Api id32c identification systems

Forty-seven clinical isolates were collected from various specimens (blood, normally sterile body fluids, deep tissue, genital tract, gastrointestinal tract, respiratory tract) from hospitalized patients in Turin (Italy) during 2019. Yeast isolates were sub-cultured onto CHROMagar Candida (Becton Dickinson, Milan, Italy) to ensure purity. Then, they were identified by the API ID32C identification systems (BioMérieux, Rome, Italy), stored at -80 °C in MicrobanksTM (Pro-Lab Diagnostics, Neston, UK), and sub-cultured at least twice on Sabouraud dextrose agar (SDA, Oxoid, Milan, Italy) at 35 °C for 24 h (Candida and non-Candida spp.). Slowly growing isolates, such as S. cerevisiae and C. neoformans were incubated for 48–72 h.

Seven C. neoformans sensu lato clinical isolates from HIV-infected patients with cryptococcosis, admitted to Amedeo di Savoia Hospital (Turin, Italy) between January 2013 and December 2014, were tested. Yeast isolates were identified by the API ID32C identification systems (BioMérieux, Rome, Italy). Then, they were stored at − 80 °C in Microbanks™ (Pro-Lab Diagnostics, Neston, UK), and sub-cultured at least twice on Sabouraud dextrose agar (SDA, Oxoid, Milan, Italy) at 35 °C for 72 h before testing.