Zinc sulfate

Siraitia grosvenorii seed was bought from Guilin Monk Fruit Corp. Wheat flour was bought from Wudeli Flour Group Co., Ltd. (Xinxiang, China). Yeast was purchased from Angel Yeast Co., Ltd. (Yichang, China). 2, 20-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), rutin, and 1, 1-diphenyl-2-picrylhydrazyl (DPPH) were purchased from Sigma-Aldrich (St. Louis, MO, USA), while potassium sulfate was obtained from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Zinc sulfate, potassium ferrocyanide, absolute ethanol, petroleum ether, hydrochloric acid, sodium dihydrogen phosphate, and disodium hydrogen phosphate were acquired from Tianjin Damao Chemical Reagent Factory (Tianjin, China). All other chemicals were analytical grade.

Oysters were obtained from Guangzhou Huangsha seafood market (Guangzhou, China). Alcalase 2.4L (P4860, ≥2.4 U/g) was obtained from Novozymes Biotechnology Co., Ltd. (Copenhagen, Denmark). Zinc sulfate was purchased from Tianjin Damao Chemical Reagent Co., Ltd. (Tianjin, China). Lead reagent and methylene blue were obtained from Yuanye Biotechnology Co., Ltd. (Shanghai, China). Xylenol orange was purchased from Shanghai Jinsui Biotechnology Co. Ltd. (Shanghai, China). Phosphate-buffered saline (PBS, pH 7.4), penicillin, Dulbecco’s modified Eagle medium (DEME), and streptomycin were purchased from Gibco Life Technologies (Grand Island, NY, USA). Fetal bovine serum (FBS) was purchased from Hangzhou Sijiqing Bioengineering Materials Co. Ltd., (Hangzhou, China). The human hepatocellular carcinoma (HepG₂) cell line was purchased from Southern Medical University. Catalase (CAT), glutathione peroxidase (GSH), superoxide dismutase (SOD), cell cycle, and apoptosis assay kits were purchased from Beyotime Biotechnology Co., Ltd. (Nanjing, China). All reagents used were of an analytical grade.