All chemicals, such as tris (hydroxymethyl), citric acid, D-glucose, natrium pyruvate, 3-chloro-1,2-propanediol (3-MCPD) as a glycolysis inhibitor, carbonyl cyanide 3-chlorophenylhydrazone (CCCP) as mitochondrial uncoupler were purchased from Sigma-Aldrich (St. Louis, CA, USA). Meanwhile, the ATP bioluminescence assay kit II was purchased from Roche (Mannheim, Germany).
Semen was collected from 3 fertile male Ettawa Crossbred goats using an artificial vagina. Furthermore, the semen from each buck was washed twice by centrifugation at 250 g for 5 min at RT with tris-citrate buffer (pH 7.2) to exclude seminal plasma. All animal work was performed under the approval of the Research Ethics Committee of the Universitas Padjadjaran (approval no. 39/UN6.KEP/EC/2023).
Semen was collected from 3 fertile male Ettawa Crossbred goats using an artificial vagina. Furthermore, the semen from each buck was washed twice by centrifugation at 250 g for 5 min at RT with tris-citrate buffer (pH 7.2) to exclude seminal plasma. All animal work was performed under the approval of the Research Ethics Committee of the Universitas Padjadjaran (approval no. 39/UN6.KEP/EC/2023).