Elx50 microplate strip washer

Manufactured by Agilent Technologies

Sourced in United States

The ELx50™ microplate strip washer from Agilent Technologies is a compact and versatile instrument designed for automated washing of microplates. It is capable of efficiently washing 96-well and 384-well microplates, as well as microplate strips. The ELx50™ utilizes a patented manifold design to ensure consistent and reliable results.

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Lab products found in correlation

Epoch microplate spectrophotometer, by Agilent Technologies (2 mentions) Mouse quantikine elisa kit, by R&D Systems (1 mentions) Gen5 2, by Agilent Technologies (1 mentions) Elispot plate, by Merck Group (1 mentions) Immunocapture 6, by Cellular Technology (1 mentions) Phytohemagglutinin (pha), by Merck Group (1 mentions) Ctl immunospot s6 micro analyzer, by Cellular Technology (1 mentions)

Spelling variants of this product

ELX50 (38 citations) ELx50 Washer (3 citations) Microplate washer (3 citations) Microplate Washer ELx50 (2 citations) ELx50 automatic microplate strip washer (2 citations)

3 protocols using elx50 microplate strip washer

Cytokine Profiling in HTO-Treated Mice

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On the 8th, 9th, 10th, 12th, 14th, and 16th day after the injection of HTO serum samples were prepared from peripheral blood obtained by heart punctures of the anesthetized mice; on each day the blood was collected and pooled from eight mice. The obtained serum samples were frozen at − 70 °C and then, after defrosting, assayed for the levels of:

IL-2, IL-4, IL-6, IL-10, IFN-γ, and TNF-α, using the Mouse Th1/Th2/Th17 CBA Kits (Becton Dickinson, Warsaw, Poland)—with use of flow cytometry;

IL-1β and IL-1Ra, using the respective Quantikine^® ELISA Mouse kits (R&D Systems, Inc., Minneapolis, USA)—with use of ELISA method employing the Epoch™ microplate spectrophotometer (BioTek^® Instruments) and the ELx50™ microplate strip washer (BioTek^® Instruments).

Nowosielska E.M., Cheda A., Zdanowski R., Lewicki S., Scott B.R, & Janiak M.K. (2018). Effect of internal contamination with tritiated water on the neoplastic colonies in the lungs, innate anti-tumour reactions, cytokine profile, and haematopoietic system in radioresistant and radiosensitive mice. Radiation and Environmental Biophysics, 57(3), 251-264.

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Quantifying TGF-β Levels via ELISA

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After defrosting, the obtained serum samples, after defrosting, were assayed for the levels of TGF-β using the Mouse TGF Beta 1 ELISA Kit PicoKineTM (Boster Bio, Pleasanton, CA, USA) with use of ELISA method employing the Epoch™ microplate spectrophotometer (BioTek Instruments, Inc., Winooski, VT, US) and the ELx50™ microplate strip washer (BioTek Instruments). All determinations were performed in triplicates. The obtained data were analyzed with the use of a reader software Gen5 2.0 (BioTek Instruments).

Nowosielska E.M., Cheda A., Pociegiel M., Cheda L., Szymański P, & Wiedlocha A. (2021). Effects of a Unique Combination of the Whole-Body Low Dose Radiotherapy with Inactivation of Two Immune Checkpoints and/or a Heat Shock Protein on the Transplantable Lung Cancer in Mice. International Journal of Molecular Sciences, 22(12), 6309.

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Quantifying Antigen-Specific T Cell Responses

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Patient PBMC were thawed and resuspend at 3×10⁶/ml in AIM-V^® AlbuMAX^® serum-free media supplemented with 1mM L-glutamine. The KTR responders were run in triplicate in media, test wells and Phytohemagglutinin (Sigma-Aldrich, St Louis, MO, USA) as a positive control. The MLR consisted of 3×10⁵ thawed KTR PBMCs plated on an anti IFN-γ antibody (2G1, Thermo-Scientific, Woburn, MA, USA) coated ELISPOT plate (Millipore, Bellerica, MA, USA) co-incubated with 4 or 5 B cells lines at 5×10⁴/per B cell line, 2 B cell lines per well (total of 1×10⁵ B cells/well), or with the 5^th B cell line at 1×10⁵ B cells/well, for 24h. These B cell lines had either 17 or 21 disparate antigens, with the most similar cell lines paired. After which, the plates were handled as per previously published protocol^{23 (link)}, with half the plates washed utilising an ELx50 Microplate strip washer (BioTek, Winooski, VT, USA).
The images captured with Cellular Technology, Ltd (CTL)-ImmunoSpot^® S6 Micro Analyzer (Cellular Technology, Ltd, Shaker Heights, OH, USA) with ImmunoCapture 6.4 software (Cellular Technology, Ltd). The spots counted using CTL ImmunoSpot 5.0 (Cellular Technology, Ltd) and quality controlled by inspecting every well and adjusting sensitivity. The IFN-γ ELISPOT plates were analysed at the Clinical Trials of Organ Transplantation (CTOT) center in Mount Sinai School of Medicine, New York.

Hope C.M., Fuss A., Hanf W., Jesudason S., Coates P.T., Heeger P.S, & Carroll R.P. (2015). Peripheral natural killer cell and allo-stimulated T-cell function in kidney transplant recipients associate with cancer risk and immunosuppression related complications. Kidney international, 88(6), 1374-1382.

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