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Gzmb gb11

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GzmB (GB11) is a recombinant protein produced by BioLegend. It is a key component of the granzyme B enzyme, which is involved in the cytotoxic T cell-mediated apoptosis pathway.

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Lab products found in correlation

Pd 1 eh12.1, by BioLegend (1 mentions) Carboxyfluorescein succinimidyl ester (cfse), by Thermo Fisher Scientific (1 mentions) Hit8a, by BioLegend (1 mentions) Tcr gd fitc b1, by BioLegend (1 mentions) Hit3a, by BioLegend (1 mentions)

2 protocols using gzmb gb11

1

Multiplex Immunostaining Protocol

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GzmB (GB11), PD-1 (EH12.1) and TIM-3(7D3) all Biolegend.
Phetsouphanh C., Phalora P., Hackstein C.P., Thornhill J., Munier C.M., Meyerowitz J., Murray L., VanVuuren C., Goedhals D., Drexhage L., Russell R.A., Sattentau Q.J., Mak J.Y., Fairlie D.P., Fidler S., Kelleher A.D., Frater J, & Klenerman P. (2021). Human MAIT cells respond to and suppress HIV-1. eLife, 10, e50324.
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2

Comprehensive Immunophenotyping of T-cells

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Extracellular staining was performed to evaluate purity, double positive for CD3-phicoeritrin (Hit3a, BioLegend), CD8-FitC (Hit8a, BioLegend), and TCRg/d-FitC (B1, BioLegend). The naive state of the cells was evaluated by low CD45RO (UCHL1, Tonbo) and by high CD45RA (T6D11, Miltenyi Biotec). For cytokine staining, we used IL-2-APC (MQ1-17H12BD, Pharmingen) and Miltenyi Biotec) . To quantify TLR5 (85B152.5, Abcam), extracellular staining was performed. For cytotoxicity markers, GZMB (GB11, BioLegend) and CD107a (H4A3, Miltenyi Biotec) intracellular staining was performed. For assays of cell division, PBMCs were specifically evaluated by double staining with CFSE (21888, Sigma) 50 nM and CD8-Alexa Fluor 405 (MHCD0826, Life Technologies) or only CFSE in purified CD8 + T cells. For assays of oxidative burst, non-fixed CD8 + T cells were stained with 50 nM dihydroethidium (D7008, Sigma) for 20 min at 37 C, then analyzed immediately on the cytometer.
Galindo-Albarrán A.O., López-Portales O.H., Gutiérrez-Reyna D.Y., Rodríguez-Jorge O., Sánchez-Villanueva J.A., Ramírez-Pliego O., Bergon A., Loriod B., Holota H., Imbert J., Hernández-Mendoza A., Ferrier P., Carrillo-de Santa Pau E., Valencia A., Spicuglia S, & Santana M.A. (2016). CD8+ T Cells from Human Neonates Are Biased toward an Innate Immune Response. Cell reports, 17(8).
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