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Anti insulin antibody

Manufactured by Novus Biologicals
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Sourced in United States

The Anti-insulin Antibody is a laboratory reagent used for the detection and quantification of insulin in biological samples. It is a highly specific and sensitive tool for researchers studying insulin-related processes and disorders.

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Lab products found in correlation

Streptavidin hrp conjugate, by Thermo Fisher Scientific (1 mentions) Microplate reader, by Molecular Devices (1 mentions) Anti rabbitigg h l alexa fluor 555 conjugate, by Cell Signaling Technology (1 mentions)

2 protocols using anti insulin antibody

1

ELISA for Biotin-Insulin Quantification

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To detect biotin-insulin levels, ELISA plates were coated with 20μg/ml anti-insulin antibody (Clone: 3A6, Novus Biologicals) at 4 C overnight. After blocking in PBS supplemented with 2% fetal bovine serum (FBS), diluted serum samples were added and incubated at room temperature for 2 hours. After washing, streptavidin-HRP conjugates (Thermo Fisher) were added (1:4000) and plates were kept at room temperature for 1 hour. After washing, TMB substrates were added and reactions were stopped by 2M H2SO4. Absorbance at 450nm was read in a microplate reader (Molecular Device).
Cao Y., Kakar P., Hossen M.N., Wu M.X, & Chen X. (2017). Sustained epidermal powder drug delivery via skin microchannels. Journal of controlled release : official journal of the Controlled Release Society, 249, 94-102.
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2

Insulin and C-Peptide Antibody Staining

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Anti-insulin Antibody (1:200) (mouse monoclonal, Novus Biologicals, Littleton, CO, USA), anti-c-peptide antibody (1:100) (rabbit polyclonal; Cell Signaling Technology) were used as the primary antibodies. Anti-mouse immunoglobulin G (IgG; H ‏+ L) Alexa Fluor 488 conjugate (1:200) and anti-rabbit (IgG; H +‏ L) Alexa Fluor 555 conjugate (1:100) (Cell Signaling Technology) were utilized as the secondary antibodies.
El-Halawani S.M., Gabr M.M., El-Far M., Zakaria M.M., Khater S.M., Refaie A.F, & Ghoneim M.A. (2020). Subcutaneous transplantation of bone marrow derived stem cells in macroencapsulation device for treating diabetic rats; clinically transplantable site. Heliyon, 6(5), e03914.
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