santa Cruz, sc-293384; IB 1:1000); mouse monoclonal anti-nSMase-2 (Santa Cruz, sc-166637; IB: 1:1000); mouse monoclonal anti-LAMP1 [H4A3] (Santa Cruz, sc-20011; IF 1:200); rabbit polyclonal anti-CHMP4B (Proteintech, 13683-1-AP; IF 1:300); mouse monoclonal anti-ALIX [3A9] (Biolegend, 634501, IB 1:1000); mouse monoclonal anti-Actin (Sigma-Aldrich, A1978; IF 1:1200; IB 1:10,000); rabbit monoclonal anti-Na/K-ATPase [EP1845Y] (Abcam, ab-76020; IF 1:600); mouse monoclonal anti-TSG101 [C-2] (Santa Cruz, sc-7964; IB 1:1000); mouse monoclonal anti-V5 [R960-25] (Invitrogen, r96025; IF 1:400; IB 1:1000); rabbit polyclonal anti-GFP (Novus Biologicals, NB600-303; IF 1:250); HRP-conjugated goat anti-mouse IgG (Thermo Fisher Scientific, 31430; IB 1:5000); HRP-conjugated goat anti-rabbit IgG (Thermo Fisher Scientific; 31460; IB 1:5000); Cyanine Cy™2-conjugated donkey anti-mouse IgG (Jackson ImmunoResearch Laboratories, 715-225-150; IF 1:400); Cyanine Cy™2-conjugated donkey anti-rabbit IgG (Jackson ImmunoResearch Laboratories, 711-225-152; IF 1:400); Cyanine Cy™3-conjugated donkey anti-rabbit IgG (Jackson ImmunoResearch Laboratories, 715-165-152; IF 1:400); and Cyanine Cy™3-conjugated donkey anti-mouse IgG (Jackson ImmunoResearch Laboratories, 715-165-150; IF 1:400).