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Alexa fluor 647 goat anti mouse igg antibody

Manufactured by Abcam
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Alexa Fluor 647 goat anti-mouse IgG antibody is a secondary antibody conjugated with the Alexa Fluor 647 fluorescent dye. It is designed to detect and bind to mouse immunoglobulin G (IgG) antibodies in various immunoassays and imaging applications.

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Lab products found in correlation

Hoechst 33342, by Thermo Fisher Scientific (4 mentions) Alexa fluor 488 phalloidin, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) antibody Alexa Fluor 647-conjugated goat anti-mouse IgG (H+L) antibody Alexa Fluor® 647 goat anti-mouse IgG Alexa Fluor 647 goat anti-mouse Alexa Fluor® 647 anti-mouse IgG Anti-mouse Alexa Fluor 647 Alexa Fluor® 647 IgG Alexa Fluor 647 Alexa 647 Anti-IgG mouse

2 protocols using alexa fluor 647 goat anti mouse igg antibody

1

Visualizing Baculovirus Interactions in Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
The cells were seeded in chamber slides and incubated with BV or MNP-BV under designated conditions. After treatment, the cells were fixed in 4% paraformaldehyde for 20 minutes, permeabilized with PBS containing 0.1% Triton for 3 minutes and blocked with 5% BSA for 1 hour at room temperature. BV was detected by incubating the cells with an antibody against vp39 (kindly provided by Prof. Loy Volkman and Dr. Taro Ohkawa) overnight at 4°C followed by an Alexa Fluor 647 goat anti-mouse IgG antibody (Abcam) 48 (link). After that, the cells were stained with Alexa Fluor 488 phalloidin (Thermo Fisher) and Hoechst 33342 (Thermo Fisher). The images were acquired with a confocal microscope (Nikon A1 Confocal Microscope).
Zhu H., Zhang L., Tong S., Lee C., Deshmukh H, & Bao G. (2018). Spatial control of in vivo CRISPR–Cas9 genome editing via nanomagnets. Nature biomedical engineering, 3(2), 126-136.
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2

Visualizing Baculovirus Interactions in Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
The cells were seeded in chamber slides and incubated with BV or MNP-BV under designated conditions. After treatment, the cells were fixed in 4% paraformaldehyde for 20 minutes, permeabilized with PBS containing 0.1% Triton for 3 minutes and blocked with 5% BSA for 1 hour at room temperature. BV was detected by incubating the cells with an antibody against vp39 (kindly provided by Prof. Loy Volkman and Dr. Taro Ohkawa) overnight at 4°C followed by an Alexa Fluor 647 goat anti-mouse IgG antibody (Abcam) 48 (link). After that, the cells were stained with Alexa Fluor 488 phalloidin (Thermo Fisher) and Hoechst 33342 (Thermo Fisher). The images were acquired with a confocal microscope (Nikon A1 Confocal Microscope).
Zhu H., Zhang L., Tong S., Lee C., Deshmukh H, & Bao G. (2018). Spatial control of in vivo CRISPR–Cas9 genome editing via nanomagnets. Nature biomedical engineering, 3(2), 126-136.
+ Open protocol
+ Expand

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