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Lsm 710 meta inverted microscope

Manufactured by Zeiss
Sourced in United States

The LSM 710 META inverted microscope is a high-performance imaging system designed for advanced fluorescence microscopy. It features a flexible and modular design, allowing for customization to meet the specific needs of researchers and scientists. The core function of this microscope is to provide high-resolution, multicolor imaging capabilities, enabling the visualization and analysis of a wide range of biological samples.

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5 protocols using lsm 710 meta inverted microscope

1

Live-cell Imaging of Transfected Proteins

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The cells used for live-cell imaging were plated in 35-mm no, 1.5 glass-bottomed dishes from MatTek prior to transient transfection. Phenol red-free DMEM (Gibco) supplemented with 10% FBS was applied, and imaging was performed using a Zeiss LSM 710 META inverted microscope with a plan apochromat 63×/1.4 oil-immersion objective (Zeiss, White Plains, NY, USA). Expression of full length proteins was confirmed by immunoblotting (Fig. S2).
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2

Stained Cell Imaging with DAPI

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The stained cell suspensions described above were incubated with diaminophenylindole (DAPI) at 1 μg/mL for 10 minutes at room temperature, and placed on glass slides for imaging on an LSM 710 META inverted microscope (Zeiss) at the Vanderbilt Cell Imaging Shared Resource. Data were analyzed using Zen 2011 software.
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3

3D Cardiac Tissue Imaging

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A series of confocal images (z-stack) were acquired sequentially on 100μm cardiac sections. Image stacks were attained for each channel using an LSM 710 META inverted microscope (Zeiss). Images were maximally projected using ZEN or ImageJ software and reconstructed into 3-D Z-series using Imaris (Bitplane) image analysis software.
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4

Live cell imaging in glass dishes

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Cells used for live cell imaging were plated in 35 mm No 1.5 glass bottom dishes from MatTek prior to transient transfection. Phenol red-free DMEM (Gibco) supplemented with 10% FBS was applied, and imaging was performed using a Zeiss LSM 710 META inverted microscope with a planapochromat 63x/1.4 oil-immersion objective (Zeiss, White Plains, NY).
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5

Immunohistochemical Labeling of Calretinin and cFos

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Primary antibodies for 40 μm free-floating sections were mouse anti-calretinin (MAB1568, Millipore, Burlington, MA (38) ) and rabbit anti-cFos (226 003, Synaptic Systems, Goettingen, Germany (39)), both diluted 1:1000. Widefield fluorescent images were acquired with an AF6000 LX fluorescent microscopy system (Leica, Buffalo Grove, IL) equipped with an HCX PL FLUOTAR 10x objective (NA = 0.30).
Confocal images were acquired with an LSM 710 META Inverted microscope (Zeiss, White Plains, NY) equipped with a 20x Plan-Apochromat objective (NA = 0.8). Cell counting was performed blinded from confocal images.
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