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Mouse anti-RC2 is a monoclonal antibody that specifically binds to the RC2 antigen. It can be used to detect the presence and distribution of the RC2 antigen in various biological samples.

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3 protocols using mouse anti rc2

1

Immunohistochemistry for Neuronal Markers

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For immunohistochemistry experiments, we followed the protocol published in Garcia-Calero and Scharff (2013 (link)). The primary antibodies used in this study were: rabbit anti-calbindin (1:1000; CB-38, Swant, Bellinzona, Switzerland), rabbit anti-Tbr1 (1:200; sc-48816, Santa Cruz Biotechnology, Inc), mouse anti-RC2 (1:10; Developmental Studies Hybridoma Bank, Iowa City, IA).
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2

Immunostaining of Neural Markers

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For immunostaining we followed protocols published in Ferran et al. (2015 (link)) and Garcia-Calero and Scharff (2013 (link)). The primary antibodies used in this study were: mouse anti-RC2 (1:10; Developmental Studies Hybridoma Bank, Iowa City, IA), mouse anti-TH (1:1000; Novus Biologicals), mouse anti-parvalbumin (1:2000; Sigma-Aldrich), rabbit anti-Calretinin (1:1500, SWANT). Some sections were counterstained with neutral red.
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3

Immunostaining for Transcription Factors

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For immunostaining we followed the protocol published in Garcia-Calero and Scharff (2013 (link)). The primary antibodies used in this study were: rabbit anti-Otp (F. Vaccarino), rabbit anti-Tbr1 (1:200; sc-48816, Santa Cruz Biotechnology, Inc), mouse anti-RC2 (1:10; Developmental Studies Hybridoma Bank, Iowa City, IA, USA).
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